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Photosynthesis Continued (part 3) Quick Re-cap Where does photosynthesis occur? How many stages are included in the process of photosynthesis? What are they and where do they occur? Explain the light-dependent reaction. Chemiosmosis Remember this picture: Chemiosmosis At position B (the electron transport chain), H+ ions are being pulled across the membrane. These ions are unable to escape the lumen except through highly specialized proteins called ATP synthase complexes As H+ ions rush through these complexes, they release energy ATP synthase then uses this energy to convert ADP to ATP Chemiosmosis This process of creating ATP using the energy from an H+ ion gradient is called chemiosmosis Stage 3: The Calvin Cycle The last stage of photosynthesis is carbon fixation This is where high energy organic molecules are formed from CO2 It is referred to the Calvin cycle in honour of Melvin Calvin who discovered this pathway Memorize Me! Stage 3: The Calvin Cycle The Calvin cycle uses ATP molecules and high energy electrons carried by NADPH from the light dependent reactions It uses these molecules to create G3P (a sugar that is used to create glucose) 3 ATPs and 2 NADPHs are consumed for every CO2 that enters the cycle Stage 3: The Calvin Cycle This means that 18 ATP and the electrons and protons carried by 12 NADPH molecules are needed to create one simple sugar molecule like glucose! In order to operate, the Calvin cycle needs CO2 to be readily available in the stroma of the chloroplast https://www.youtube.com/watch?v=0UzMaoaXKaM Summary of Events 1. Light Dependent Reaction - Light strikes photosystem II - Electrons are energized and ready to go - Photolysis occurs replacing electrons that are moving on to stage 2 Summary of Events 2. Electron Transport and ATP Synthesis - Electrons travel down the transport chain losing energy until they reach photosystem I - Electrons are energized again and are used to form NADPH from NADP+ through a redox reaction - Chemiosmosis occurs transporting H+ ions through ATP synthase complexes to create ATP Summary of Events 3. Calvin Cycle - ATP and NADPH are used from previous steps to turn CO2 into glucose and other sugars - C02 is changed into G3P which is used to make glucose https://www.youtube.com/watch?v=sQK3Yr4Sc_k