Download Cell structure and function

Microscopy; cell structure and
function
Lab 3
Identifying parts of microscope
The compound light microscope
• Two sets of lenses
—the ocular lenses
—the objective lenses
• The light only passes through clear portions, not
opaque portions
• The objects must be small or thinly sliced
sections
• Total magnification = magnification of the ocular
lens × magnification of the objective lens
Field of view
Field of view
Depth of field
Cell theory
• Hooke, Leeuwenhoek, Schleiden, Schwann, Virchow led to
the development of the cell theory.
• All living things or organisms are made of cells and their
products.
• New cells are created by old cells dividing into two.
• Cells are the basic building units of life.
• All cells are enclosed by a semi-permeable membrane—
plasma membrane
Prokaryotic cell
• Bacteria(cyanobacteria) and archaea
• No membrane-bound nucleus and organelles
Eukaryotic cells
• Eukaryotic cells contain membrane-bound nuclei
and other organelles.
• Eukaryotic cells are structurally more complex
than prokaryotic cells.
Cell structure
• Under the regular light microscope
Plasma membrane
Nucleus – synthesis of DNA&RNA
Endoplasmic reticulum (ER)
Golgi Apparatus
Mitochondrion—energy center
Unique structure of animal cells
—centrioles
Chloroplasts
Lab work
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Procedure 3.1
Procedure 3.2
Alternate Procedure 3.3
Procedure 3.4
Procedure 3.5 omit step 6
Procedure 3.6 Demo Observation only
Procedure 4.1
Procedure 4.3, 4.4 step 1
Procedure 4.5 use IKI instead of neutral red