Download Powerpoint - Circulation Research

Localization of Atrial Natriuretic Factor Receptors in the
Mesenteric Arterial Bed
by Héctor de León, Marie-Chantal Bonhomme, Gaétan Thibault, and Raul Garcia
Circulation Research
Volume 77(1):64-72
July 1, 1995
Copyright © American Heart Association, Inc. All rights reserved.
Low-power magnifications (original magnifications ×1.5) of mesenteric arterial bed consisting of
superior mesenteric, jejunal, and ileal arteries.
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
Pharmacological characterization of atrial natriuretic factor (ANF) receptors in mesenteric
adipose and vascular tissues. a and b, Representative 125I-ANF saturation binding curves in
membranes prepared from mesenteric adipose tissue (a) and arteries (b). ○ indicates total
binding; ▿, nonspecific binding; and •, specific binding. c, Scatchard transformation of specific
binding data in mesenteric adipose tissue (Bmax, 404 fmol/mg protein; Kd, 333 pmol/L) and
vascular membranes (○). d, Representative competitive inhibition curves of 125I-ANF binding to
mesenteric adipose tissue. • indicates ANF; □, C-type natriuretic peptide; and ○, des[Gln18,Ser19,Gly20,Leu21,Gly22]ANF-(4-23).
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
Pharmacological characterization of angiotensin II (Ang II) receptors in mesenteric adipose and
vascular tissues. a and b, Representative 125I–[Sar1,Ile8]Ang II saturation binding curves in
membranes prepared from mesenteric fat (a) and arteries (b). ○ indicates total binding; ▿,
nonspecific binding; and •, specific binding. c, Scatchard transformation of the specific binding
data. • indicates fatty membranes (Bmax, 210 fmol/mg protein; Kd, 500 pmol/L); ○, vascular
membranes (Bmax, 15 fmol/mg protein; Kd, 580 pmol/L). d, Representative competitive inhibition
curves of 125I–[Sar1,Ile8]Ang II binding to mesenteric adipose tissue. • indicates [Sar1,Ile8]Ang
II; ○, Ang II; ▿, losartan; and □, PD123319.
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
Pharmacological characterization of endothelin-1 (ET-1) receptors in mesenteric adipose and
vascular tissues. a, Representative 125I–ET-1 saturation specific-binding curves in membranes
prepared from mesenteric adipose tissue (•) and arteries (○). b, Scatchard transformation of
specific binding data in mesenteric adipose tissue (•; Bmax, 390 fmol/mg protein; Kd, 600
pmol/L) and mesenteric arteries (○; Bmax, 205 fmol/mg protein; Kd, 200 pmol/L). c and d,
Representative competitive inhibition curves of 125I–ET-1 binding to mesenteric arteries (c) and
mesenteric adipose tissue (d). ○ indicates ET-1; •, BQ-123.
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
Graphs showing cGMP stimulation by atrial natriuretic factor (ANF), C-type natriuretic peptide
(CNP), and des-[Gln18,Ser19,Gly20,Leu21,Gly22]ANF-(4-23) (C-ANF) in mesenteric adipose
tissue (a) and by ANF, CNP, C-ANF, and sodium nitroprusside (SNP) in mesenteric arteries (b).
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
Autoradiography by SDS-PAGE of cross-linked atrial natriuretic factor (ANF) receptors in
adipose (lanes 1 through 4) and vascular (lanes 5 and 6) tissues in the absence (lanes 1 and 5)
and presence (lanes 2 and 6) of 10−6 mol/L ANF, des-[Gln18,Ser19,Gly20,Leu21,Gly22]ANF-(4-23)
(lane 3), and C-type natriuretic peptide (lane 4).
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
In situ autoradiographic localization of 125I–atrial natriuretic factor binding sites in the
mesentery in the absence (a) and presence (b) of unlabeled atrial natriuretic factor (original
magnification ×160).
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
In vitro autoradiographic localization of 125I–endothelin-1 binding sites in mesenteric arterioles
(a and b) and surrounding adipose tissue (c and d) in the absence (a and c) and presence (b and
d) of unlabeled endothelin-1 (original magnification ×400).
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.
Tracings showing representative responses of isolated defatted, perfused mesenteric arteries to
several agents.
Héctor de León et al. Circ Res. 1995;77:64-72
Copyright © American Heart Association, Inc. All rights reserved.