Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Powerpoint - Blood Journal
Transcript
Systemic circulation of poly(l-lysine)/DNA vectors is influenced by polycation molecular weight and type of DNA: differential circulation in mice and rats and the implications for human gene therapy by Christopher M. Ward, Martin L. Read, and Leonard W. Seymour Blood Volume 97(8):2221-2229 April 15, 2001 ©2001 by American Society of Hematology pLL211/DNA complexes exhibit increased circulation in mice compared to pLL20/DNA complexes and are unaffected by the type of DNA used.pLL/DNA complexes, formed with pLL20 (20 kd pLL/DNA) or pLL211 (211 kd pLL/DNA), and either DNAct(▪), DNAcp (■), or DNAlp (... Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNA complexes display increased aggregation in salt compared to pLL211/DNA.pLL/DNAcp complexes were analyzed for light scattering on a Zetasizer photon correlation spectroscope in the presence or absence of 150 mM NaCl. pLL20/DNAcp (●, +NaCl; ♦, −NaCl... Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNA complexes, but not pLL211/DNA, show punctate distribution of radioactivity in blood smears, and both types of complexes bind erythrocytes.pLL20/DNAct or pLL211/DNAct complexes were administered intravenously to female Balb/c mice (0.1 mL of 10 μg/... Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNA and pLL211/DNA complexes associate with Kupffer cells in mice.pLL20/DNAct or pLL211/DNAct complexes were administered intravenously to female Balb/c mice (0.1 mL of 10 μg/mL DNA). Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNAct complexes, but not pLL211/DNA, fix mouse complement C3 independently of immunoglobulins.Biotin-pLL20/DNAct or biotin-pLL211/DNAct complexes were immobilized on streptavidin magnetic beads and incubated in mouse serum at 37°C for 5 minutes. Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNA complexes lead to increased levels of CR3-expressing cells within the liver of mice.Method as for Figure 4. Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNAct and pLL211/DNAct complexes are rapidly removed from the circulation of female Wistar rats but do not bind complement C3 in vitro.(A) pLL20/DNAct (♦) or pLL211/DNAct (▪) complexes were administered intravenously to female Wistar rats (1.0 mL of 1... Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology pLL20/DNAct and pLL211/DNAct complexes fix human complement C3 and bind IgG but are not hematotoxic in vitro.(A) Hematotoxicity of pLL20/DNAct (■) or pLL211/DNAct (▪) complexes was determined by their incubation in human or rat blood at 37°C for 1 hour. Christopher M. Ward et al. Blood 2001;97:2221-2229 ©2001 by American Society of Hematology
