Download Microscope - Net Start Class

Our
Microscope
Photo by D. Tresten, 2002
6th Grade Science
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Eye piece
Magnify 10 X
Revolving
nosepiece
Objective
lens
Stage
Diaphragm
Light source
Base
Body tube
Arm
Stage clips
Coarse
adjustment
knob
Fine
adjustment
knob
Stage opening
Magnify 10x
To turn objectives (lenses)
into place over specimen
To magnify 40x
To magnify about 4X
To magnify about 10X
Allow more/less light
Support / Hold lenses
in place
Hold slide
Hold body
tube/Carry
Focus quickly but
imperfectly
(coarsely)
Send light up through
specimen
Focus very slowly more
perfectly (actually moves
the stage away from/toward
the objectives)
Support microscope
Allow light to reach
specimen / Catch spilled
matter
IMPORTANT!
Watch How Light Travels Through a Microscope
Note: Just study the vertical (up and down) portion of the light beam!
The horizontal (right/left) portion is not in our microscope.
Molecular Expressions Microscopy
Primer: Anatomy of the Microscope
- Transmitted Light Microscopy
Optical Pathways: Interactive Java
Tutorial
Which type of lens is in a microscope?
• Convex Lens
Light waves
See a convex lens
here:
http://en.wikipedia.
Check
out how a convex lens
org/wiki/Lens_(opti
works in your eyes! Molecular
cs) Microscopy
Expressions
(Pooches out in the middle!)
http://en.wikipedia.org/wiki/Image:Lens _and_w avefro nts.
gif
• Concave Lens
Primer: Light and Color Human Vision: Interactive Java
Tutorial
(Caves in in the middle!)
See how a concave lens
works: Molecular
Expressions: Light and Color
- Bi-Concave Lenses:
Interactive Java Tutorial
In Which Objects would Convex
Lenses be Used?
Click to see
7 images
In Which Objects might Concave
Lenses be Used?
Click to see
7 images
Total Magnification What is it?
To find the total magnification at which a microscope
is set, just multiply the power of the eyepiece (10x)
times the power of the objective that is in place.
Click to practice finding total magnification…
Practice calculating
Total Magnification:
If the eye piece is 10 x and the
objective lens is 40 x the
magnificaiton is...
400x
If the eye piece is 10 x and the
objective lens is 20 x the
magnificaiton is...
200x
If the eye piece is 10 x and the
objective lens is 10 x the
magnificaiton is...
100x
How do you take care of a
microscope?
Care of the Microscope,
Always carry a microscope
two
with _______
hands, one on
arm and the other on
the ______
base
the ________.
lens
Clean lenses with ______
paper
_________
only.
lenses
NEVER touch _________
with finger tips.
Care of the Microscope
Page 3 of 3
away
Always keep the scope _____
edge of the
from the _______
table
_________.
How to Use the Microscope
Before using the Scope,
Wake It Up:
1. STAGE down! (away from objectives)
2. Twist 4x (Low-Power) OBJECTIVE until it
clicks into place! (using nosepiece NOT objectives)
3. Stage CLIPS out!
4. SLIDE on
5. Stage CLIPS back in (carefully placed on top
of slide)
6. Set disc diaphragm to smallest hole
7. Switch on LAMP
Use of the Microscope
Page 1
1. Make sure
that the
stage is all
the way
DOWN
and
In place
slide is in
place.
Down
2.Set Disc
Diaphragm to
“Least Light”
(Setting 5 or 1)
Close-up of
disc
diaphragm
(as seen
from
underneath
the stage)
Do you see
the largest
hole that
lets the
most light
pass?
Which hole
transmits
the least
light?
3. Set Scope to Low Power
(100x)
In other
words, set
the 10x
objective in
place over
the stage
opening.
10x Objective
4. Looking from the side, use the
coarse adjustment knob to raise the
stage
as close as
possible to the
objectives…
5. Looking through the eyepiece for
the first time (with both eyes open),
use the fine
adjustment
knob until
the specimen
is in sharp,
clear
focus.
Use of the Microscope
Page 3
How to Focus on a Specimen, Cont’d:
6. If there is too little light,
rotate the disc diaphragm
to another opening.
Note: If the
object is a blur
or just a shadow
(like this one is),
it is NOT in
focus. Keep
turning the fine
adjustment knob
until it is in
sharp focus.
What is this?
Pointer in
eyepiece
“Field of Vision” in a Microscope
Always put our microscopes
“to bed” after labs:
Use of the Microscope
Page 4
1. Lamp off
2. Unplug
3. Stage down (Away from objectives)
4. All slides are removed
5. Clips IN
6. Clean stage
7. Dust cover ON
8. Put all slides and other materials in proper places
Wet Mount with a
Squashed Air Bubble in It
How to Add More Water to
a Wet Mount without
Starting All Over Again!
Why Learn to Use a
Microscope?
To see cool things like anything
on the following web page!
http://www.molecularexpressions.com
/moviegallery/pondscum.html