Download Preparation of reagents

Preparation of reagents
Chlorine solution + 3 other reagents
Hand rub
Pipette control (calibration) + actions
1 Chlorine preparation
1 Chlorine preparation
1 Chlorine preparation
active product = non-dissociated HOCl
(hypchloric acid)
1.
2.
3.
4.
Liquid (Household bleach,…)
Powder (Calcium Hypochloric acid)
Chlorine-releasing tabs
Granules (NaDCC tabs)
1 Chlorine preparation
Chlorine, active compound
active product = non-dissociated HOCl
Amounts of active chlorine = ppm
(parts per million)
On the package: expressed as “%”
(weight or volume)
On the package: expressed as “°”
(French-speaking countries, degrées
chlorimétriques)
Chlorine dilution efficacy level
ppm
%
Dilution of
5% Bleach
Activity
100
Bacteria
500
+ Bacillus spores
2500
+ Viruses
5000
10.000
50.000
+ M.tuberculosis
remember decontamination
remember clean-up of spills
1 Chlorine preparation
Find the products (company).
Base products : liquid, solid (tablets), solid
(powder)
Selection of the better product.
MSDS (Material Safety Data Sheets) and
where to find them.
Safety precautions to be used.
Preparation of the product itself.
Flask, label, storage, …
+ verify the concentration of available free chlorine
+ 3 other reagents
Verify the concentration of
available free chlorine
1 Chlorine preparation
Find the products (company).
MSDS (Material Safety Data Sheets) and where to
find them.
Safety precautions to be used.
Preparation of the product itself.
Flask, label, storage, …
Base products : liquid, solid (tablets), solid
(powder)
+ verify the concentration of available free chlorine
+ 3 other reagents
+ 3 other reagents
+ 3 other reagents
Make 50 ml of tri-Sodium Citrate 3,2 % w/v.
Prepare 500 ml of di-Sodium Hydrogen
Phosphate (stock solution B for the
preparation of buffer water): di-Sodium
Hydrogen Phosphate dihydrate 11,87 g/l.
Calculate the Molarity of this solution.
Make 500 ml of Formol Saline 10 % (v /v)
solution. NB : (Physiological) Saline Solution
= NaCl 0,85 % (w/v).
Preparation of reagents
Preparation of reagents
2. Hand rub
2. Hand rub
2. Hand rub
2 Hand rub product :
Alcohol based formulation for in house/local production (WHO) :
Document: Guidelines on Hand Hygiene in Health Care (Advanced Draft)
WHO Headquarters. Geneva, Switzerland, in pdf format
http://whqlibdoc.who.int/hq/2005/WHO_EIP_SPO_QPS_05.2.pdf
2. Hand rub
2. Hand rub
2. Hand rub preparation
Find the products (company).
MSDS (Material Safety Data Sheets)
and where to find them.
Safety precautions to be used.
Preparation of the product itself.
Flask, label, storage, …
2. Hand rub preparation