Download ACTH (Adrenocorticotropic hormone)antibody - U

Version 05/2011
ACTH (Adrenocorticotropic hormone) antibody
Product type
Catalog
Description
Immunogen
Reacts with
Primary antibodies
P-Ab0001
Mouse monoclonal [2F6H11] to ACTH
ACTH synthetic peptide (residues1-24) conjugated KLH
Adrenocorticotropic hormone (ACTH) in tissues from human, dogs, rat,
mouse and pig.
Tested applications
ELISA, IHC, IHC-Fr, IHC-P(It localizes ACTH in immunohistochemical
techniques on formalin-fixed, paraffin-embedded or frozen sections).
Relevance
Background
Antigen localization
U-ProteinExpress BV
Billing Address
Hazenakker 18
3994 EJ Houten
The Netherlands
tel.: +31(0)6-22466793
email: [email protected]
Optimal dilutions should be determined by end user
Cushing's disease and Addison’s disease
High levels of ACTH may occur in the pituitary adenomas, medullary
thyroid carcinomas; bronchial carcinoids, pancreatic islet cell tumours and
gastric carcinoids.
Adrenocorticotropic hormone is a splice variant of the proopiomelanocortin preproprotein. The pro-opiomelanocortin preproprotein
gene (alternative names ACTH, CLIP, LPH, MSH, NPP and POC) encodes
a polypeptide hormone precursor that undergoes extensive, tissue-specific,
post-translational processing via cleavage by subtilisin-like enzymes
known as prohormone convertases. There are eight potential cleavage sites
within the polypeptide precursor and, depending on tissue type and the
available convertases, processing may yield as many as ten biologically
active peptides involved in diverse cellular functions. The encoded protein
is synthesized mainly in corticotroph cells of the anterior pituitary where
four cleavage sites are used; adrenocorticotrophin, essential for normal
steroidogenesis and the maintenance of normal adrenal weight, and
lipotropin beta are the major end products. In other tissues, including the
hypothalamus, placenta, and epithelium, all cleavage sites may be used,
giving rise to peptides with roles in pain and energy homeostasis,
melanocyte stimulation, and immune modulation. These include several
distinct melanotropins, lipotropins, and endorphins that are contained
within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in
this gene have been associated with early onset obesity, adrenal
insufficiency, and red hair pigmentation. Alternatively spliced transcript
variants encoding the same protein have been described.
cytoplasm
KvK/Chamber of Commerce: Utrecht 30230407
Bank account: Rabobank Houten 1185.02.271
BTW/VAT: NL8184.94.232.B01
IBAN: NL09RABO0118502271
BIC: RABONL2U
Terms of Payment: 30 days after receipt of invoice
Page 1
Version 05/2011
Mouse
Monoclonal
2F6H11
Protein G purified
IgG1
kappa
PBS
Liquid
0,5 mg/ml
100 g
Short term storage at 4° C (1-2 weeks)
Long term storage at -20° C (appropriate aliquots)
Raised in
Clonality
Clone number
Purity
Isotype
Light chain type
Storage buffer
Form
Concentration
Amount
Storage
Disease
Cushing's disease (pituitary tumor making ACTH)
Adrenal tumor
"Ectopic" ACTH (ACTH made by a tumor outside the pituitary, usually in
the lung)
Addison's disease (adrenal damage)
Hypopituitarism
Cortisol
High
High
High
ACTH
High
Low
High
Low
Low
High
Low
Figure 1:
ACTH staining. Note cytoplasmic localization
U-ProteinExpress BV
Billing Address
Hazenakker 18
3994 EJ Houten
The Netherlands
tel.: +31(0)6-22466793
email: [email protected]
KvK/Chamber of Commerce: Utrecht 30230407
Bank account: Rabobank Houten 1185.02.271
BTW/VAT: NL8184.94.232.B01
IBAN: NL09RABO0118502271
BIC: RABONL2U
Terms of Payment: 30 days after receipt of invoice
Page 2