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Photosynthesis and Rate Aims: Must be able to outline the factors that affect the rate of photosynthesis in plants. Should be able to explain why these factors affect photosynthetic rate. Could be able to outline the energy transfers involved in environments. Factors Affecting Photosynthetic Rate The rate at which plants can make food (the photosynthetic rate) is dependent on environmental factors. These include: the amount of light available. the level of carbon dioxide (CO2). the temperature. Factors Affecting Photosynthetic Rate The effect of light intensity on photosynthetic rate is shown. Experiment was carried out at a constant temperature and constant carbon dioxide level. The rate of photosynthesis increases exponentially with light intensity until a maximum rate is achieved. At this point increasing the light intensity has no effect on photosynthetic rate and the rate of photosynthesis reaches a plateau. Rate of photosynthesis (mm3CO2 cm-2h-1) Light intensity vs photosynthetic rate 90 80 70 60 50 40 1 2 3 4 5 6 Units of light intensity (arbitrary scale) 7 Photosynthetic rate increases as the CO2 concentration increases. At high concentrations, the rate of photosynthesis begins to slow as limiting factors other than CO2 become important. An increase in temperature increases photosynthetic rate because of its effect on enzyme activity. Temperatures exceeding the optimum will decrease due to denaturation of enzyme Light intensity, CO2, and temperature vs photosynthetic rate Rate of photosynthesis (mm3CO2 cm-2h-1) Factors Affecting Photosynthetic Rate High CO2 at 30°C 240 200 High CO2 at 20°C 160 120 Low CO2 at 30°C 80 40 Low CO2 at 20°C 1 2 3 4 5 6 Units of light intensity (arbitrary scale) Increasing the temperature when CO2 is limiting has little effect on photosynthetic rate. 7 Action Spectrum An action spectrum profiles the effectiveness of different wavelength light in fueling photosynthesis. It is obtained by plotting wavelength against some measure of photosynthetic rate (e.g. CO2 production). Rate of photosynthesis (as percent of rate at 670 nm) 100 Action spectrum for photosynthesis (Effectiveness of different wavelengths in fueling photosynthesis) 80 60 40 20 The action spectrum closely matches the absorption spectrum for the photosynthetic pigments. 0 400 500 Wavelength (nm) 600 700 Limiting Factors Light Intensity, Carbon dioxide concentration, Temperature or chlorophyll concentration (affected by the amount of Nitrogen present) are the main LIMITING FACTORS of photosynthesis. The above factor that is furthest from it’s optimum level for photosynthesis will control the amount of photosynthesis that takes place. E.g. if the temperature of the plant is furthest from it’s optimal level then the increasing the amount of carbon dioxide and light will not affect the rate of photosynthesis. Questions Biozone Book: Answer questions from p49 Complete p44 - 48 Summary of Energy Transformations Sunlight is primary source of energy in Autotrophs and Heterotrophs: Sunlight energy – chemical energy in Glucose (PHOTOSYNTHESIS). Chemical energy released to produce ATP (RESPIRATION) [more easily transported form]. Can be stored as GLYCOGEN in animals or as FAT in adipose cells when liver is full, STARCH in plants (mostly roots). ATP releases energy as required for growth, repair, communication, movement, Active Transport, reproduction and biosynthesis.