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Why do cells divide? Mar 2310:39 AM Questions • Read pages 241 to 243. • Answer the questions on the worksheet. • On a separate piece of paper: 1. Explain in detail the two reasons why cells divide. 2. Explain how the function of a chloroplast and a mitchondria may be impaired if a cell were to become too large. Mar 221:57 PM Mar 2310:32 AM Mar 2310:32 AM Mar 2310:33 AM The Cell Cycle http://www.youtube.com/watch?v=4W8kupKFCPk Mar 221:57 PM The Cell Cycle • During the cell cycle, a cell grows, prepares for division, and divides to form two daughter cells. These two daughter cells will begin the cycle again. • Consists of 4 phases: G1 phase S phase G2 phase M phase Mar 221:57 PM The Cell Cycle M phase Mar 221:57 PM Interphase • Longest event in a cell’s life. • Period of great metabolic activity. • When a cell can no longer maintain homeostasis it must divide. G1 phase: The cell increases in size and synthesize new proteins and organelles. S phase: DNA replication. G2 phase: Various organelles and molecules required for cell division are created at this time. Mar 221:57 PM DNA Replication: (S-Phase) "You are what you eat" ???? An enzyme called DNA Helicase causes the base pairs of DNA to split down the middle forming 2 single strands. The nucleus contains free floating nucleotides from the food you eat. These nucleotides are attached to each single strand of DNA by another enzyme DNA Polymerase to form 2 identical double stranded DNA molecules. Mar 221:57 PM A T T A C G Helicase Mar 221:57 PM A T T A C G Mar 221:57 PM A T T A C G Draw a diagram to represent the replication of a DNA molecule with the letters A,C,T down the left hand side. step 1: show original molecule step 2: show split molecule step 3: (in a different colour) fill in your missing nucleotides to your drawing from step 2 Mar 2510:34 PM Interphase Mar 221:57 PM M phase: 1. Mitosis (division of the nucleus) 2. Cytokinesis (division of the cytoplasm) Mar 2510:32 PM What is Mitosis? Mitosis is the division of the cell nucleus. There are 4 main events or phases make up mitosis: Prophase Metaphase Anaphase Telophase Mar 221:57 PM Prophase • Replicated chromatin from interphase begin to shorten , thicken , and coil up to form Chromatid Pairs. • Nucleolus & nuclear envelope disappears. • Centrioles split and move to opposite sides of the cell (moves to the poles). Protein fibers begin to radiate from each. These structures are called Asters. • When asters reach opposite sides of cell they form a Spindle. Mar 221:57 PM Prophase • Note: Plant Cells do not have centrioles or asters but the spindles still form Mar 221:57 PM Metaphase • Shortest stage of mitosis. • Chromatid pairs align at the equator of the spindle. Mar 221:57 PM Anaphase • Centromere splits and chromatid pairs separate. • Spindle fibers begin to contract and drag the Chromosomes to opposite sides of the cell. Mar 221:57 PM Telophase Mar 221:57 PM Telophase • Final stage of mitosis , chromosomes reach the poles and begin to uncoil to form chromatin. • Spindle fibers disappear , nuclear envelope & nucleolus begin to reappear. • In early telophase the cell membrane begins to indent at the equator to form a Cleavage Furrow (start of actual cytoplasm division called Cytokinesis). • In late telophase cleavage furrows have indented further. • MITOSIS IS NOW OVER !! Mar 221:57 PM Mar 2710:58 AM Cytokinesis • Refers to the division of the cytoplasm to produce 2 genetically identical daughter cells. • Animal & Plant cell’s achieve cytokinesis differently. Mar 221:57 PM Animal Cell • Animal cell divides from the outside in by forming these cleavage furrows. The cleavage furrows eventually pinches off the middle. Mar 221:57 PM Plant Cell Cytokinesis • Plant cells divide from inside out through the formation of a Cell Plate. Mar 221:57 PM Mitosis Animation http://phschool.com/webcodes10/index.cfm ?wcprefix=cbp&wcsuffix=3102 &area=view&x=0&y=0 Mar 271:01 PM INTERPHASE (G1, S, G2) MPHASE (Mitosis) Prophase Metaphase Anaphase Telophase (Cytokinesis) Mar 2711:46 AM Mar 273:20 PM