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Playing with Punnett Squares SYNOPSIS Students practice using Punnett squares while creating a creature with known alleles for various characteristics. OBJECTIVE Students will be able to demonstrate the accurate use of a Punnett square to determine the genotype and phenotype of a character trait that follows simple dominance inheritance. MATERIALS (one / student or group) • blank paper • colored pencils • one set of parental alleles (see attached) • pencil VOCABULARY inheritance: attributes acquired via biological heredity from the parents Punnett square: a chart that shows all the possible gene combinations for a cross between parents with known genes BACKGROUND Depending on grade level and prior knowledge of students, provide information about genes, alleles, recessive, dominant, and inheritance. The characteristics being examined in these fictional creatures are all transmitted through simple dominance inheritance. This means that there are two alleles at one locus. The dominant allele will dominate over the recessive allele that is masked or covered. PROCEDURES 1. Teach/review content and vocabulary as necessary. Teach students how to create and use Punnett squares. 2. Explain to students they will draw a creature based on given characteristics. They will then need to discern at least one possible genotype each for the female and male parent, then create a Punnett square using their data. Students will circle their creatures’ genotype in each of the Punnett squares they create for each characteristic. The creature should be drawn on one side of the blank paper and the Punnett squares on the other. 3. Divide the students into groups of three to four students. 4. Distribute the necessary materials to the students, including the sheet listing the creatures’ genotypes. a. Explain the worksheet: The creature’s genotype is listed on the left. The dominant and recessive characteristics are listed on the right. The students should draw their creature based on its genotype. b. Students should fill in the male and female parent columns with possibilities of the genotypes. They will use this information to create their Punnett squares. 5. As students work in groups, assist them as necessary. Question them about the drawing of their creature to ensure they are showing the creature’s correct phenotype based on its genotype. Check their Punnett squares for accuracy. CHECK FOR UNDERSTANDING - Ask the students how they were able to determine at least one possible genotype for the female and male parents. - Ask the students the options for parent genotypes if an offspring has a recessive trait (either Aa or aa for both of the parents) - Ask the students what the parents’ genotypes are when the offspring are AA, Aa, aA, and aa (both parents are heterozygous: Aa and Aa) - Ask the students what happens when both parents are homozygous dominant (all offspring are AA) EXTENSION - Show the students how to create a Punnett square that includes multiple characteristics (dihybrid crosses). - Create Punnnett squares crossing the given creatures to find out what the offspring will look like. - Students create their own creatures using the dominant and recessive characteristics given, and then trade them with other students who then guess the genotype of the creature based on its phenotype.