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Version 05/2011 ACTH (Adrenocorticotropic hormone) antibody Product type Catalog Description Immunogen Reacts with Primary antibodies P-Ab0001 Mouse monoclonal [2F6H11] to ACTH ACTH synthetic peptide (residues1-24) conjugated KLH Adrenocorticotropic hormone (ACTH) in tissues from human, dogs, rat, mouse and pig. Tested applications ELISA, IHC, IHC-Fr, IHC-P(It localizes ACTH in immunohistochemical techniques on formalin-fixed, paraffin-embedded or frozen sections). Relevance Background Antigen localization U-ProteinExpress BV Billing Address Hazenakker 18 3994 EJ Houten The Netherlands tel.: +31(0)6-22466793 email: [email protected] Optimal dilutions should be determined by end user Cushing's disease and Addison’s disease High levels of ACTH may occur in the pituitary adenomas, medullary thyroid carcinomas; bronchial carcinoids, pancreatic islet cell tumours and gastric carcinoids. Adrenocorticotropic hormone is a splice variant of the proopiomelanocortin preproprotein. The pro-opiomelanocortin preproprotein gene (alternative names ACTH, CLIP, LPH, MSH, NPP and POC) encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. cytoplasm KvK/Chamber of Commerce: Utrecht 30230407 Bank account: Rabobank Houten 1185.02.271 BTW/VAT: NL8184.94.232.B01 IBAN: NL09RABO0118502271 BIC: RABONL2U Terms of Payment: 30 days after receipt of invoice Page 1 Version 05/2011 Mouse Monoclonal 2F6H11 Protein G purified IgG1 kappa PBS Liquid 0,5 mg/ml 100 g Short term storage at 4° C (1-2 weeks) Long term storage at -20° C (appropriate aliquots) Raised in Clonality Clone number Purity Isotype Light chain type Storage buffer Form Concentration Amount Storage Disease Cushing's disease (pituitary tumor making ACTH) Adrenal tumor "Ectopic" ACTH (ACTH made by a tumor outside the pituitary, usually in the lung) Addison's disease (adrenal damage) Hypopituitarism Cortisol High High High ACTH High Low High Low Low High Low Figure 1: ACTH staining. Note cytoplasmic localization U-ProteinExpress BV Billing Address Hazenakker 18 3994 EJ Houten The Netherlands tel.: +31(0)6-22466793 email: [email protected] KvK/Chamber of Commerce: Utrecht 30230407 Bank account: Rabobank Houten 1185.02.271 BTW/VAT: NL8184.94.232.B01 IBAN: NL09RABO0118502271 BIC: RABONL2U Terms of Payment: 30 days after receipt of invoice Page 2