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Activation of the Human Immunodeficiency Virus-1 Long Terminal Repeat by Respiratory Burst Oxidants of Neutrophils by Seymour J. Klebanoff, and Catherine M. Headley Blood Volume 93(1):350-356 January 1, 1999 ©1999 by American Society of Hematology Activation of the HIV-1 LTR in Jurkat T cells by neutrophils and PMA. The reaction mixture contained 2 × 106 Jurkat LTRluc and, where indicated, 100 ng/mL PMA and either normal or CGD neutrophils at the concentrations indicated, in RPMI at a final volume of... Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology PMA dose response curve. Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology Effect of catalase, heated catalase, or SOD on the activation of the LTR by neutrophils and PMA. The reaction mixture contained 2 × 106 Jurkat LTRlucand, where indicated, 100 ng/mL PMA, 106 or 107neutrophils/mL, 8.2 μg/mL catalase, 8.2 μg/mL heated catalase... Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology Effect of vanadate on the activation of the LTR by neutrophils and PMA. The reaction mixture contained 2 × 106 Jurkat LTRluc and, where indicated, 3 × 10−5 mol/L vanadate, 100 ng/mL PMA, and neutrophils at the levels indicated, in RPMI at a final volume of ... Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology Effect of azide on the activation of the LTR by neutrophils and either PMA or opsonized zymosan. Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology Synergistic effect of H2O2 and PMA on activation of the LTR. The reaction mixture contained 2 × 106 Jurkat LTRluc and, where indicated, 100 ng/mL PMA, 100 U/mL TNF- , 100 U/mL IL-2, and H2O2 at the concentrations indicated in RPMI at a final volume of 2.0 mL. Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology Effect of the PKC inhibitors BIM and Gö 6976 on activation of the LTR. The reaction mixture contained 2 × 106 Jurkat LTRluc and where indicated 100 ng/mL PMA, 10−4 mol/L H2O2, 106 neutrophils/mL, 100 U of TNF- /mL, and BIM and Gö 6976 at the concentrations ... Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology Effect of the PKC inhibitor BIM on the respiratory burst of neutrophils induced by PMA and opsonized zymosan. Seymour J. Klebanoff, and Catherine M. Headley Blood 1999;93:350-356 ©1999 by American Society of Hematology