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LUmo Microplate Chemiluminescence Immunoassay( CLIA) System A .. utobio autobio diagnostics an autobio group company | www.autobio-diagnostics.com • 13485 ISO 9001 C E R T I F I E D IVD C LUmo Autobio LUmo is a state of the art microplate luminometer, designed to meet the demanding requirements of today’s microplate based, glow luminescence applications. Product highlights High sensitivity 1 x 10-23 mole HRP … allows detection of lower analyte concentrations, earlier diagnosis of diseases and efficient tracing of treatments. Large dynamic range 0 to 1.6 x 109 RLU … significantly increases assay linearity and enables reliable interpretation of very low and very high analyte concentrations in one test run without additional sample dilution. Very low crosstalk LUmo incorporates a sophisticated, adaptive positioning system, keeping the detector as close as possible above the sample to eliminate instrument related crosstalk and make optimum use of LUmo‘s high sensitivity and dynamic. Speed It takes typically less than 40 seconds to read a 96-well plate at 0.1 sec integration time. With fast Luminol-based reaction systems, high reading speed greatly improves assay precision across a plate. Specifications LUmo Detection system Photomultiplier module Detection mode Glow luminescence Spectral Range 300 - 650nm Dynamic / Indication Range 0 - 1.600.000.000 RLU Measurement unit RLU (Relative Light Units) Sensitivity at 545nm 1 x 10-23 mole HRP Repeatability < 2% Crosstalk < 1 x 10-7 (typical) Measurement time typically <40 seconds at 0.1 sec. integration time for a 96-well plate Integration time 0.1 sec to 10 sec in increments of 0.1sec Plate types 96-well and 48-well (4x12) microplates, max. height 15.2 mm Shaking 3 modes Dimensions 33.5cm x 20.5cm x 47.0 cm (w x h x l) Weight ~13.0kg net Power supply external voltage range 100-240VAC ±10%, 47-63Hz Data connections Serial Interface RS232 (9-pin), USB 2.0 Environmental conditions Operating Temperature: 10°C to 40°C Humidity: 15%-75% RH noncondensing Storage Temperature: -10°C to 50°C Humidity: <95% RH non-condensing Scope of supply Instrument, power supply, power cable, dustcover, serial cable, USB cable, AUTOsoft - Autobio Control and Evaluation Software (Windows®) and user manual on CD AUTOsoft AUTOsoft control and evaluation software Autobio considers software used to control the instruments and perform the required evaluation as integral part of an efficient system, focussed on processing the variety of microplate based applications. AUTOsoft – Autobios powerful Windows® control and evaluation software – is designed to perform the vast majority of routine and scientific applications. Internal quality control function … used to monitor and statistically analyze controls over time and test runs. AUTOsoft features and highlights Plate Layouts … can be defined and saved independent from the test definition using convenient positioning, filling and replicate functions. Quantitative evaluation: Curve fits modes: linear regression, point to point, cubic spline and 4 parameter fit Axis scaling: linear, logarithmic, logit-log axis scaling and extrapolation option Multitest and Kindling test Up to 12 different assays per sample (multitest) or up to 5 subsequently drawn samples of one patient (kindling test) can be processed on one plate. Sample IDs and patient data can either be entered manually, generated automatically, or imported from a file. The corresponding results are displayed together with the sample data or printed in a sample report. Directory …is used to manage commonly used data and simplify routine data entry. Standard curves can be stored and used for subsequent test runs as measured or adjusted by single or dual point calibration. OEM customization and multilingual user interface The user interface of AUTOsoft can easily be customized to match the requirements of OEM customers regarding graphics and language. CLIA Diagnostic System Principle of CLIA Chemiluminescence Immunoassay (CLIA) using Microplate luminometers provides a sensitive, high throughput, and economical way to quantitatively measure antigen in the samples. Luminescence enhancer Application of HRP-luminol system, with the addition of luminescence enhancer, the substrate solution provides a more reliable and sensitive value.analytical procedures whose performance far exceeds that of immunoassays based on the use of radioactive labels. 10000 with enhancer without enhancer 9000 RLU 8000 7000 6000 0 CLIA platform introduction CLIA is the most widely used technical platform Chemiluminescence immunoassay is now established as one of the best alternatives to conventional ELISA for the low concentrations of analytes in complex samples. During the last two decades the technology has evolved into analytical procedures whose performance far exceeds that of immunoassays based on the use of radioactive labels. CLIA ELISA 70s 80s 90s 15 Minute 20 25 2000 Comparison of CLIA and ELISA 2020 Not required long incubation time. No addition of stopping reagent (colorimetric method is required). More economical compared to conventional colorimetric method (Ex: Enzyme-linked immunosorbent assays, ELISA) More sensitive compare to the colorimetric method 30 Luminescence enhancer produces a long-lasting light emission instead of a short peak kinetics The advantage of Autobio CLIA The advantage of CLIA is the increased sensitivity & dynamic range, which allows detection of lower analytes concentrations & earlier diagnosis of disease. The luminol in the tracer reagent will enhance the signal strength a long-lasting glow reaction that is superior to other substrates. This results in an assay up to 1.6x109more sensitive than ELISA. glow flash 65000 50000 RLU 60s 10 80000 RIA 20 th 5 35000 20000 0 5 10 15 Minute 20 25 30 35 High sensitivity and reliability of glow reaction will bring better result in the CLIA test. CLIA Kits Test Menu Items Packaging Cat.no HBsAg 96T CL0310-2 Anti-HBs 96T CL0311-2 HBeAg 96T CL0312-2 Anti-HBe 96T CL0313-2 Anti-HBc 96T CL0314-2 AFP 96T CL0203-2 CEA 96T CL0205-2 PSA 96T CL0206-2 f-PSA 96T CL0208-2 HCG 96T CL0106-2 CA125 96T CL0209-2 CA153 96T CL0210-2 CA199 96T CL0211-2 CA50 96T CL0207-2 SF ( ferritin) 96T CL0212-2 T3 96T CL1001-2 T4 96T CL1002-2 TSH 96T CL1003-2 FT3 96T CL1004-2 FT4 96T CL1005-2 Anti-TG 96T CL1006-2 Anti-TPO 96T CL1007-2 LH (Luteinizing hormone) 96T CL1101-2 FSH( Follicle Stimulating Hormone) 96T CL1102-2 PRL (Prolactin) 96T CL1103-2 P (Progesterone) 96T CL1104-2 T (Testosterone) 96T CL1105-2 96T CL1106-2 Hepatitis Autobio CLIA products Our CLIA kits employ glow-based chemiluminescent reactions. Our series of the kits covers Thyroid function markers, Gonadal hormones, Tumor markers, Diabetes marker, infectious disease and other markers. They can be used to replace ELISA that has been widely used in many research and diagnostic applications. Tumor Markers Thyroid Fertility Hypertension Adrenocorticotropic Hormone (ACTH) 96T CLJ0202 Aldosterone (ALD) 96T CLJ0102 E2 (Estradiol) Cortisol (Cor) 96T CLJ0302 Diabetes Renin 96T CLJ0402 Insulin (INS) 96T CL1201-2 Angiotensin II (A II) 96T CLJ0502 C-peptide (C-P) 96T CL1202-2 Autobio Diagnostics Co., Ltd. offers more than 200 in vitro diagnostic products including CLIA(microplate based CLIA and magnetic particle based CLIA), ELISA, POCT (Point of Care Test), Microbiology . As an ISO 9001, EN ISO13485 and GMP certified manufacturer, Autobio supplies high quality products through its well established sales network and is reknown as a reliable OEM partner. For details please visit www.autobio-diagnostics.com Autobio Diagnostics Co., Ltd. | No. 87 Jingbei Yi Road | National Eco & Tec Development Area | Zhengzhou City | China 450016 T: +86-371-6798-5313 | F: +86-371-6798-5804 | E: [email protected] | W: www.autobio-diagnostics.com flyer Microplate CLIA 20140715e