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Exam Topics for EBIO 1210, Exam 2, by Topic Cellular Energy and Metabolism 1. Be able to define what an organism’s metabolism is 2. Be able to explain the two types of chemical reactions in nature and understand the concept of entropy as it relates to these two reaction types 3. Be able to explain the importance of chemical energy in organisms and ecosystems 4. Know where sugars are used and created in the context of cellular respiration, fermentation, and photosynthesis 5. Be able to link producers and consumers via cycles of energy and carbon flow; know where energy goes (how it is used and how it leaves) as it travels through a food web 6. Be able to relate the structural features of ATP to its function in the 3 major kinds of cellular work 7. Be able to explain how energy is used and transferred in the Na+/K+ pump, as well as in Na+ and glucose co-transport. 8. Be able to place ATP at the intersection between energy-providing and energy-requiring pathways 9. Be able to identify the reason why ATP is not used for longer-term storage of energy Cellular Respiration Glycolysis, citric acid cycle, mitochondrial ATP formation, and fermentation 1. Be able to follow the flow of energy from (i) food molecules to (ii) energy carriers (NADH and FADH2 transferring electrons and H+) and to (iii) the electron transport chain (leading to ATP synthesis) 2. Know the key reactants and final products of each the 3 stages of cellular respiration, as well as where in a cell each process occurs (you do not need to know any enzymes) 3. Be able to identify the terminal electron acceptor of the mitochondrial electron transport chain 4. Be able to apply the model of the hydroelectric dam to ATP formation by ATP synthase 5. Know which classes of macromolecules can be used as fuels in cellular respiration Extracting energy from energy-rich molecules either with or without oxygen 6. Be able to contrast glycolysis plus fermentation vs. glycolysis plus aerobic respiration (citric acid cycle, oxidative phosphorylation) with respect to (i) their locations, (ii) their need (or lack there of) for oxygen, and (iii) whether they yield little or a lot of energy 7. Be able to identify the function of fermentation and to relate the end products of the 2 types of fermentation to their practical applications 8. Be able to relate fast-twitch muscle fibers to glycolysis/fermentation and slow-twitch muscle fibers to glycolysis/aerobic respiration Photosynthesis 1. Be able to contrast the light collecting reactions with the Calvin cycle with respect to (i) their location within the chloroplast, (ii) their principal roles, and (iii) their starting materials and products 2. Know the source of oxygen produced in photosynthesis and understand the reason why water is split 3. Be able to apply the model of the hydroelectric dam to ATP formation by ATP synthase 1 4. Be able to predict how Calvin cycle enzyme levels change in response to growth of plants in sun or shade 5. Be able to relate the differences between C3, C4, and CAM plants to their ecological advantages and disadvantages Extensions of Photosynthesis and Respiration Review: Compare and contrast photosynthesis and respiration 1. Be able to compare and contrast photosynthesis and mitochondrial respiration with respect to (i) the location of electron transport/ATP synthase versus the carbon cycles, (ii) the mechanism of ATP formation, (iii) the starting materials and products, (iv) the roles of oxygen and water, and (v) the principal role of H (electron and H+) carriers Variations of electron transport and respiration 1. Be able to explain why mitochondria can generate heat and how uncouplers further enhance heat generation 2. Be able to explain why carbon monoxide and cyanide are toxic 3. Understand what rigor mortis is and how it relates to (i) normal muscle function, (ii) ATP, and (iii) fermentation. 2