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Renal Pathology Review Medical Diseases of the Kidney Karlene Hewan-Lowe, MD Brody School of Medicine at East Carolina University Renal Pathology The Osler Institute Review Course Normal Histology Renal Pathology The Osler Institute Review Course Histology Normal Glomerulus • Anastamosing capillary network Architecture is defined with H and E, PAS and Methenamine silver stains • Glomerular capillary wall Fenestrated endothelial cell Glomerular Basement membrane 260 – 340 nm Visceral epithelial cell (Podocyte) • Mesangium Mesangial cells Mesangial matrix Renal Pathology The Osler Institute Review Course Histology TubuloInterstitium and Arteries • Proximal convoluted tubules • Distal convoluted Tubules • Interstitium Architecture defined by Masson’s trichrome stain • Interlobular arteries • Arterioles Renal Pathology The Osler Institute Review Course Histologic Alterations in Glomerular Diseases • The kidney has a limited reaction to a myriad of injurious agents • Glomerular hypercellularity Proliferative glomerulonephritides • Thick glomerular basement membranes • Hyalinization Renal Pathology The Osler Institute Review Course Terms Used in the Evaluation of Glomerular Diseases • Diffuse glomerular changes > 50% • Focal glomerular changes < 50% • Global involvement – single glomerulus • Segmental involvement – single glomerulus Renal Pathology The Osler Institute Review Course Immunofluorescence Microscopy Linear capillary wall staining Anti-GBM disease MIDD Diabetic nephropathy DDD IgG, C3 kappa light chains IgG, albumin C3 Granular mesangial staining IgA Nephropathy ISN/RPS Lupus Class I, II C1q nephropathy IgA dominant “Full house” C1q Renal Pathology The Osler Institute Review Course Immunofluorescence Microscopy Granular mesangial and capillary wall staining Membranous GN IgG,C3 MPGN C3 WHO Lupus III, IV Full house Post infectious GN Diffuse smudgy mesangial and capillary wall staining Amyloidosis lambda light chains Fibrillary GN IgG, C3, kappa > lambda MIDD kappa light chains Renal Pathology The Osler Institute Review Course Electron Microscopy Subepithelial immune complex type dense deposits Membranous GN Stage I - IV MPGN WHO Lupus IV Post infectious GN “Humps” Intramembranous (often with mesangial) immune complex type deposits Dense deposit Disease Renal Pathology The Osler Institute Review Course Electron Microscopy Subendothelial immune complex type deposits MPGN ISN/RPS Lupus III, IV Fingerprint, TRS Cryoglobulinemia Tubular substructure Pure mesangial immune complex type deposits IgAN / HSP ISN/RPS Lupus I, II C1q nephropathy Subendothelial immune complex type deposits ISN/RPS Lupus class III, IV MPGN Type III Postinfectious GN Renal Pathology The Osler Institute Review Course Clinical Patterns of Glomerular Disease Renal Pathology The Osler Institute Review Course Clinical Patterns of Glomerular Diseases Nephrotic Syndrome • Edema • Proteinuria > 3.5 gm/24 hours • Hypoalbuminemia • Hyperlipidemia • Hyerplipiduria Renal Pathology The Osler Institute Review Course Clinical Patterns of Glomerular Diseases Nephritic Syndrome • Hematuria • Red cell casts • Variable levels of proteinuria • Acute or chronic renal failure • Hypertension Renal Pathology The Osler Institute Review Course Classification of Glomerular Diseases • • Primary renal disease: renal limited injury Secondary renal disease: systemic disease which involves the kidney Nephrotic Syndrome Primary Minimal Change FSGS Membranous GN MPGN Secondary Diabetes Amyloid Nephritic Syndrome Primary Post Infectious GN Crescentic GN IgA/HSP Secondary SLE Vasculitis Renal Pathology The Osler Institute Review Course Nephrotic Syndrome Renal Pathology The Osler Institute Review Course Minimal Change Disease (Nil Disease, Lipoid Nephrosis) Clinical Features • • • • • • Peak age is 2 to 6 years Severe proteinuria, nephrotic syndrome Selective proteinuria Normal renal function Acute renal failure in adults Associated with exposure to allergens or immunizations • Associated with Hodgkin’s disease, NSAID therapy Renal Pathology The Osler Institute Review Course Minimal Change Disease (Nil Disease, Lipoid Nephrosis) Pathogenesis • • • • Immune disorder Circulating permeability factors Loss of fixed, GCW polyanionic charge Decreased and altered distribution of nephrin in podocytes Renal Pathology The Osler Institute Review Course Minimal Change Disease (Nil Disease, Lipoid Nephrosis) Light Microscopy • Normal glomeruli • Foam cells • Resorbtion droplets in the PCT Immunofluorescence Microscopy • No staining for G, A, M, C3, C1q, kappa, lambda Renal Pathology The Osler Institute Review Course Minimal Change Disease Electron Microscopy • Foot process effacement and microvillous transformation Prognosis • Remission in 8 weeks with steroid therapy • No tendency to progress to chronic renal disease Renal Pathology The Osler Institute Review Course Focal Segmental Glomerulosclerosis Clinical Features • • • • • • Heavy, non selective proteinuria Nephrotic Syndrome Microhematuria Hypertension Idiopathic FSGS Secondary FSGS HIVAN Heroin abuse Morbid obesity Unilateral renal agenesis Vesicoureteral reflux Renal Pathology The Osler Institute Review Course Focal Segmental Glomerulosclerosis Pathogenesis of FSGS • • • • • Hyperfiltration/hyperperfusion injury Circulating permeability factor Most cases are sporadic Few cases are associated with genetic abnormalities Viral infections - HIV, parvovirus B19 Renal Pathology The Osler Institute Review Course Focal Segmental Glomerulosclerosis Light Microscopy • • • • • • Juxta-medullary glomeruli Focal glomerular involvement Segmental glomerulosclerosis Foam cells Peripheral hyalinosis Tubular atrophy and interstitial inflammation Renal Pathology The Osler Institute Review Course Focal Segmental Glomerulosclerosis Columbia Classification • FSGS, NOS Typical course • Collapsing Type Poor prognosis • Tip Lesion ? Better prognosis • Perihilar variant Secondary FSGS Renal Pathology The Osler Institute Review Course Focal Segmental Glomerulosclerosis Immunofluorescence Microscopy • IgM and C3 in the sclerosed glomerular segments Electron Microscopy • Podocyte injury • Protein droplets in podocytes • Lamellar subepithelial reduplication of the glomerular basement membrane • Foot process effacement and microvillous transformation Renal Pathology The Osler Institute Review Course Focal Segmental Glomerulosclerosis HIV Associated Nephropathy • • • • • • • • HIV positive African American male Short clinical history Late manifestation of HIV infection Rapid progression to end stage renal disease Collapsing variant of FSGS Microcystic tubular dilatation Mononuclear interstitial inflammation Tubuloreticular structures in endothelial cells Renal Pathology The Osler Institute Review Course Membranous Glomerulonephritis Clinical Features • Most common cause of nephrotic syndrome in non-diabetic adults • Insidiuous onset of heavy proteinuria • Most common de novo glomerulonephritis in renal allografts Renal Pathology The Osler Institute Review Course Membranous Glomerulonephritis Secondary MGN Infection Hepatitis B, Hepatitis C, Syphilis, Parastic disease Drugs Mercury, Gold, penicillamine, NSAID, Catopril, formaldehye Neoplasms Renal carcinoma, lung, stomach, breast, Hematolymphoid neoplasms, pancreas, melanoma, seminoma Autoimmune Disease SLE, pemphigoid, MCTD, thyroiditis Others Diabetes, renal vein thrombosis, Sjogren’s, sarcoid, cryoglobulinemia, SCD Renal Pathology The Osler Institute Review Course Membranous Glomerulonephritis Light Microscopy • Normocellular glomerulus • Thickened glomerular basement membranes • Spikes and rings seen on Jones methenamine silver • Variable interstitial fibrosis • Interstitial foam cells may be present D’Agati Columbia-Presbyterian University Renal Pathology The Osler Institute Review Course Membranous Glomerulonephritis Immunofluorescence Microscopy • Granular IgG and C3 in the peripheral capillary wall Electron Microscopy • Supepithelial electron dense deposits • Subendothelial and mesangial deposits in secondary membranous GN • Thickened basement membrane • Foot process effacement and microvillous transformation Renal Pathology The Osler Institute Review Course Membranous Glomerulonephritis Ehrenreich and Churg - Morphologic Classification • Stage I Subepithelial electron dense deposits, normal GBM • Stage II Subepithelial electron dense deposits surrounded by cup-like extensions of the glomerular basement membranes • Stage III Subepithelial deposits, variable electron density, neomembranes are present on the surface of the deposit • Stage IV Irregularly thickened GBM and no deposits Renal Pathology The Osler Institute Review Course Membranous Glomerulonephritis Prognosis • Slow deterioration to renal failure • Corticosteroids and cytotoxic agents may be beneficial in idiopathic membranous GN • Secondary membranous GN may regress Renal Pathology The Osler Institute Review Course Membranoproliferative GN Type I Clinical Features • • • • • • • • • • • More common in children Uncommon in adults > 60 years of age Prodrome of an upper respiratory infection Hypertension Proteinuria - always present, moderate to heavy , poorly selective Hematuria - macroscopic hematuria is uncommon Nephritic Syndrome Nephrotic Syndrome Altered renal function - decrease GFR, Elevated BUN, Creatinine Hypocomplimentemia Circulating immune complexes (20%) Renal Pathology The Osler Institute Review Course Membranoproliferative GN Type I Glomeruli Enlarged, hypercellular tuft Lobular accentuation Infiltrating monocytes and neutrophils Crescents (2 - 10%) Thick peripheral glomerular membranes Silver stain - Double contours Tubules Hyaline (protein and lipid) droplets in tubular epithelium Tubular atrophy Interstitium Interstitial fibrosis, foam cells Iflammatory cells Vessels Arteriosclerosis Vasculitis (Cryoglobulinemia) Renal Pathology The Osler Institute Review Course MPGN Type II – Dense Deposit Disease Clinical Features • • • • • • • • • Children and young adults URI prodrome in ~50% of patients Proteinuria with hematuria Nephrotic syndrome is the most common presentation Acute nephritic syndrome (16 – 38%) Partial lipodystrophy C3 – low, fluctuating, normal C1q, C4 – normal C3NeF - IgG autoantibody against complement inhibitory proteins • Factor B, Properdin – low Renal Pathology The Osler Institute Review Course MPGN Type II – Dense Deposit Disease Pathology Glomeruli • Thick GBM • Hypercellular tuft • Leukocyte infiltration • Mesangial sclerosis Immunofluorescence • C3, IgM - segmental Electron Microscopy • Undulating ribbon-like deposits in the GBM, mesangium and tubular basement membranes Renal Pathology The Osler Institute Review Course Membranoproliferative GN Prognosis • Uniformly poor • Prognosis for Type II is generally worse than Type I • Response to alpha interferon - MPGN associated with Hepatitis C • Type II MPGN has 90% recurrence rate in allograft kidney Renal Pathology The Osler Institute Review Course Glomerular Diseases with a Membranoproliferative Pattern • • • • • • • • Diabetic glomerulosclerosis Cryoglobulinemic Glomerulonephritis Systemic Lupus nephritis (WHO Class IV) Fibrillary glomerulonephritis Hepatitis viral infection Chronic liver disease Light chain deposition disease Lecithin-cholesterol acyltransferase deficiency Renal Pathology The Osler Institute Review Course Diabetic Nephropathy Clinical Features • Ten year or more history of diabetes mellitus • Microalbuminuria is an early clinical feature • Type I diabetes mellitus has good correlation between renal disease and severity of fundoscopic findings • Hypertension is common Renal Pathology The Osler Institute Review Course Diabetic Nephropathy Etiology and Pathogenesis • Irreversible glycosylation of the glomerular basement membranes • Growth factors • Genetic susceptibility • Glomerular hyperperfusion or hyperfiltation injury Renal Pathology The Osler Institute Review Course Diabetic Nephropathy Light Microscopy • Diffuse increase in mesangial matrix • Sclerotic mesangial nodules • Fibrin cap (peripheral hyalinosis) • Capsular drop • Hyaline arteriolosclerosis of the afferent and efferent arterioles • Arteriosclerosis • Protein droplets in tubular epithelium • Glycogen in tubules - Armani Ebstein anomaly • Pyelonephritis • Papillary necrosis Renal Pathology The Osler Institute Review Course Diabetic Nephropathy Immunofluorescence Microscopy • IgG - low intensity linear accentuation of the glomerular basement membrane • Albumin - low intensity linear accentuation of the glomerular basement membrane • IgM and C3 in sclerosed glomerular segments Electron Microscopy • Podocytes show foot process effacement • Uniformly thick glomerular basement membrane • Expanded mesangial matrix Renal Pathology The Osler Institute Review Course Diabetic Nephropathy Prognosis • Progression to end stage renal failure ranges from 3 to 20 years • Renal failure 40% of Type I diabetics 20% Type II diabetics • Recurs in allograft kidney • Control of hyperglycemia and hypertension delays the progression of diabetic nephropathy Renal Pathology The Osler Institute Review Course Amyloidosis Clinical Features • AL Type amyloid - Plasma cell dyscrasia Most common is AL monoclonal light chain, l > k Overt myeloma is present in 10 – 20% • AA Type amyloid - Chronic inflammatory diseases Osteomyelitis, TB, leprosy, rheumatoid arthritis • Neoplasms - Renal cell carcinoma • Familial Mediterranean Fever • Severe proteinuria • Nephrotic syndrome Renal Pathology The Osler Institute Review Course Amyloidosis Light Microscopy • Smudgy, homogenous deposits in glomeruli, tubular basement membranes, peritubular capillaries and blood vessels • Deposits are weakly PAS positive and silver negative • Detection: Congo Red, antibody to AA, Thioflavine T
