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Transcript 
Truncated Estrogen Receptor α 46-kDa Isoform in Human
Endothelial Cells
by Gemma A. Figtree, Denise McDonald, Hugh Watkins, and Keith M. Channon
Circulation
Volume 107(1):120-126
January 7, 2003
Copyright © American Heart Association, Inc. All rights reserved.
Figure 1. A, RT-PCR demonstrating the expression of Δ1a-hERα-46 transcript (lower bands) in
human endothelial-derived cell lines EA.926, hMECs, HUVECs, and the positive control MCF-7
cells.
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
Figure 2. Western blot demonstrating a 46-kDA ERα immunoreactive band in lysates of both
MCF cells and EA.926 cells.
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
Figure 3. Cells transfected with GFP-tagged ERα isoforms.
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
Figure 4. eNOS immunostaining on COS cells cotransfected with eNOS and GFP-tagged ERα
isoforms.
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
Figure 5. Nonpermeabilized COS cells transfected with Δ1a-hERα-46 (ERα46) or ERα66
expression vectors contain surface binding sites for 17β-estradiol (E2) detectable by cellimpermeant ligand binding (E2coBSA covalently linked to E2-FITC).
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
Figure 6. Estrogen-induced NO production in DAF-2DA–loaded, transfected COS cells.
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
Figure 7. Transient transfection studies using the estrogen response element reporter plasmid
(ERE)2-tk-LUC to examine the relative estrogen-dependent transcriptional activation mediated
by Δ1a-hERα-46 (ERα46) compared with ERα66. Δ1a-hERα-46 has much less ability for estrogendependent transcriptional activity and inhibits that of ERα66 in human endothelial cells.
Gemma A. Figtree et al. Circulation. 2003;107:120-126
Copyright © American Heart Association, Inc. All rights reserved.
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