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Agilent 6230 Accurate-Mass TOF LC/MS Exceptional MS performance without compromises Product Note The Agilent 6230 Accurate-Mass Time-ofFlight (TOF) LC/MS system features Agilent Jet Stream Thermal Focusing technology for significantly improved sensitivity, as well as enhanced MassHunter Workstation software for superior data mining and analysis capabilities. These new features, coupled with Agilent’s True High-Definition TOF (True Hi-Def TOF) technology, enable the 6230 Accurate-Mass TOF to deliver exceptional sensitivity, excellent mass accuracy, fast data acquisition, and streamlined qualitative and quantitative analyses to meet your most challenging research needs. Agilent 6230 Accurate-Mass TOF LC/MS integrates Agilent True Hi-Def TOF technology, Agilent Jet Stream Thermal Focusing technology, and MassHunter Workstation software for sensitive, accurate-mass MS analyses. Product Note True Hi-Def TOF Technology for Exceptional Mass Accuracy, Sensitivity and Speed Sensitive, Accurate-Mass MS Analyses The Agilent 6230 Accurate-Mass TOF LC/MS system is designed to provide superior data quality and advanced analytical capabilities for profiling, identifying, characterizing, and quantifying low molecular-weight compounds and biomolecules with confidence. Integrating three core Agilent technical innovations—True HiDef TOF technology, Agilent Jet Stream Thermal Focusing technology and MassHunter Workstation software— the 6230 TOF platform is ideally suited for accurate-mass analyses of the complex samples encountered in today’s most demanding applications. Agilent True Hi-Def TOF technology, a key feature of Agilent’s 6200 Series TOF LC/MS instruments, delivers outstanding TOF performance characteristics without any performance compromises. • Sub 1-ppm mass accuracy improves confidence and reduces false positives • > 20,000 mass resolution distinguishes target analytes from interferences • Up to 5 orders of in-spectrum dynamic range improves detection of trace-level targets in the presence of high abundant compounds LC sample inlet Nebulizing gas Super-heated sheath gas MS inlet Nozzle voltage Heated drying gas Collimated thermal containment zone results in enhanced MS sensitivity Resistive sampling capillary 2 • Low picogram sensitivity enables identification of very low-abundance compounds • Fast data acquisition rates of up to 40 spectra/second ensure maximum compatibility with fast LC and highthroughput methods • Wide mass range to 20,000 m/z Agilent Jet Stream Technology Enhances LC/MS Sensitivity 5-fold Agilent Jet Stream Thermal Focusing technology significantly increases LC/ MS sensitivity by improving the spatial focusing of electrospray droplets. The subsequent enhancements in ion density and desolvation result in higher MS signal intensities and improved signal-to-noise ratios. On average, a 5-fold improvement in MS sensitivity is realized by using Agilent Jet Stream technology at optimal LC flow rates. Easy to use and tune, Agilent Jet Stream technology is available on the 6230 Accurate-Mass TOF LC/MS to provide maximum sensitivity for multiple applications, including the analysis of drug candidates and trace levels of food contaminants, metabolites or biomarkers. Agilent Jet Stream Thermal Focusing technology utilizes super-heated nitrogen sheath gas to confine the electrospray. Ion density and desolvation are improved, resulting in higher MS signal intensities and reduced noise. Agilent 6230 Accurate-Mass TOF LC/MS 2 x10 +ESI TIC Scan Frag=120.0V Pest MX_20 Lettuce_01 ppb in 1_3 water.d 1 1 1 0.9 0.8 MassHunter Workstation Software for Powerful Data Mining and Analysis Capabilities 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 x105 2.2 2.1 2 1.9 1.8 1.7 1.6 1.5 1.4 1.3 1.2 1.1 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 +ESI Scan (2.501-2.660 min, 13 scans) Frag=120.0V Pest MX_20 Lettuce_01ppb in 1_3 water.d Subtract 390.1763 (M+H)+ 202.0431 Resolving power=15,000 Mass Accuracy=1.3 ppm + 201.1091 204.1217 193.0859 300.2019 234.5509 100 150 200 250 722.4532 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 Counts vs. Mass-to-Charge (m/z) High sensitivity screening (< 1 ppb) of pesticides in lettuce. Thiabendazole and its isotopes are detected at a 0.1 ppb concentration level in the above chromatogram and extracted mass spectrum. x10 4 +ESI Scan (11.590-11.755 min, 11 scans) Frag=225.0V CA-2p.d S x10 6 Agilent’s MassHunter Workstation Software facilitates faster and easier processing of information-rich data generated by the 6230 Accurate-Mass TOF LC/MS. Compound-centric data mining and navigation capabilities enable efficient analyses of complex MS data. A sophisticated molecular feature extraction algorithm automatically retrieves all spectral and chromatographic information for each component in a sample mixture, including those in overlapping and co-eluting peaks, thereby saving hours of analysis time. MassHunter’s integrated mass profiling tools streamline differential and statistical analyses between sample sets. The software can be used to perform additional processing steps such as molecular formula generation, database or library search, deconvolution, isotope pattern matching or charge-state determination for confident compound identification. +ESI Scan (11.590-11.755 min, 11 scans) Frag=225.0V CA-2p.d S … 6.5 5 6 5.5 5 A B 4.5 4 4.5 3.5 4 3.5 3 2.5 3 2.5 2 2 1.5 1.5 1 0.5 1 0.5 0 High performance mass accuracy for bovine carbonic anhydrase confirms protein molecular weight and high resolution (data collected in 4 GHz mode) detects protein modifications. Mass spectrum (A) and the maximum entropy deconvolution (B) of bovine carbonic anhydrase. The calculated average mass of carbonic anhydrase is 29024.85 Da; the mass error is 9 ppm. 3 Designed for the Most Demanding Applications Buy online: www.agilent.com/chem/store The Agilent 6230 Accurate-Mass TOF LC/MS system delivers exceptional MS analyses for applications that demand the highest accurate-mass measurements, without compromising sensitivity and speed. Integration of Agilent Jet Stream Thermal Focusing technology ensures the highest level of sensitivity for your most challenging samples. Powerful new data mining tools in MassHunter Workstation software facilitate profiling, characterization, identification and quantification of compounds in complex mixtures. These performance characteristics enable the 6230 TOF system to support demanding applications such as proteomics, metabolomics, impurity testing, product degradation studies, forensics, food safety, and environmental analyses. Find an Agilent customer center in your country: www.agilent.com/chem/contactus U.S. and Canada 1-800-227-9770 [email protected] Asia Pacific [email protected] Europe [email protected] About Agilent Technologies Agilent Technologies is a leading supplier of life science research systems that enable scientists to understand complex biological processes, determine disease mechanisms, and speed drug discovery. Engineered for sensitivity, reproducibility, and workflow productivity, Agilent’s life science solutions include instrumentation, microfluidics, software, microarrays, consumables, and services for genomics, proteomics, and metabolomics applications. This item is intended for Research Use Only. Not for use in diagnostic procedures. Information, descriptions, and specifications in this publication are subject to change without notice. Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material. © Agilent Technologies, Inc. 2008 Printed in the U.S.A. December 17, 2008 5990-3422EN