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10.5005/jp-journals-10021-1192 ORIGINAL ARTICLE Puneet Yadav et al Evaluation and Comparison of the Color Stability of Various Esthetic Brackets, When Exposed to Various Food Dyes: An in vitro Study 1 Puneet Yadav, 2Pushpa Vinay Hazarey, 3Seema Grover, 4Maninder Sidhu, 5Vikas Malik ABSTRACT Aim: Due to an increased demand for better esthetic during orthodontic treatment, tooth colored brackets were introduced. Esthetic brackets can be either ceramic or composite, but their color stability remains main concern for clinicians and patients. The present in vitro study was conducted to investigate, evaluate and compare color stability of various esthetic brackets when exposed to various food dyes. Further, effect of time on color stability of esthetic brackets was evaluated. Materials and methods: Total 120 upper central incisor brackets were taken for study. They were divided into three Groups of 40 brackets each. Group I, ceramic brackets of American orthodontics, Group II, ceramic brackets of 3M Unitek and Group III composite brackets of Libral traders. Frequently used beverages; drinking water, tea, coffee, coke were used in study. For control value, color of all brackets at 0 day was noted. Then brackets of all groups were immersed in different solutions for 24 (1 day), 72 (3 days), and 144 hours (6 days) respectively and compared with control value. Results: All brackets showed significant color changes on sixth day. Group I showed 'E value of 5.18 for coffee, 5.17 for tea indicating more staining with coffee than tea. Group II showed 'E-value of 5.05 for tea, 4.16 for coffee suggesting more color variation with tea than coffee. Group III 'E values were 6.11 for coffee, 8.63 for tea. Hence, color variations were more prominent with tea than coffee in all groups. Coke and water discolorations were least in all groups on sixth day. Keywords: Esthetic, Bracket, Dyes. How to cite this article: Yadav P, Hazarey PV, Grover S, Sidhu M, Malik V. Evaluation and Comparison of the Color Stability of Various Esthetic Brackets When Exposed to Various Food Dyes: An in vitro Study. J Ind Orthod Soc 2013;47(4):382-389. INTRODUCTION The growing population of adult orthodontic patients not only wants an improved smile, but also better esthetics during the treatment. Over the years, the esthetic appearance of fixed orthodontic appliance has become a vital concern. As the number of adults seeking orthodontic treatment increased, the esthetic brackets were introduced.1 Three types of orthodontic bracket are currently available; metal, ceramic and plastic. The metal brackets although provide good mechanical properties, esthetically they are not appreciated.2 Although various modalities have been incorporated in orthodontics to improve esthetics during the treatment, but 1,5 Senior Lecturer, 2,4Professor and Head, 3Professor Department of Orthodontics, SGT Dental College, Gurgaon Haryana, India 2 Department of Orthodontics, Sharad Pawar Dental College, Wardha Maharashtra, India 1,3-5 Corresponding Author: Puneet Yadav, Senior Lecturer, Department of Orthodontics, SGT Dental College, Gurgaon, Haryana, India e-mail: [email protected] Received on: 3/3/12 Accepted after Revision: 27/6/12 382 composite and ceramic brackets remain the most popular options preferred by the patients. Composite bracket are made up of polycarbonate which adsorbed water during orthodontic treatment. In the mid 1980s, first ceramic bracket made of monocrystalline and polycrystalline materials became widely available.3 An increased strength is a major advantage of ceramic brackets over composite brackets. Nevertheless, the use of ceramic brackets may result in problems with excessive bond strength and damage the enamel during removal due to their brittle nature.4 The color stability of these esthetic brackets has remained the main concern for clinicians as well as patients. Though, the ceramic brackets had similar disadvantages of getting stained in the oral environment over a period of time, they were more resistant to various stresses and torquing forces. There are two types of discoloration of esthetic brackets: Internal (endogenous) and external (exogenous).5 The external discoloration is chiefly due to color dyes, such as food dyes, tea stains, coffee, colored mouth rinses, etc.6 The material, structure, filler content and surface roughness plays a decisive role in the extent of external discoloration.7 The chief culprit for internal discoloration was found to be UV radiations and thermal energy.8,9 JIOS Evaluation and Comparison of the Color Stability of Various Esthetic Brackets, When Exposed to Various Food Dyes Two general methods can be used to analyze the color of an object; visual and instrumental. Visual color determination is based on visual comparisons of the object with standard color. This method is most frequently applied in dentistry.10 Visual color assessments are a result of physiological and psychological responses to radiant energy stimulation. Alteration in perception can occur as a result of uncontrolled factors, such as fatigue, aging, emotions, lighting conditions and metamerism.11 It is the most scientific and practical method to assess color stability.12 Colorimetry is the branch of science concerned with numerically specifying the perceived color of the object as well as differences in perceived color between two objects judged to be different. 11 Reflected color is calculated according to Commission Internationale de l’ Eclairage (CIE) LAB color scale by measuring the ratio of reflected light to incident light (spectral reflectance) under specified geometric conditions. One of the most important features of CIELAB system is its arrangement as an approximately uniform three dimensional color space.13 The amount of color change can be influenced by number of factors, including oral hygiene, water sorption, incomplete polymerization and surface roughness. The purpose of the present in vitro study was to investigate the influence of various food dyes on ceramic brackets, and to evaluate and compare the color stability of various esthetic brackets, when exposed to various color dyes. Further effect of time on color stability of esthetic brackets was also evaluated. The samples were analyzed according to CIELAB color scale and the exact color changes on their surfaces were determined and compared with other experimental subgroups. MATERIALS AND METHODS A total of 120 upper right central incisors esthetic brackets were taken for the study. These brackets were divided into three groups according to their manufacture name. The ceramic brackets were included in this study were polycrystalline in nature. • Group I: 40 ceramic brackets of American orthodontics • Group II: 40 ceramic brackets of 3M Unitek • Group III: 40 composite brackets of Libral Traders Pvt. Ltd. Solutions: Four solutions were chosen as they are the most frequently used beverages in the daily life. 1. Packaged drinking water (Bisleri) 2. Tea (Lipton) 3. Coffee (Nescafe) 4. Cold drink (Coca Cola) Distilled water used was Diet Aqua, India. These groups were subdivided into following subgroups: Total 120 samples Drinking Tea Coffee Cold water (10 no.) (10 no.) drink (10 no.) (10 no.) 40 ceramic brackets (Group I) 40 ceramic brackets (Group II) 40 composite brackets (Group III) Ia Ib Ic Id IIa IIb IIc IId IIIa IIIb IIIc IIId For the control value, or the base value, each bracket group was analyzed for its original color value at 0 day interval, before its immersion into the solution. The values were recorded and were taken as the standard values for the further comparison with the experimental groups. Method for Analyzing the Samples Three customized porcelain stands, with desired slot size of each bracket type were fabricated so as to accurately fit the brackets in them. This was essential for error free readings as no external light sources could underpass the stand and thus interfere with the analysis and the readings. Each subgroup was then dipped in their respective solutions and were analyzed for the color changes at the time intervals of 1 day (24 hours), 3 days (72 hours) and 6 days (144 hours) respectively. For analyzing the samples, at the determined time interval, the brackets from each subgroup were taken out of the solution and rinsed for 20 minutes with deionized water (distilled water) to remove the excessive stains. It was then gently dabbed by filter paper to dry them. This method was followed for each subgroup in the study. The color measurements were carried out using the Spectrolino™ Spectrophotometer (Figure 1 Spectrolino™ Spectrophotometer GretagMacbeth TM ) with a pinhole diaphragm diameter of 4 mm according to the CIE L * a * b * system (Commission Internationale de l’Eclairage, 1976). Next, the brackets were placed in the slots of customized porcelain stands and were placed under the spectrophotometer. A color graph consisting of L *, a * and b* coordinates can be produced by means of mathematical transformations. In the CIELAB color space, L* is a measure of the lightness of an object and is quantified on a scale such that perfect black has an L* value of zero and a perfect reflecting diffuser an L* value of 100. The CIE a* value is a measure of redness or greenness, and b* is a measure of yellowness or blueness. As a* becomes more positive in value, the color is more red; as a* becomes more negative in value, the color becomes more green. As b* becomes more positive in value, the color becomes more yellow; as b* becomes more negative in value, the color The Journal of Indian Orthodontic Society, October-December 2013;47(4):382-389 383 Puneet Yadav et al becomes more blue. Absolute measurements can be made in L*a*b* coordinates and color change calculated as 'E (L*a*b*). 'E value of 3.7 or less is considered to be clinically acceptable. The formula used for calculating the value of the change in color is '( >'L'a)2 + ('b)2]1/2 The obtained values were then subjected to statistical analysis. RESULTS A total sample comprising of 120 brackets were used in this study. Groups I and II consisted of 40 ceramic brackets. Group III consisted of 40 composite brackets. These groups were divided into subgroups as per the solution in which they were immersed. The color measurements were carried out using the Spectrophotometer according to CIE L*a*b* color system. The results and the statistic values are enumerated in the form of tables. Table 1 shows comparison between base values for ceramic bracket Group I with that of subgroup Ia (drinking water), Ib (tea), Ic (coffee) and Id (coke) at first, third, and sixth day. On comparing Group I with Ia, there was a statistically significant change in the color of brackets when compared to their base values, this variation was most significant on the sixth day and the color changes on first and third day were not significant. However, the variation in the color change was not relevant clinically as the base value for the sixth day was 2.24, which is clinically acceptable. The change in color is clinically perceptible only when equal to, or above 3.5 to 3.7.14 When compared with Ib and Ic on third and sixth day, “E value was higher for coffee (4.54 and 5.18) than tea (4.96 and 5.17) showing more stains with coffee when used in Group I brackets (Fig. 2). Table 2 shows comparison between base values for ceramic brackets of Group II with that of subgroup IIa, IIb, IIc and IId. When compared with IIa (drinking water) at first, third and sixth day. It showed that the variation in color on sixth day was significant when compared to base value. When Group II was compared with subgroup IIb (tea) at first, third and sixth day, statistic analysis revealed significant variation in colors for all the three time intervals as compared to the control (base) values. However, highest value was seen on sixth day as 5.05. Similar findings were observed with subgroup IIc Table 1: Comparison of base values of Group I with its subgroups Ia, Ib, Ic, Id on first, third and sixth day respectively Group I No. sample Mean E Base value 1st day 3rd day 6th day – 10 10 10 2.20 1.49 1.79 2.24 0.82 1.31 0.32 0.51 0.26 0.41 0.10 0.16 – 0.89 0.13 2.13 – 0.384 0.895 0.032 Group Ib 1st day 3rd day 6th day 10 10 10 4.31 4.96 5.17 1.03 1.64 0.56 0.32 0.51 0.17 5.02 4.74 9.38 0.000 0.000 0.000 Group Ic 1st day 3rd day 6th day 10 10 10 2.68 4.54 5.18 0.78 0.42 1.39 0.24 0.13 0.44 1.33 15.20 5.80 0.199 0.000 0.000 Group Id 1st day 3rd day 6th day 10 10 10 3.09 3.51 3.51 0.76 0.49 0.49 0.24 0.15 0.15 2.49 4.27 4.27 0.022 0.000 0.000 Group Ia Std. deviation Std. error mean T p-value p < 0.05, Significant Table 2: Comparison of base values of Group II with its subgroups IIa, IIb, IIc, IId on first, third and sixth day respectively Group II No. sample Mean E Std. deviation Std. error mean T p-value Base value – 1.81 0.60 0.19 – – Group IIa 1st day 3rd day 6th day 10 10 10 1.13 1.46 1.74 0.83 0.88 0.39 0.26 0.27 0.12 1.07 0.22 3.00 0.295 0.823 0.008 Group IIb 1st day 3rd day 6th day 10 10 10 3.08 3.59 5.05 0.46 0.77 0.62 0.14 0.24 0.19 5.27 5.71 11.81 0.000 0.000 0.000 Group IIc 1st day 3rd day 6th day 10 10 10 3.61 3.63 4.16 1.02 0.55 0.58 0.17 0.32 0.18 4.81 6.92 8.81 0.000 0.000 0.000 Group IId 1st day 3rd day 6th day 10 10 10 1.98 2.26 2.46 0.87 0.43 0.47 0.27 0.13 0.14 1.921 0.689 1.647 0.071 0.499 0.117 p < 0.05, Significant 384 JIOS Evaluation and Comparison of the Color Stability of Various Esthetic Brackets, When Exposed to Various Food Dyes Fig. 1: Spectrolino™ Spectrophotometer Gretag MacbethTM Fig. 2: Comparison of base values with Subgroups of Ia, Ib, Ic and Id at first, third and sixth days Fig. 3: Comparison of base values of Group II with subgroups IIa, IIb, IIc and IId at first, third and sixth days (coffee), where highest value observed on sixth day was 4.16 and least on first day being 3.61. Whereas no statistically significant color variation was observed on any day with subgroup IId (coke). The maximum “E for coke subgroup was 2.46 (Fig. 3). Fig. 4: Comparison of base values of Group III with subgroups IIIa, IIIb, IIIc and IIId at first, third and sixth days Table 3 shows comparison of composite brackets base values Group III with subgroup IIIa, IIIb, IIIc and IIId on first, third and sixth day. When compared with IIIa (drinking water), statistically significant color change was observed on third and sixth day. The E value for this day was 3.05, hence, color change was clinically acceptable. Tea and coffee showed highest values 8.63 and 6.11 on sixth day respectively (Fig. 4). Table 4 shows the statistical intragroup comparison of Group I when immersed in various solutionsat day 1. Although color change was clinically insignificant for all the subgroups analyzed, color variation was significantly noticeable between subgroups Ia and Ib having value of –2.54, between Ia and Id, value of E being –1.32. However, the color variation between subgroups Ia, Ic and Id were not significant having value –0.92 and –0.40 respectively. On sixth day, statistically significant variation in color change was observed between subgroups Ib and Id. The mean difference was found to be maximum between the Groups Ia, Ib which was –3.68 followed by Groups Ia and Id which was –2.01. Table 5 shows the comparison in between subgroups of Group II when immersed in various solutions on 1st, 3rd and sixth day. On first day, maximum statistical variation in the color change was seen between subgroups IIa, IIc which was of value –2.16 followed by subgroups IIa, IIb having a value of –1.61. On 3rd day, statistically significant color variation was noticed between subgroups IIa, IIc (–1.87) followed by subgroups IIa, IIb (–1.85). This suggests that color variation was higher in coffee than tea. However, the variations in the color stability were insignificant between the Groups IIa, IId, and subgroups IIb and IIc. On 6th day, a statistically significant color variation between all the subgroups was analyzed. Maximum variations was seen in subgroups IIa, IIb (–3.91) followed by subgroups IIa and IIc (–3.02). Table 6 shows the comparison between subgroups of Group III when immersed in various solutions on first, third and sixth day. In the present study on 1st day, the maximum variation in The Journal of Indian Orthodontic Society, October-December 2013;47(4):382-389 385 Puneet Yadav et al Table 3: Comparison of base values of Group III with its subgroups IIIa, IIIb, IIIc, IIId on first, third and sixth days respectively Group III No. of sample Base value Mean E Std. deviation Std. error mean T p-value 0.81 0.44 0.13 Group IIIa 1st day 3rd day 6th day 10 10 10 0.98 2.63 3.05 0.29 0.46 0.18 0.09 0.14 0.05 0.99 9.01 14.79 0.333 0.000 0.000 Group IIIb 1st day 3rd day 6th day 10 10 10 5.26 8.20 8.63 0.69 0.56 0.44 0.21 0.17 0.14 17.14 32.55 39.39 0.000 0.000 0.000 Group IIIc 1st day 3rd day 6th day 10 10 10 4.71 5.54 6.11 0.50 0.25 0.28 0.15 0.08 0.09 18.41 29.24 31.80 0.000 0.000 0.000 Group IIId 1st day 3rd day 6th day 10 10 10 3.01 4.50 4.76 0.42 0.32 0.45 0.13 0.10 0.14 11.38 21.36 19.76 0.000 0.000 0.000 p < 0.05, significant Table 4: Intragroup comparison of Group I when immersed in various solutions at days 1, 3 and 6 Groups Ia Ib Ic Mean difference Ib Ic Id Ic Id Id p-value Day 1 Day 3 Day 6 Day 1 Day 3 Day 6 –2.54 –0.92 –1.32 1.62 1.21 –0.40 –4.03 –3.61 –2.57 0.41 1.45 1.03 –3.68 –3.68 –2.01 –0.006 1.66 1.67 0.000 0.187 0.026 0.005 0.047 0.801 0.000 0.000 0.000 0.747 0.007 0.079 0.000 0.000 0.000 1.00 0.000 0.000 p < 0.05, significant Table 5: Intragroup comparison of Group II when immersed in various solutions at days 1, 3 and 6 Groups IIa IIb IIc Mean difference IIb IIc IId IIc IId IId p-value Day 1 Day 3 Day 6 Day 1 Day 3 Day 6 –1.61 –2.16 –0.99 –0.54 0.62 1.16 –1.85 –1.87 –0.23 –0.02 1.61 1.63 –3.91 –3.02 –1.08 0.89 2.83 1.94 0.001 0.000 0.050 0.458 0.346 0.016 0.000 0.000 0.864 1.000 0.000 0.000 0.000 0.000 0.000 0.003 0.000 0.000 p < 0.05, significant Table 6: Intragroup comparison of Group III when immersed in various solutions at days 1, 3 and 6 Groups IIIa IIIb IIIc Mean difference IIIb IIIc IIId IIIc IIId IIId p-value Day 1 Day 3 Day 6 Day 1 Day 3 Day 6 –4.27 –3.72 –2.03 0.54 2.24 1.69 –5.56 –2.90 –1.86 2.66 3.70 1.03 –5.57 –3.05 –1.70 2.52 3.87 1.35 0.000 0.000 0.000 0.085 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 p < 0.05, significant color stability was seen in subgroups IIIa, IIIb again suggesting that tea caused the most significant change in color having value of –4.27 followed by subgroups IIIa and IIIc having value of –3.72. On 3rd day, similar results were found with 386 maximum variation in subgroups IIIa, IIIb with value of –4.27 followed by subgroups IIIb and IIId with value of 2.24. The least significant variation was observed between the Groups IIIc and IIId having a value of 1.69 followed by subgroups IIIa JIOS Evaluation and Comparison of the Color Stability of Various Esthetic Brackets, When Exposed to Various Food Dyes and IIId having value of –2.03. On 6th day, maximum significant variation was seen in IIIa and IIIb having value of –5.57 followed by subgroups IIIa and IIIc with value of –3.05. Statistical analysis was carried out for the comparison of the various esthetic brackets to find out the significant difference between color stability within the Groups. Oneway ANOVA, Turkey multiple comparison test and Students t-test were used for the analysis of results. DISCUSSION Color stability is an important parameter for modern esthetic brackets. Color measurements can be evaluated with various instruments. Since, instrument measurements eliminate the subjective interpretation of visual color comparison, spectrophotometers and colorimeters have been used to measure color change in dental materials. The CIE L*a*b* was chosen to record color differences because it is well suited for determination of small color differences. Commonly E values differing by 1 unit are considered a color match, because they cannot be identified by independent observers.11 Values of E of 2 to 3 represent color difference that are slightly perceptible. E values greater than or equal to 3.3 are visually perceptible and clinically unacceptable.15 Hence, in this study E value below 3.3 were considered clinically acceptable color change. The literature on color stability of orthodontic materials is limited.16,17 In restorative and prosthetic dentistry, various techniques have been described to study discoloration of dental products. Accelerated aging methods and immersion solutions such as coffee, tea, grape juice and chlorhexidine are used for in vitro simulations.18,19 It has been shown that the type of immersion solution and the exposure time influence the degree of color change. Tea and coffee seem to cause the most significant staining.20 In the present study Group I ceramic brackets showed maximum stains with coffee with E value of 5.18 on 6th day than tea with E value of 5.17, whereas Group II brackets revealed maximum stains with tea carrying E value of 5.05 than coffee with E value of 4.16. The composite brackets showed maximum stains with both tea and coffee with “E value of 8.63 and 6.11 consecutively on 6th day. This could be explained due to hydrophilic nature of resin matrix in composite brackets. It is known that composite material allows water to penetrate the matrix and the filler matrix interface.21,22 The interface between resin and particles is a weak point of a composite material with high sensitivity to water sorption.23 Therefore, the hydrophilic degradation of this interface might lead to enhanced water uptake of highly filled brackets. Water acts as a carrier, and, as a result, solvent food dyes could penetrate the composite and discolor the bracket. Hence, the composite brackets attained more stains than ceramic brackets. The color stability of composites can be affected due to many variables.24 Ferracane et al25 investigated the cause of yellow discoloration and found that yellowing of the polymer was accompanied by a reduction in the quantity of residual unreacted double bonds in the resins. They stated that possible explanation for the yellowing could be an oxidation of the unreacted C = C to produce colored peroxide compounds. Thus, the polymeric structure and filler content as well as the polymerization conversion, seem to be the most important factors, which influence the color stability of dental polymers. Sham et al13 believed that changes in the optical properties in the polymer could be responsible for color change. The discoloration could be either due to extrinsic or intrinsic factors. Extrinsic factors include adsorption or absorption of colorants as a result of contamination from exogenous sources. Extrinsic factors for discoloration are known to cause staining of oral tissues and restorations especially in combination with dietary factors. Among these tea, coffee, nicotine and beverages have been reported. In the present study, discoloration of resin-based composite brackets by tea was mainly due to surface adsorption of the colorants and discoloration by coffee was due to adsorption and also due to absorption of colorants by investigated materials. Absorption and penetration of colorants into the organic phase of the resin-based materials are probably due to compatibility of the polymer phase with the yellow colorants of coffee. There was a small increase in the discoloration of the brackets on the 1st day, and it increased with time that is on 6th day, and this correlated with the present study findings.26,27 Also Groups II and III brackets showed maximum discoloration with tea rather than coffee on 6th day having E value of 5.05 and 8.63 consecutively. This is in contrast to studies done by authors who used tea, coffee as chromogenic agents for resin restorative material and concluded coffee to stain more than tea.20,28 In a study done by Ruyter et al29 staining of resin based veneering materials with three heat cured and two light cured resins on test materials showed more discoloration by tea than coffee over an observation period of 48 hours. But at the same time, it reported that staining with tea was superficial and more easily removed in comparison to coffee stains after cleansing treatment with soap and toothbrush. In the present study, the discoloration with composites brackets was more than Group II and I brackets having E value of 8.63 and 6.11 with tea and coffee respectively on 6th day. Gharamanlooet al30 stated that porcelains resist discoloration whereas composites are susceptible to extrinsic and intrinsic stains. They acknowledged that high glossy surface is less susceptible to staining, other surface conditions as incomplete polymerization of the resin matrix may lead to surface staining of composites. Microcracks, microvoids or interfacial gaps located at the interface between filler and matrix are the penetration pathways for stains. When Groups I, II and III were compared with subgroups tea, coffee, coke and drinking water on 1st, 3rd and 6th day, tea and coffee stained more than coke and drinking water respectively. With coffee staining more than tea, are similar to findings by Lu et al31 and Guler et al.5 On 3rd day, the maximum The Journal of Indian Orthodontic Society, October-December 2013;47(4):382-389 387 Puneet Yadav et al changes in the color stability was observed between the Groups Ia, Ib having values –4.03 and Ia, Ic (–3.61) which was similar to results shown by Faltermiere et al.1 Coffee stained more than other subgroups which was in accordance with findings by Gupta et al.32 Koksal and Dikbas2 suggested that coffee stained the brackets the most. The least significant variation was seen between subgroups IIb and IIc having value of 0.89. Faltermeier et al33 investigated the influence of electron beam irradiation with an energy dose of 100 kGy on the mechanical properties and color stability of conventional polymer brackets and experimental filled composite brackets. The influence of electron beam postcuring on Vickers hardness (VH) of the polymer brackets was investigated and possible discoloration of the brackets after electron beam irradiation was determined according to the three-dimensional L* a* b* color space. These results demonstrated that the mechanical properties of polymer brackets could be modified by electron beam irradiation but clinical use of electron beam postcuring might be restricted because of unacceptable color changes. The color stability of ceramic and composite brackets was found to depend on many factors, such as filler level and type of discoloration. According to Lee YK the kind of material and crystal structure for ceramic brackets did not influence color stability, but color stability was mainly branddependent.34 Despite our results, the clinical performance of brackets depends on various synergistic effects in the oral environment that cannot be simulated precisely by in vitro investigations. When discussing the clinical application of these results, it must be considered that the oral environment differs in several ways from in vitro conditions. Factors such as variety of foods, thermal and mechanical stresses and their interactions may intensify the discolorations in vivo. Since a limited data is available in orthodontics, further in vivo studies should be carried out for a longer period of time in this field to evaluate discoloration as well as clinical color stability. CONCLUSION The brackets of Group I showed maximum variation in the color stability when immersed in coffee solution, which was followed by tea and coke respectively. However, this variation was clinically insignificant whereas the brackets of Group II showed maximum variation in the color stability when immersed in tea solution. Group III showed maximum variation in the color stability when immersed in tea solution, which was followed by coffee, coke solutions and drinking water respectively. Composite brackets showed overall poor color stability with all the solutions. According to Ruyter et al14 a 'E* of 3.3 is visually perceptible and therefore clinically unacceptable. In this in vitro investigation, maximum exposure time of 6 days was chosen. In spite of this short exposure period, almost all 388 investigated esthetic brackets showed undesirable discoloration. 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