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ERT 445
FINAL YEAR PROJECT 1
METHODOLOGY
Dr. Syazni Zainul Kamal
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INTRODUCTION
•Now you are actually talking about what
you are proposing.
•be sure that this follows naturally from
the Introduction in which you should
have set up and highlighted some critical
issue that needs to be resolved.
•tell the reader how you would resolve
this issue.
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PURPOSE
•This section will provides a written
description of the specific actions, plan, or
strategy you will take to answer your
research questions.
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METHODOLOGY
•HOW YOU INVESTIGATE THE QUESTION?
•WHAT WILL YOU DO?
•HOW YOU WILL DO IT?
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CONTENTS
•This section should describe the proposed
method for the experiment, including
•Research Framework (Overall Research Work)
•List of Equipments and Materials (that will be
used)
•Procedure (that will be followed)
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RESEARCH FRAMEWORK/FLOW
CHART
•Overall view of steps/flow in your
research
•Use mind map
•Must have continuous flow
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Sampling Bacteria Source
2
Pre - Treatment
1
Example
Testing Using RCM Medium in
Serum Bottle
Evaluation
No
H2 Potential
Yes
Active Inoculum
Dark Fermentation
Stock Culture
Optimization Studies
Inoculum Development
pH
Characterization of Agriculture Waste
Kinetic Study
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Equipment and materials
•In this subsection of the method section you
describe
any
equipment
and
materials/chemicals in your research study.
•The following information should be
included:
• General description of the apparatus or
instruments.
• Reference indicating where equiments
can be obtained.
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Material
Brand
2,2-diphenyl-1-picrylhydrazyl
Sigma, Malaysia
Ethanol
Merck, Malaysia
Ethyl acetate
Merck, Malaysia
Acetone
Sigma, Malaysia
Equipment
Location
Beaker
School of Bioprocess, UniMAP
Test tube
School of Bioprocess, UniMAP
Incubator
School of Bioprocess, UniMAP
Grinder
School of Bioprocess, UniMAP
UV-VIS Spectrophotometer
School of Bioprocess, UniMAP
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PROCEDURE
•In this subsection of the methodology section
of your proposal, you carefully describe how
your study will be executed.
•The following information should be included
in the procedure section:
• A description of the design if it was not
previously described.
• A detailed step-by-step description of how
the study will be executed.
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PROCEDURE
list down all scopes with details description
-material/sample preparation, design work,
simulation/modelling work, development or
fabrication work, characterization and
optimization
-should specify the variables/parameters
(independent and dependent) that will be
used in the experiment with the min and max
range that will be run by DOE
-Determine how many of replicate / control
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5.3 Procedure
5.3.1 Plant collection and sample preparation
The plant of Stachytarpheta indica will be collected from
Kampung Wang Tepus, Jitra, Kedah. The whole plant collected will
be washed thoroughly under running tap water to remove the
dust and soil. Then, the plant will undergo drying process by using
oven at 50°C overnight.
5.3.2 Plant extraction process
Explain in detail the process
5.3.3 Antibacterial assay
Explain in detail the process
5.3.4 Phytochemical analysis
Explain in detail the process
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RUBRIC
NO
1
2
3
4
5
CRITERIA
EXEMPLARY (4)
PROFICIENT (3)
ACCEPTABLE (2)
UNACCEPTABLE
(1)
Rational of
-All techniques used
-All techniques used
-Some techniques
-Some techniques
Techniques Used
during the experiment during the experiment
used during the
used during the
very well explained.
were explained.
experiment were not experiment were
explained clearly.
not explained.
Understanding of
-Demonstrate an in-Demonstrate an
-Demonstrate an
-Unable to
Concept and
depth understanding of understanding of
inadequate
demonstrate
Principle
concept and principles concept and principles
understanding of
understanding of
of each method.
of each method.
concept and
methodology
principles of each
concept.
method.
Sequence
-Actions well
-Actions presented in a
-Some of the
-Actions were not
presented in a logical
logical sequence.
actions are not
presented in a
sequence.
presented in a
logical sequence.
logical sequence.
Materials
-All the materials used -The materials used
-Some of the
-None of the
were included and
were included generally. materials used were materials used
clearly described.
included.
were included.
Grammar and
-No errors in spelling
-Seldom errors in
-Frequent errors in
-Abundance of
Sentence Structure and grammar.
spelling and grammar.
spelling and
errors in spelling
Appropriate tenses
Appropriate tenses were grammar.
and grammar.
were used.
used.
Appropriate tenses
No appropriate
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were used partly.
tenses were used.
EXPECTED RESULT
WHAT DO YOU EXPECT TO FIND?
Based on the present researches, the zone of
inhibition obtained in the disc diffusion test should
be more than 14mm to shown the plant extract has
the ability to inhibit the bacteria growth. The MIC
obtained should be less than 1000ug/ml in order for
the plant extract to possess antibacterial activities.
The plant extract should give a stronger antibacterial
effect to gram-positive bacteria than gram-negative
bacteria.
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Any
questions?
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NEW DATE OF SUBMISSION
Progress report submission due date
Introduction, Problem statement, research
objective, literature review, references of
proposal report
(2 copies)
Methodology (2 copies)
30 Sept 2016
Finalized proposal report (4 copies)
19 Oct 2016
Proposal defense
(slide presentation)
31Oct-4Nov 2016
(after mid sem
break)
Corrected proposal (2 copies)
11Nov 2016
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14 Oct 2016
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