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Certificate of Analysis Product Identification Name: aTRAQ™ INTERNAL STANDARD Part number: 4442688 Lot Specific Information Lot number:A1283 Date Manufactured: 10/21/2011 Reconstitution: Diluent Target Volume Range Reconstitution Volume 4374866 1.76 – 2.00 mL 1.78 mL Internal Standard Amino Acid Values Amino Acid O‐Phospho‐L‐serine O‐Phosphoethanolamine Taurine L‐Asparagine L‐Serine Glycine Hydroxy‐L‐proline Ethanolamine L‐Glutamine L‐Aspartic Acid L‐Methionine sulfoxide L‐Citrulline L‐Threonine L‐Sarcosine β‐Alanine L‐Alanine L‐Glutamic Acid L‐Histidine 1‐Methyl‐L‐histidine 3‐Methyl‐L‐histidine Argininosuccinic Acid L‐Homocitrulline L‐Anserine L‐Carnosine L‐α‐Aminoadipic Acid γ‐Amino‐n‐butyric Acid D,L‐ ‐Aminoisobutyric Acid L‐α‐Amino‐n‐butyric Acid L‐Arginine L‐Proline L‐Ornithine Cystathionine L‐Cystine δ‐D,L‐Hydroxylysine L‐Lysine L‐Methionine L‐Valine L‐Norvaline L‐Tyrosine L‐Homocystine L‐Isoleucine L‐Leucine L‐Norleucine L‐Phenylalanine L‐Tryptophan AB Sciex Pte. Ltd. 110 Marsh Road Foster City, CA 94404 U.S.A. Amino Acid Code PSer PEtN Tau Asn Ser Gly Hyp EtN Gln Asp MOx Cit Thr Sar bAla Ala Glu His 1MHis 3MHis Asa Hcit Ans Car Aad GABA bAib Abu Arg Pro Orn Cth Cys Hyl Lys Met Val Nva Tyr Hcy Ile Leu Nle Phe Trp Reconstituted Concentration (μM) 5.23 4.77 5.06 4.77 4.91 4.20 5.25 4.44 4.21 5.44 6.51 4.95 4.51 5.06 4.43 4.87 5.19 4.93 4.65 4.92 2.16 4.24 4.91 4.76 4.83 5.88 5.22 4.98 5.04 4.88 4.64 3.42 2.46 4.67 5.11 1.71 5.05 4.62 4.98 5.11 5.11 5.03 4.82 4.64 5.17 Hydolysate Calculation Amount (pmole)a ND ND ND ND 9.83 8.39 ND ND ND 10.88 13.02 ND 9.03 ND ND 9.75 10.39 9.87 ND ND ND ND ND ND ND ND ND ND 10.09 9.76 ND ND 4.91 ND 10.22 3.41 10.10 9.23 9.97 ND 10.22 10.06 9.64 9.29 ND Physiological Calculation Concentration (μM)b 104.5 95.4 101.3 95.3 98.3 83.9 105.0 88.8 84.3 108.8 ND 99.0 90.3 101.3 88.6 97.5 103.9 98.7 93.0 98.4 43.2 84.9 98.1 95.3 96.5 117.6 104.3 99.6 100.9 97.6 92.9 68.4 49.1 93.4 102.2 34.1 101.0 92.3 99.7 102.2 102.2 100.6 96.4 92.9 103.3 Document No.: 4444907 Revision: B Certificate of Analysis a Internal Standard (IS) amount to use for calculating the analyte amount on column from the Hydrolysate Sample Assay using the following formula: Analyte Amount (pmole) = Analyte Area x IS Amount (pmole) / IS Area b Internal Standard (IS) concentration to use for calculating the analyte concentrations in the original physiological sample from the Physiological Sample Assay using the following formula: Analyte Concentration (µM) = Analyte Area x IS Concentration (µM) / IS Area ND‐ Not determined by the quantitation method. The reconstituted aTRAQ Internal Standard also contains approximately 5 μM of labeled ammonium chloride and unlabeled urea and creatinine. Quality Assurance Statement: The information on this certificate as been reviewed and accurately reflects the manufacturing and analysis data for the product lot specified above. Quality Assurance Associate:Josh Whitesell Date:10/21/2011 Support Technical Support call 1 800-952-4716 or 877-740-2129 or E-mail: [email protected] This certificate may be obtained at: http://www.sciex.com/downloads/certificates-of-analysis AB Sciex Pte. Ltd. 110 Marsh Road Foster City, CA 94404 U.S.A. Document No.: 4444907 Revision: B