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Cell Structures Cell Theory • • • All organisms are composed of one or more cells. Cells are the smallest living units of all living organisms. Cells arise only by division of a previously existing cell. Generalized Eukaryotic Cell Cell Size • Most cells are relatively small because as size increases, volume increases much more rapidly (this increases diffusion time). Visualizing Cells Prokaryotic Cells Eukaryotic Cells Characterized by compartmentalization by an endomembrane system, and the presence of membrane-bound organelles. • Nucleus Repository for genetic material • Directs activities of the cell • Surface of nucleus bound by two phospholipid bilayer membranes nuclear membrane Nuclear pores Nucleus Chromosomes • DNA of eukaryotes is divided into linear chromosomes. Endomembrane System Endoplasmic reticulum • Largest internal membrane • Composed of Lipid bilayer • Serves as system of channels outward from the nucleus • Functions in storage and secretion • • Rough ER is “rough” because of associated ribosomes (sites of protein synthesis Smooth ER – has no ribosomes – contained embedded enzymes (for synthesis of carbohydrate and lipid molecules) Ribosomes Ribosomes are RNA-protein complexes composed of two subunits that join and attach to messenger RNA. – site of protein synthesis – assembled in nucleoli Golgi Apparatus Collects, packages, and distributes molecules synthesized at one location in the cell and utilized at another location. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. cis face Proteins trans face Transport vesicle Golgi apparatus LYSOSOMES Membrane-bound vesicles containing digestive enzymes (broken off from Golgi Bodies) Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Cytoplasm Endoplasmic reticulum Phagocytosis Food vesicle Golgi apparatus Lysosomes Plasma membrane Extracellular fluid Digestion of phagocytized food particles or cells Transport vesicle Old or damaged organelle Breakdown of old organelle Organelles With DNA • • Mitochondria – bounded by exterior and interior membranes – interior partitioned by cristae Chloroplasts – have enclosed internal compartments of stacked grana, containing thylakoids – found in photosynthetic organisms Mitochondria A. "Powerhouse of the cell" - cellular metabolism B. Structure- outer and inner membranes, cristae C. Have their own DNA Chloroplasts •Chloroplasts are larger and more complex than mitochondria •Grana – closed compartments of stacked membranes •Thylakoids – disc shaped structure – light capturing pigment •Stroma – fluid matrix Endosymbiosis • Endosymbiotic theory suggests engulfed prokaryotes provided hosts with advantages associated with specialized metabolic activities. Theory of Endosymbiosis Evidence for the endosymbiont theory is that mitochondria and chloroplasts: - Are appropriate size to be descendants of eubacteria. - Have inner membranes similar to those on prokaryotic plasma membranes. - Replicate by splitting, as in prokaryotes. - DNA is circular and different from the DNA of the cell's nucleus. - Contain their own components for DNA transcription and translation into proteins . - Have ribosomes similar to prokaryotic ribosomes. - Molecular systematics lend evidence to support this theory. - Many extant organisms are involved in endosymbiotic relationships. Cytoskeleton • Network of protein fibers supporting cell shape and anchoring organelles – Actin filaments Microtubules cell movement – Microtubules Intermediate filaments Hollow tubes Facilitate cell movement Centrioles – barrel shaped organelles occur in pairs – help assemble animal cell’s microtubules Actin – Intermediate filaments Stable - don’t break down Cytoskeleton Plant Cells • • Central vacuole – often found in the center of a plant, and serves as a storage facility for water and other materials Cell wall – primary walls – laid down while cell is growing – middle lamella – glues cells together – secondary walls – inside the primary cell walls after growth Plant Cell Animal Cells • Animal cells lack cell walls. – form extracellular matrix provides support, strength, and resilience