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Murine Macrophage Mannose Receptor Promoter Is Regulated by the Transcription Factors PU.1 and SP1 by Quentin Eichbaum, David Heney, David Raveh, Michael Chung, Mark Davidson, Judith Epstein, and R. Alan B. Ezekowitz Blood Volume 90(10):4135-4143 November 15, 1997 ©1997 by American Society of Hematology Murine MR 5′ flanking region. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology Transfection of wild-type MR promoter constructs into myeloid and nonmyeloid cells. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology Identification of PU.1 binding to the MR promoter by gel mobility shift analysis. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology Mutational analysis of the −164 and −177 PU.1 sites. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology Decreased transcriptional activity of promoter activity of promoter fragments containing mutated PU.1 and/or Sp1 sites. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology Sp1 and PU.1 act synergistically in cotransactivating transcription of the murine MR promoter. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology Identification of Sp1 binding to the MR promoter by electrophoretic mobility shift analysis. Quentin Eichbaum et al. Blood 1997;90:4135-4143 ©1997 by American Society of Hematology