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Midterm Examination 2 Review Sheet Section I: Metabolism and Sugar Catabolism What is metabolism? What is catabolism and anabolism? How do these two processes relate to metabolism? What is an enzyme, how does the enzyme change a chemical reaction? How does ATP and ADP function? What is an active site? What is a kinase? What is glycolysis? Where does it occure in a prokaryotic versus eukaryotic cell? What is the chemical formula for glucose? C6H12O6 Why does glycolysis start with a sugar like glucose? What is so special about glucose in terms of energy production? How can sugars besides glucose be used in sugar catabolism? What is the first process in glucose catabolism? Excitation of the glucose molecule by the addition of phosphates from ATP input. What does this excitation do? Breaks apart glucose to 3-Carbon molecules. What is the point of NAD’s in aerobic versus anaerobic metabolism? How much ATP is produced in glycolysis, gross versus net? What is the point of fermentation during anaerobic metabolism? When is it used during aerobic metabolism? What is the point of putting a CoA onto acetyl? What are the molecules that form citric acid during the Kreb’s cycle? What are the products of the Kreb’s cycle? How do you change a 3-Carbon molecules to a 2-C molecule? What is the point of the electron transport chain? What are the two forces that drive the flow of H’s back through the ATP Synthetase machinery? What is ATP Synthetase? Where does the Kreb’s cycle occur in a prokaryotic versus eukaryotic cell? Where does the ATP Synthetase work in a prokaryotic versus eukaryotic cell? What is a phenol red test? How does it tell you what metabolism is occurring? What is the difference between catabolism, anabolism, metabolism, aerobic, anaerobic? What is the purpose of glycolysis, fermentation, the Citric Acid Cycle, the electron transport chain and ATP synthetase? What is the purpose of the mitochondria? What processes happen in the different parts? Example questions: Glycolysis is the catabolism of glucose so: Glucose is (A) Built up, (B) Kept the same, (C) Broken down, (D) None of the above Glycolysis is the catabolism of glucose so: Energy is (A) Stored, (B) Kept the same, (C) Released, (D) None of the above Glycolysis is (A) Aerobic, (B) Anaerobic, (C) None of the above Lactic acid fermentation is (A) Aerobic, (B) Anaerobic, (C) None of the above Ethanol fermentation is (A) Aerobic, (B) Anaerobic, (C) None of the above Preparation for respiration is (A) Aerobic, (B) Anaerobic, (C) None of the above Kreb’s cycle is (A) Aerobic, (B) Anaerobic, (C) None of the above Electron Transport Chain is (A) Aerobic, (B) Anaerobic, (C) None of the above ATP Synthetase is (A) Aerobic, (B) Anaerobic, (C) None of the above The majority of anaerobic metabolism occurs in the (A) Nucleus, (B) Cytoplasm, (C) Mitochondria, (D) None of the above The majority of aerobic metabolism occurs in the (A) Nucleus, (B) Cytoplasm, (C) Mitochondria, (D) None of the above Page 1 of 2 Midterm Examination 2 Review Sheet How many ATPs are consumed in glycolysis per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above How many net ATPs are produced in glycolysis per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above How many gross ATPs are produced in glycolysis per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above How many net ATPs are produced in fermentation per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above How many net ATPs are produced in electron transport chain per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above How many net ATPs are produced in Kreb’s cycle per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above How many net ATPs are produced in ATP Synthetase per glucose? (A) 2, (B) 4, (C) 6, (D) 1, (E) None of the above Where is NAD replenished in aerobic metabolism? (A) Glycolysis, (B) Fermentation, (C) Kreb’s Cycle, (D) Electron Transport Chain, (E) None of the above Where is NAD replenished in anaerobic metabolism? (A) Glycolysis, (B) Fermentation, (C) Kreb’s Cycle, (D) Electron Transport Chain, (E) None of the above What is the equivalent of NADH+H? (A) GTP, (B) CO2, (C) H2O, (D) FADH2, (E) None of the above What is the equivalent of ATP? (A) GTP, (B) CO2, (C) H2O, (D) FADH2, (E) None of the above Using ATP to make a cell process occur is the ____?____ of ATP. (A) Catabolism, (B) Anabolism (C) Aerobic, (D) None of the above Creating ATP in glycolsis is the ____?____ of ATP. (A) Catabolism, (B) Anabolism (C) Aerobic, (D) None of the above Creating ATP in ATP Synthetase is the ____?____ of ATP. (A) Catabolism, (B) Anabolism (C) Aerobic, (D) None of the above The gradient of aerobic respiration are caused by H+’s moving to the __?__ of the mitochonidria. (A) Matrix, (B) Lumen, (C) Cytoplasm, (D) None of the above The Kreb’s cycle occurs in the __?__ of the mitochonidria. (A) Matrix, (B) Lumen, (C) Cytoplasm, (D) None of the above The Electron transport chain occurs in the __?__ of the mitochonidria. (A) Matrix, (B) Lumen, (C) Cytoplasm, (D) None of the above Overall, the electron transport chain moves __?__ completely across the inner membrane. (A) electrons, (B) hydrogen ions, (C) water, (D) oxygen, (E) None of the above Water formation in ATP Synthetase occurs in the __?__ of the mitochonidria. (A) Matrix, (B) Lumen, (C) Cytoplasm, (D) None of the above During ATP Synthetase, Hydrogren ions bind to __?__ to keep the gradients intact. (A) CO2, (B) Glycolysis, (C) oxygen, (D) None of the above Page 2 of 2