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Lesson Overview Energy and Life Lesson Overview 8.1 Energy and Life Lesson Overview Energy and Life Chemical Energy and ATP o Adenosine Triphosphate (ATP) – VERY IMPORTANT ENERGY MOLECULE o ATP consists of 1. adenine 2. 5-carbon sugar called ribose 3. three phosphate groups Lesson Overview Energy and Life Using Biochemical Energy o ATP is not good for storing energy or for long term use o cells keep small amount of ATP for use o Cells can regenerate ATP by using the energy in foods like glucose Lesson Overview Energy and Life Heterotrophs and Autotrophs o Heterotrophs - obtain food by consuming other living things; various types (herbivores, carnivores, etc) o Autotrophs - organisms that make their own food; generally use sunlight to make food (photosynthesis) Lesson Overview Energy and Life Lesson Overview 8.2 Photosynthesis: An Overview http://www.wwnorton.com/college/biology/discoverbio3/core/ content/index/animations.asp Lesson Overview Energy and Life Light Sunlight is a mixture of different wavelengths, many of which are visible to our eyes and make up the visible spectrum Lesson Overview Energy and Life Pigments Plants gather the sun’s energy with light-absorbing molecules called pigments chlorophyll – main pigment in plants Lesson Overview Energy and Life Pigments Two types of chlorophyll found in plants: chlorophyll a and chlorophyll b Another pigment found in plants carotene Lesson Overview Energy and Life Chloroplasts Photosynthesis takes place inside chloroplasts Chloroplast structure includes: • • • • double membrane thylakoids (contain pigments) grana (stacks of thylakoids) stroma (fluid) Lesson Overview Energy and Life Energy Collection When chlorophyll absorbs light, the energy is transferred to electrons Lesson Overview Energy and Life High-Energy Electrons • Energy in electrons must be transferred to other molecules to make food for the plant • Electron carriers are used to transfer the energy to another molecule Lesson Overview Energy and Life High-Energy Electrons NADP+ - a carrier molecule used in photosynthesis NADPH - carry the high-energy electrons to chemical reactions elsewhere in the cell Lesson Overview Energy and Life An Overview of Photosynthesis Photosynthesis uses the energy of sunlight to convert water and carbon dioxide into high-energy sugars and oxygen. Lesson Overview Energy and Life PHOTOSYNTHESIS Photosynthesis involves two sets of reactions: 1. light-dependent reactions 2. light-independent reactions Lesson Overview Energy and Life Light-Dependent Reactions The light-dependent reactions use sunlight & water to produce ATP , NADPH (energy molecules), and oxygen Take place within the thylakoid membranes Lesson Overview Energy and Life Light-Independent Reactions During light-independent reactions, ATP, NADPH, and carbon dioxide are used to produce high-energy sugars Light-independent reactions take place in the stroma (fluid) Lesson Overview Energy and Life Lesson Overview 8.3 The Process of Photosynthesis Lesson Overview Energy and Life The Light-Dependent Reactions: Generating ATP and NADPH Photosystems (clusters of chlorophyll/proteins) absorb sunlight and generate high-energy electrons that are then passed to a series of electron carriers embedded in the thylakoid membrane Lesson Overview Energy and Life Photosystem II Light energy is absorbed in the pigments within photosystem II, “exciting” electrons These electrons are passed to the electron transport chain (ETC) Lesson Overview Energy and Life Photosystem II Enzymes of the inner surface of the thylakoid break up water molecules into 2 electrons, 2 H+ ions, and 1 oxygen atom: 2 electrons – go to PS II to replace other electrons Oxygen – released into atmosphere H+ - create gradient to create ATP ***WATER IS A PRETTY IMPORTANT PART OF THE EQUATION!!! Lesson Overview Energy and Life Electron Transport Chain Energy from the electrons used to pump H+ from stroma into thylakoid space Electrons wind up at PS I (chlorophyll/proteins); need to be “recharged/re-energized” by sunlight Lesson Overview Energy and Life Photosystem I At end of ETC, electrons combine with H+ and NADP+ to make NADPH Lesson Overview Energy and Life Hydrogen Ion Movement and ATP Formation H+ ions accumulate within the thylakoid space during process, creating a gradient This gradient provides the energy to make ATP (using ATP synthase to pump H+) - chemiosmosis Lesson Overview Energy and Life Summary of Light-Dependent Reactions The light-dependent reactions produce oxygen, ATP, and NADPH ATP and NADPH will be used in LIGHT-INDEPENDENT REACTIONS to make sugar Lesson Overview Energy and Life The Light-Independent Reactions: Producing Sugars Light-independent reactions, also known as Calvin cycle, require CO2, ATP, and NADPH to make sugar Takes place in stroma Lesson Overview Energy and Life Carbon Dioxide Enters the Cycle CO2 molecules enter the Calvin cycle from the atmosphere An enzyme (rubisco) in the stroma combines carbon dioxide molecules with 5-carbon compounds = CARBON FIXATION Lesson Overview Energy and Life Carbon Dioxide Enters the Cycle The 3-carbon compounds use energy from ATP and NADPH to change throughout the cycle Lesson Overview Energy and Life Sugar Production 2 3-carbon molecules leave the cycle to become food for the plant The rest of the carbon compounds are converted back into the beginning 5 carbon compounds to start cycle again Lesson Overview Energy and Life Factors Affecting Photosynthesis Among the most important factors that affect photosynthesis are temperature, light intensity, and the availability of water. • Average temp best (due to enzyme activity) • high light intensity (up to a point) • lack of water will slow or prevent photosynthesis Lesson Overview Energy and Life Photosynthesis Under Extreme Conditions Most plants under bright, hot conditions close the small openings in their leaves that normally admit carbon dioxide (to conserve water) Carbon dioxide falls to very low levels, slowing down or even stopping photosynthesis. C4 and CAM plants have biochemical adaptations that minimize water loss while still allowing photosynthesis to take place in intense sunlight C4 plants – corn, sugarcane CAM plants – pineapple, cacti