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arch LABORATORY SERVICES Surgery Biological products Experimental protocols Blood products Species and characteristics of the models: All species, reformed or old breeders. For custom age and / or weight, please contact us. Description of our service Our method Whole blood consists of a biological fluid, the plasma, which contains all blood cells. These cells or "blood elements" are the red blood cells, the white blood cells and the platelets. Whole blood is not a stable product: it spontaneously coagulates within a few minutes outside of the blood vessels (e.g. in a laboratory tube), generating a semi-solid mass resembling a compact gel called "clot". Whole blood: • Blood sampling with anticoagulant Plasma is a slightly viscous liquid, transparent and yellowish in healthy animals. It is mixed with anticoagulant. The plasma contains clotting proteins unlike serum. Serum: • Blood sampling without any anticoagulant • Latent period for obtaining coagulation • Centrifugation • Supernatant liquid sampling = serum • Freezing Centrifugation allows for separation of the coagulum into a solid and a liquid portion. The remaining liquid, called serum, is close to plasma but with a different composition (no clotting proteins) since it has undergone changes related to coagulation. Plasma: • Blood sampling with anticoagulant • Centrifugation • Supernatant liquid sampling = plasma • Freezing Rat serum for embryo culture is serum taken from outbred rats without CO2 anesthesia, with a specific centrifugation protocol. Possible options www.janvier-labs.com Our additional offer LaboResearch service Research models Transgenic service Labo service Anticoagulants: • EDTA • Heparin (salts), lithium salt, ammonium salt, sodium salt: beware when titrating sodium • Citrate • Glycolytic inhibitors fluoride or iodoacetate. Our added value Traceability of samplings. Daily production of blood products. Adaptation of the protocol to the needs and objectives of the researcher. Ethical review process by our IACUC (MESR No. 078). Any type of packaging. Transgenic service Transgenic services 2013-06-ENG-LS-03