Download Certificate of Conformance for aTRAQ Internal Standard

Certificate of Analysis
Product Identification
Name: aTRAQ™ INTERNAL STANDARD
Part number: 4442688
Lot Specific Information
Lot number:A1283
Date Manufactured: 10/21/2011
Reconstitution:
Diluent
Target Volume Range
Reconstitution Volume
4374866
1.76 – 2.00 mL
1.78 mL
Internal Standard Amino Acid Values
Amino Acid
O‐Phospho‐L‐serine
O‐Phosphoethanolamine
Taurine
L‐Asparagine
L‐Serine
Glycine
Hydroxy‐L‐proline
Ethanolamine
L‐Glutamine
L‐Aspartic Acid
L‐Methionine sulfoxide
L‐Citrulline
L‐Threonine
L‐Sarcosine
β‐Alanine
L‐Alanine
L‐Glutamic Acid
L‐Histidine
1‐Methyl‐L‐histidine
3‐Methyl‐L‐histidine
Argininosuccinic Acid
L‐Homocitrulline
L‐Anserine
L‐Carnosine
L‐α‐Aminoadipic Acid
γ‐Amino‐n‐butyric Acid
D,L‐ ‐Aminoisobutyric Acid
L‐α‐Amino‐n‐butyric Acid
L‐Arginine
L‐Proline
L‐Ornithine
Cystathionine
L‐Cystine
δ‐D,L‐Hydroxylysine
L‐Lysine
L‐Methionine
L‐Valine
L‐Norvaline
L‐Tyrosine
L‐Homocystine
L‐Isoleucine
L‐Leucine
L‐Norleucine
L‐Phenylalanine
L‐Tryptophan
AB Sciex Pte. Ltd.
110 Marsh Road
Foster City, CA 94404
U.S.A.
Amino
Acid Code
PSer
PEtN
Tau
Asn
Ser
Gly
Hyp
EtN
Gln
Asp
MOx
Cit
Thr
Sar
bAla
Ala
Glu
His
1MHis
3MHis
Asa
Hcit
Ans
Car
Aad
GABA
bAib
Abu
Arg
Pro
Orn
Cth
Cys
Hyl
Lys
Met
Val
Nva
Tyr
Hcy
Ile
Leu
Nle
Phe
Trp
Reconstituted
Concentration (μM)
5.23
4.77
5.06
4.77
4.91
4.20
5.25
4.44
4.21
5.44
6.51
4.95
4.51
5.06
4.43
4.87
5.19
4.93
4.65
4.92
2.16
4.24
4.91
4.76
4.83
5.88
5.22
4.98
5.04
4.88
4.64
3.42
2.46
4.67
5.11
1.71
5.05
4.62
4.98
5.11
5.11
5.03
4.82
4.64
5.17
Hydolysate Calculation
Amount (pmole)a
ND
ND
ND
ND
9.83
8.39
ND
ND
ND
10.88
13.02
ND
9.03
ND
ND
9.75
10.39
9.87
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
10.09
9.76
ND
ND
4.91
ND
10.22
3.41
10.10
9.23
9.97
ND
10.22
10.06
9.64
9.29
ND
Physiological Calculation
Concentration (μM)b
104.5
95.4
101.3
95.3
98.3
83.9
105.0
88.8
84.3
108.8
ND
99.0
90.3
101.3
88.6
97.5
103.9
98.7
93.0
98.4
43.2
84.9
98.1
95.3
96.5
117.6
104.3
99.6
100.9
97.6
92.9
68.4
49.1
93.4
102.2
34.1
101.0
92.3
99.7
102.2
102.2
100.6
96.4
92.9
103.3
Document No.: 4444907
Revision: B
Certificate of Analysis
a
Internal Standard (IS) amount to use for calculating the analyte amount on column from the Hydrolysate
Sample Assay using the following formula:
Analyte Amount (pmole) = Analyte Area x IS Amount (pmole) / IS Area
b
Internal Standard (IS) concentration to use for calculating the analyte concentrations in the original
physiological sample from the Physiological Sample Assay using the following formula:
Analyte Concentration (µM) = Analyte Area x IS Concentration (µM) / IS Area
ND‐ Not determined by the quantitation method.
The reconstituted aTRAQ Internal Standard also contains approximately 5 μM of labeled ammonium
chloride and unlabeled urea and creatinine.
Quality Assurance Statement:
The information on this certificate as been reviewed and accurately reflects the manufacturing and
analysis data for the product lot specified above.
Quality Assurance Associate:Josh Whitesell
Date:10/21/2011
Support
Technical Support call 1 800-952-4716 or 877-740-2129 or E-mail: [email protected]
This certificate may be obtained at: http://www.sciex.com/downloads/certificates-of-analysis
AB Sciex Pte. Ltd.
110 Marsh Road
Foster City, CA 94404
U.S.A.
Document No.: 4444907
Revision: B