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MIP-1α and MIP-1β differentially mediate mucosal and systemic adaptive immunity by James W. Lillard, Udai P. Singh, Prosper N. Boyaka, Shailesh Singh, Dennis D. Taub, and Jerry R. McGhee Blood Volume 101(3):807-814 February 1, 2003 ©2003 by American Society of Hematology OVA-specific serum IgA, IgM, IgG, and IgE Ab responses following nasal immunization with MIP1α or MIP-1β.Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■) or 1.0 μg MIP-1α or MIP-1β (▪) in PBS. The data present... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology OVA-specific serum IgG subclass Ab responses following nasal immunization with MIP-1α or MIP-1β.Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■) or 1.0 μg MIP-1α or MIP-1β (▪) in PBS. The data presented are the... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology OVA-specific AFCs in spleen, nasal tract, lung, and intestinal lamina propria.Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■)or 1.0 μg MIP-1α (▨) or MIP-1β (▪) in PBS. Levels of OVA-specific AFCs present in sp... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology Proliferation responses by OVA-specific CD4+T cells from mice immunized with OVA plus MIP1α or MIP-1β.Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■)or 1.0 μg MIP-1α (▨) or MIP-1β (▪) in PBS. One week after t... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology MIP-1α and MIP-1β differentially regulate Ag-specific Th cytokine responses.Groups of 5 C57BL/6 mice were intranasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (■) or 1.0 μg MIP-1α (▨) or MIP-1β (▪) in PBS. One week after the last immunization,... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology MIP-1α– and MIP-1β–mediated OVA-specific CTL responses.Groups of 5 C57BL/6 mice were nasally immunized on days 0, 7, and 14 with 75 μg OVA and 0.0 (▪) or 1.0 μg MIP-1α (♦) or MIP-1β (●) in PBS. One week after the last immunization, spleen-, Peyer patch–, an... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology Regulation of CD80, CD86, and CD40 expression by B220+ cells by MIP-1α and MIP1β.Splenocytes from DO11.10 mice were incubated with 0, 1, 10, 100, or 1000 ng/mL MIP-1α (triangles) or MIP-1β (squares) in plates containing 0 (open symbols) or 500 (filled symb... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology Regulation of CD28 and CD154 (CD40L) expression on CD4+ cells by MIP-1α and MIP-1β.Spleen cells from DO11.10 mice were incubated with 0, 1, 10, 100, or 1000 ng/mL MIP-1α (triangles) or MIP-1β (squares) in plates containing 0 (open symbols) or 500 (filled sy... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology Regulation of CD137 (4-1BB) expression on CD4+ and CD8+ cells by MIP-1α and MIP-1β.Spleen cells from DO11.10 mice were incubated with 0, 1, 10, 100, or 1000 ng/mL MIP-1α (triangles) or MIP-1β (squares) in plates containing 0 (open symbols) or 500 (filled sy... James W. Lillard, Jr et al. Blood 2003;101:807-814 ©2003 by American Society of Hematology