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LABORATORY SERVICES
Surgery
Biological products
Experimental protocols
Blood products
Species and characteristics of the models:
All species, reformed or old breeders.
For custom age and / or weight, please contact us.
Description of our service
Our method
Whole blood consists of a biological fluid, the plasma, which
contains all blood cells. These cells or "blood elements" are the red
blood cells, the white blood cells and the platelets. Whole blood
is not a stable product: it spontaneously coagulates within a few
minutes outside of the blood vessels (e.g. in a laboratory tube),
generating a semi-solid mass resembling a compact gel called
"clot".
Whole blood:
• Blood sampling with anticoagulant
Plasma is a slightly viscous liquid, transparent and yellowish
in healthy animals. It is mixed with anticoagulant. The plasma
contains clotting proteins unlike serum.
Serum:
• Blood sampling without any anticoagulant
• Latent period for obtaining coagulation
• Centrifugation
• Supernatant liquid sampling = serum
• Freezing
Centrifugation allows for separation of the coagulum into a solid
and a liquid portion. The remaining liquid, called serum, is close
to plasma but with a different composition (no clotting proteins)
since it has undergone changes related to coagulation.
Plasma:
• Blood sampling with anticoagulant
• Centrifugation
• Supernatant liquid sampling = plasma
• Freezing
Rat serum for embryo culture is serum taken from outbred rats
without CO2 anesthesia, with a specific centrifugation protocol.
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Anticoagulants:
• EDTA
• Heparin (salts), lithium salt, ammonium salt, sodium salt: beware when titrating sodium
• Citrate
• Glycolytic inhibitors fluoride or iodoacetate.
Our added value
Traceability of samplings.
Daily production of blood products.
Adaptation of the protocol to the needs and
objectives of the researcher.
Ethical review process by our IACUC (MESR No. 078).
Any type of packaging.
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2013-06-ENG-LS-03