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DOMAINS AND KINGDOMS – Chapter 16-17 Archaea, Bacteria, Eukarya – Protista, including Algae, and Fungi Carl Woese based on studies of r-RNA in various groups of living things suggested 3 Domain classification. The arrangement of nucleotides is highly conserved because the mutation rate in r-RNA is very slow. BACTERIA 1 Prokaryotes 2 No Histones 3 RNA polymerases- 3 types 4 introns absent 5 organelles absent ARCHAEA 1 prokaryotes 2 Histones 3 several types 4 sometimes present 5 absent EUKARYA 1 eukaryotes 2 Histones 3 several types 4 present 5 present Viruses, Viroids and Prions: All are acellular infectious agents much smaller than bacteria. Viruses have a genome of RNA or DNA ( single stranded or double stranded) covered by a layer of proteins called Capsid. In animal viruses, the Capsid is further covered by the host membrane studded with viral spikes. This covering is called Envelope. Diseases: Measles, AIDS, Common Cold and Flu etc. Fig 17.6 Viroids lack capsid. They are formed of only RNA. They infect only plant cells. Prions are formed only of proteins. They are rogue misfolded proteins causing normal proteins to change to infectious form. These cause degenerative diseases of brain. Diseases—mad cow disease, Creutzfeld-Jacob disease etc. BACTERIA: Cell Wall: contains Peptidoglycan. It can get stained with crystal violet-Iodine. If the bacteria retain the stain on washing-these are called Gram+. If the stain is washed, the bacteria are stained with Safranin. These are called Gram- bacteria and have a second membrane outside cell wall. Fig 17.3 Forms: 3 main forms exist. 1 Bacillus - rod shaped bacteria 2 Coccus - spherical bacteria 3 Spiral or Curved bacteria Cell Structure: No nucleus. No histones associated with DNA. Ribosomes are smaller than ribosomes of eukaryotes. All membrane bound organelles are absent. Flagella lack 9+2 arrangement. Metabolism: Most bacteria like animals require organic molecules. These are saprobes and release enzymes to absorb food from outside. Besides fungi these are the main decomposers. Cyanobacteria have Chlorophyll a for photosynthesis like algae and plants (oxygenic photosynthesis). Others use hydrogen sulfide instead of water and release sulfur instead of oxygen (anoxygenic photosynthesis). Still others can gain electrons from inorganic chemicals to reduce CO2 (Chemosynthesis). Cyanobacteria have Chlorophyll a and other pigments in 1 thylakoids. Some have only photosynthetic cells like Oscillatoria species and others in addition to photosynthetic cells have Heterocysts are specialized for Nitrogen Fixation, like Nostoc. Reproduction: All reproduce by Binary Fission. DNA is replicated and 2 daughter chromosomes move apart followed by division of cell by plate method. Some bacteria produce Endospores which can tolerate extreme harsh conditions. Examples are Anthrax and Tetanus bacteria. Some bacteria are branched and are best sources of Antibiotic Drugs which kill pathogenic bacteria. Example is Streptomyces bacteria. Antibiotic drugs inhibit protein synthesis in bacteria. ARCHAEA: These prokaryotic organisms of ancient origin live in extreme environment. They share many characteristics, like histones associated with DNA, with eukaryotic organisms. These include extreme Halophiles – these can tolerate very high concentrations of salts; Thermophiles – these can tolerate very high temperatures (100C); Methanogens – these produce methane, the marsh gas as bye-product and live in anaerobic marshes or digestive tracts of animals. Protists Protists were first eukaryotes to evolve. All eukaryotes lacking distinct characters of 3 higher kingdoms are placed in kingdom Protista Most are unicellular. Others are filamentous or colonial. Mitosis, Meiosis and sexual reproduction arose for the first time in this kingdom. All the organelles of plants, fungi and animals arose in this kingdom. The protists also possess many unique organelles not found anywhere else in the 3 domains. Like the 3 higher kingdoms the protists are photosynthetic (like plants) or heterotrophic absorptive (like fungi) or heterotrophic ingestive (like animals). Table 17.2 shows the many kinds of protists and is useful to compare important characters of each group. Algae are now part of kingdom protista. All have Cholorophyl a, and some additional pigments. The chloroplasts in Algae have special organelle, Pyrenoid, to store and metabolize starch. Asexual reproduction can be by flagellated spores called zoospores or spores without flagella. Sexual reproduction is by flagellated gametes. Many algae, like plants, form a non-flagellated egg and a flagellated sperm. This sexual reproduction is called Oogamy. Algae lack multicellular sex organs. Green Algae are most common in fresh water. These have Chlorophyll a and b, store food as starch and have cellulose in cell walls. All characteristics are common with plants. But lack multicellular sex organs covered with sterile jackets and embryos. They have various forms: unicellular-Chlamydomonas; filamentous-Spirogyra; leafy - Ulva or colonial - Volvox with daughter colonies inside. Diatoms are most abundant unicellular forms in oceans. The body is covered with an intricately designed 2-half silica shell. Adults are Diploid and lack cilia or flagella. Main reproduction is by binary fission. Sexual reproduction is by flagellated gametes. The remains of shells produce Diatomaceous earth mined for filtering and abrasive materials. Diatoms are most important producers in biosphere. Dinoflagellates are unicellular covered with cellulose plates. They have 2 flagella placed at right angle to each other. These are important phytoplanktons in oceans. They are also responsible for causing Red Tides, example Gonyaulax. The red tide is caused due to sudden growth due to availability of Nitrogen or Phosphorus brought by rivers. 2 Red Algae are important sources of gelling agents Agar and Carageenan. Porphyra is edible red alga used as a sushi-wrap. These are mainly filamentous or leafy. Besides Chlorophyll a, these have red and blue pigments to give various colors. Red algae are delicate sea weeds. Brown Algae are usually the large sea weeds called Kelps. In addition to Chl a, these have fucoxanthin a brown pigment. Coastal areas have Laminaria and giant marine kelp Macrocystis. Sargasso Sea has floating red algae. Algin a gelatinous material added to ice creams and cream cheese is extracted from brown algae. Euglenoids are unicellular freshwater forms. Many have choloroplasts others lack them. The chloroplasts are surrounded by 3 membranes than the normal 2. The anterior end has vase-like invagination. Out of which emerges a long flagellum, the other is small. Eye-spot is sensitive to detect light. The body is covered by Pellicle formed of protein strips. They can change body shape. A contractile vacuole lies near the anterior end. Reproduction is by longitudinal cell division. Sexual rep is absent. Protozoan Protists These are animal-like-protists. They are motile and heterotrophic ingestive portists. There are 4 main types: Ciliates include protists locomoting with cilia and covered with pellicle. Common example is Paramecium species. It has a Meganucleus and a Micronucleus. These reproduce asexually by transverse cell division. Sexual rep is by Conjugation for which 2 paramecia join temporarily and exchange nuclei and separate. 2 nuclei from different paramecia fuse to result in fertilization. They have 2 contractile vacuoles surrounded by feeding canals. They lack sexual rep. Amoeboids are covered with cell membrane only and locomote by pseudopodia which are formed by flowing cytoplasm. They change, all the time, their body-shape. They feed by phagocytosis. Freshwater forms have contractile vacuoles to expel excess water. Common example is Amoeba proteus. Entamoeba causes dysentery-blood in stools and gingiva-soft bleeding gums. Foraminifera are amoeboids covered with lime-shells called tests with many pores. Radiolarians have internal silica test. The shells of the 2 groups deposit as sedimentary rocks at sea bottom. Zooflagellates locomote with flagella and body is covered by plasma membrane. Most are symbionts and help in digestion of cellulose (stomach of ruminant mammals). Others are parasites and cause diseases. Common example is Trypanosoma which lives in blood plasma of humans and causes sleeping sickness in Africa. It is transmitted by Tse-tse fly. Sporozoans are without any locomotory organelle. At one end of the cell is apical complex. It helps in entry into host. Common example is Plasmodium species which causes Malaria. It is transmitted by females of mosquito, Anopheles species. Malarial parasite completes sexual phase in Anopheles and asexual reproduction in human liver and red blood cells. Molds as Protists: These are fungus-like-protists. They have flagellated cells. 2 types: Water Molds are filamentous and their cell walls are mainly formed from cellulose. Asexual reproduction is by flagellated spores. Sexual rep is by oogamy- an egg and sperm. Common example is Phytophthora which causes potato blight. It caused the Irish famine in 1940. Slime Molds are naked protists which feed by phagotysis. Slime Molds may be multinucleate protoplasmic masses called plasmodium. These are called acellular slime molds. These reproduce 3 asexually by spores formed in walled sporangia in a cluster called fruiting body. Spores develop into amoeboid or flagellated gametes. Life cycle is shown in fig 28.22. Common example is Physarum species. Other slime molds are cellular and live as solitary amoeboid cells. Under unfavorable conditions these can form aggregations called pseudoplasmodium. When favorable conditions return sporangia in a fruiting body produce spores. The spores give rise to single cells to complete the cycle. FUNGI Fungi typically lack flagellated cells. Only exception is Chytrids which have flagellated spores. These are multicellular, heterotrophic-absorptive eukaryotes. The fungus body is called Mycelium, formed of many thread like Hyphae. Zygospore Fungi-Zygomycota are molds with non-septate hyphae. These reproduce asexually by spores. The gametes formed at the tips of special hyphae, fuse to form zygospore, a thick walled zygote. Under favorable conditions zygospore undergoes meiosis and forms spores which produce the mold again. Table 17.3. Common example is Rhizopus the black bread mold. Sac fungi - Ascomycota have septate hyphae. Most of them reproduce asexually by conidia. Conidia are formed in chains by budding. Two hyphae of + and – strains fuse. The resulting hyphae have 2 nuclei in each cell. Fusion takes place in a special structure called Asci which are enclosed in a fruiting body called Ascocarp. Common examples are Morels and Truffles. Club Fungi- Basidiomycota has special bodies called basidia. Fusion of nuclei take place in it and 4 sexual spores are formed externally from each basidium. Spores germinate to form monokaryotic mycelium. The tips of + and – strains of hyphae fuse to form dikaryotic hyphae which form the fruiting body called Ascocarp. Ascocarp bears gills on its under surface. Gills have basidia. Table 17.3 Common examples are Mushrooms, puff-balls. 4