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Bio202A NU Barral ECG and Peripheral Circulation Before and After Exercise Based on the iWorx Exercise HH-3 Background The goal of this exercise is to measure and compare cardiac and circulatory parameters before and after exercise. Cardiac parameters involve different ECG intervals, as well as the R-wave amplitude (expressing the force of ventricular contraction) and the heart rate. Circulatory parameters include the R-pulse interval (reflecting the speed by which the pulse wave travels) and the pulse wave amplitude. Outline of the exercise Students will record ECG data (via the ECG cable and 3 electrodes) and pulse data (using the pulse pletysmograph) from a healthy volunteer. Detailed instructions as to how to connect to the IWX214 power supply can be found in the iWorx handout. The software used is Labscribe. Below you can see the screenshot corresponding to the “Main window” of the program, where data collection takes place. Data will be collected in 3 channels, from top to bottom: ECG, Pulse, and pulse integral. Three recordings will be made: 1. Before exercise 2. After 3 min “leg exercise” (running etc- it is important to get the heart rate up) 3. After 3 min hand exercise (with a dynamometer). IMPORTANT: After exercise, keep recording until the subject’s HR and breathing return to normal. Make a mark on the recording by hitting Enter every 30 secs. 1 Save your recordings on the desktop with your name/group name. If you are asked to provide a copy of the recording, from “File” use the “Export” option and save your recording as a jpeg file. The subject needs to exercise with the cables/electrodes on so it takes minimal time between exercise and data collection. To start recording, click on the RECORD button. Analysis of data After the recordings have been made and saved, parameters will be calculated. For that, students should click on the “Analysis” icon on the top menu. For a better viewing of the recordings, students can use the Autoscale and Half time/Double time display buttons. In the analysis window, parameters will be calculated placing cursors on designed parts of the recording and reading the corresponding values. For instance, intervals will be usually measured by placing cursors at the beginning and end of the interval (wave), and reading the T2-T1 value corresponding to the appropriate channel. For amplitudes, one cursor is placed on the baseline before the beginning of a wave, and the other at the peak; reading the V2-V1 value. • The R-wave amplitude. To measure the R wave amplitude, place one cursor on the Q wave that precedes the R wave and the second cursor on the peak of the R wave. The value for V2-V1 on the ECG channel is this amplitude. Measure the amplitudes of two additional R waves. • The beat period, which is the time interval between two adjacent R waves (Figure HH3-6 on page HH-3-4). To measure the beat period, place one cursor on the peak of a R wave and the second cursor on the peak of the adjacent R wave. The value for T2-T1 on the ECG channel is the beat period. Measure the beat period for two additional pairs of R waves. • The P-R interval. To measure this time interval, place one cursor at the beginning of the P wave and the second cursor at the beginning of the QRS complex. The value for T2-T1 on the ECG channel is the P-R interval. Measure this time interval for two additional ECG cycles. • The Q-T interval. To measure this time interval, place one cursor at the beginning of the QRS complex and the second cursor at end of the T wave. The value for T2-T1 on the 2 ECG channel is the Q-T interval. Measure this time interval for two additional ECG cycles. • The T-P interval. To measure this time interval, place one cursor at the end of the T wave and the second cursor at the beginning of the P wave. The value for T2-T1 on the ECG channel is the T-P interval. Measure this time interval for two additional ECG cycles. • The R-Pulse interval, which is the time interval between the peak of the R wave and the peak of the pulse wave that follows the R wave (Figure HH-3-6 on page HH-3-4). To measure this interval, place one cursor on the peak of a R wave and the second cursor on the peak of the pulse wave to its right. The value for T2-T1 on any channel is this interval. Measure this interval for two additional ECG/Pulse cycles. • The Pulse wave amplitude. To measure the pulse wave amplitude, place one cursor on the baseline that precedes the pulse wave and the second cursor on the peak of the pulse wave (Figure HH-3-7 on page HH-3-5). The value for V2-V1 on the Pulse channel is this amplitude. Measure this amplitude for two additional pulse waves. Parameters to be calculated for all three recordings: 1. The average R-wave amplitude. 2. The average beat period, in seconds/beat. 3. The heart rate, which is expressed in beats per minute and calculated from the average beat period by using the following equation: Heart Rate (beats/minute) = 60 seconds/minute # seconds/beat 4. The average P-R interval. 5. The average Q-T interval. 6. The average T-P interval. 7. The average R-Pulse interval. 8. The average pulse wave amplitude. You will need all the above data if using this lab for Detailed Lab Report. For the weekly submission, refer to the weekly lab guide. Component (Value to be Measured) How to Measure Value to be Recorded R wave to “lub” interval Place one cursor on the peak of R wave on ECG channel. Place the second cursor on the onset (top of first vertical line) of event marker on Heart Sounds channel. T2 - T1 on either ECG or Heart Sounds channel R wave to “dub” interval Place one cursor on the peak of R wave on ECG channel. Place the second cursor on the offset (top of second vertical line) of event marker on Heart Sounds channel. T2 - T1 on either ECG or Heart Sounds channel R wave Amplitude Place one cursor on the peak of Q wave before R wave and the second cursor on the peak of the R wave on ECG channel. V2 - V1 on ECG channel only P – R Interval Place one cursor at the beginning of P wave and the second T2 - T1 on ECG channel 3 cursor at the beginning of QRS complex on ECG channel. Q - T Interval Place one cursor at the beginning of QRS complex and the second cursor at the end T wave on ECG channel. only T2 - T1 on ECG channel only Place one cursor at the end of the T wave and place the second cursor at the beginning of the following P wave on ECG channel. T2 - T1 on ECG channel only R wave to Pulse Interval Place one cursor on the peak of R wave on ECG channel. Place the second cursor at the peak of the Pulse wave to its right on the Pulse channel. T2 - T1 on either ECG or Pulse channel Pulse wave Amplitude Place one cursor on the baseline before the Pulse wave and the second cursor at the peak of the Pulse wave to its right on the Pulse channel. V2 - V1 on Pulse channel only Place one cursor on the peak of R wave and the second cursor on the peak of adjacent R wave to its right on the EKG channel. T2 - T1 on ECG channel only T - P Interval Beat Period (R – R Interval) At Rest At 0 sec. Measured After ARM Exercise At 60 sec At 120 sec. Mean Heart Rate 4 Measured After LEG Exercise At 0 sec At 60 sec. At 120 sec