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Mr. Clark Kent, Dr. Pamela Smith, & Dr. Leroy Jenkins
Doctoral Program in Educational Leadership
Texas A&M University-Corpus Christi College of Education and Human Development
Abstract
Methods
Malaria is an infectious disease spread by the female Anopheles mosquito A
quick summary of the research A quick summary of the research A quick summary
of the research A quick summary of the research A quick summary of the research
A quick summary of the research A quick summary of the research A quick
summary of the research A quick summary of the research A quick summary of the
research A quick summary of the research A quick summary of the research A
quick summary of the research A quick summary of the research A quick summary
of the research A quick summary
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Introduction
Blah blah blah is a parasite-induced disease that is a significant medical and
economical burden due to the introduction of the research the introduction of the
research the introduction stuff introduction stuff introduction stuff introduction stuff
introdhttp://education.tamucc.edu/resources/index.htmluction stuff introduction stuff
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Conclusion
Results
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Reference list goes here Reference list goes here
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Reference list goes here
The protozoan P. falciparum has a very complicated lifecycle consisting of threestages. More introduction stuff maybe,
Steps towards the Edible
Malaria Vaccine
the host More introduction stuff
maybe, More introduction stuff
maybe, More introduction stuff
maybe, MFor future research once all
genes have been sub-cloned into the
pSAT vector they will then be used to
transform carrot calli. The transgenic
plants will then co-express the gene
products in the carrot plants.
The lifecycle proteins that are currently being targeted include MSP-1_19, AMA1,
and MSP2, all from the blood stage, Pfs48_45 from the sexual stage, and SSP2
from the liver stage. Wow this is complicated is anyone actually reading this. The
main focus of this research, though, is to isolate MSP1_19 and insert it into the
pGEM-T easy vector as well as a plant expression referred to as the pSAT vector.
For future research once all genes have been sub-cloned into the pSAT vector
they will then be used to transform carrot calli. The transgenic plants will then coexpress the gene products in the carrot plants.
Isolation of a surface
protein within the
blood stage
Isolation of a surface
protein within the
sexual stage
Isolation of a surface
protein within the
liver stage
MSP-1_19
Pfs48_45
SSP2
Insert target genes into plant
expression vector pSAT.
The pSAT vector can
contain up to 5
expression cassettes.
Each cassette contains
autofluorecsent
proteins for
identification
purposes.
the host More introduction
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More
introduction
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maybe,
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introduction
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maybe, MFor future research
once all genes have been.
p - SAT
Using Agrobacterium tumefaciens transform
carrot calli
Verify gene expression using
western blots
Test that the appropriate
antigens are being produced
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References
Blah blah blah is a parasite-induced disease that is a significant medical and
economical burden due to the introduction of the research the introduction of the
research the introduction stuff introduction stuff introduction stuff introduction stuff
introdhttp://education.tamucc.edu/resources/index.htmluction stuff introduction stuff
introduction stuff introduction stuff introduction stuff introduction stuff introduction
stuff introduction stuff introduction stuff introduction stuff introduction stuff
introduction stuff introduction stuff introduction stuff introduction stuff introduction
stuff introduction stuff introduction stuff
the in