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Chapter 6 : Chromosomes and Cell Reproduction Section 3: Mitosis & Cytokinesis Chromatid Separation in Mitosis • During mitosis the chromatids on each chromosome are physically moved to opposite sides of the dividing cell with the help of the spindle. • Spindles are cell structures made up of both centrioles and individual microtubule fibers that are involved in moving chromosomes during cell division. Forming the Spindle • When a cell enters the mitotic phase, the centriole pairs start to separate, moving toward opposite poles of the cell. • As the centrioles move apart, the spindle begins to form. Separation of Chromatids by Attaching Spindle Fibers • The chromatids are moved to each pole of the cell in a manner similar to bringing a fish in on a fishing rod and reel. • When the microtubule “fishing line” is “reeled in,” the chromatids are pulled to opposite poles. • As soon as the chromatids separate from each other they are called chromosomes. Mitosis •Step 1: Prophase • Chromosomes condense and become visible. The nuclear envelope dissolves and a spindle forms. Mitosis (continued) • Step 2: Metaphase • The chromosomes move to the center of the cell and line up along the equator. Spindle fibers link the chromatids of each chromosome to opposite poles. *longest phase of mitosis! Mitosis (continued) • Step 3: Anaphase • Centromeres divide, and two chromatids (now called chromosomes) move toward opposite poles as spindle fibers attached to them begin to shorten. • “Ana” means “back” or “backward” Mitosis (continued) • Step 4: Telophase • A nuclear envelope forms around the chromosomes at each pole. • Chromosomes, now at opposite poles of the cell, uncoil and the spindle dissolves. Spindle fibers break down and disappear. MITOSIS IS NOW COMPLETE Cytokinesis • As mitosis ends, cytokinesis begins. • During cytokinesis, the cytoplasm of the cell is divided in half and the cell membrane grows to enclose each cell, forming two separate cells as a result. • The end result of mitosis and cytokinesis is two genetically identical cells, where only one cell existed before. Cytokinesis: Animal Cells vs. Plant Cells Animal Cells • Lack a cell wall Plant Cells • Vesicles formed by the golgi apparatus fuse at the midline of the dividing cell and form a cell plate across the middle of a plant cell. • Cell is pinched in half by a belt • A new cell wall forms on both of protein threads, forming two sides of the plate and separates separate animal cells. into two plant cells. MITOSIS EXIT SLIP • Draw each step of mitosis and describe what is happening during each step! • You can use your notes! • Place in inbox before you leave. • Make sure your NAME, PERIOD, AND DATE are at the top of your paper. Thanks!