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PowerPoint® Clicker Questions
prepared by Heather
Williamson Jordan, Ph.D.,
University of Tennessee,
Knoxville
CHAPTER
6
Microbial
Nutrition and
Growth
© 2014 Pearson Education, Inc.
An organism that uses light energy to form ATP
and can only grow on organic carbon sources is
called a ________.
a)
b)
c)
d)
chemoautotroph
photoautotroph
chemoheterotroph
photoheterotroph
© 2014 Pearson Education, Inc.
An organism that uses light energy to form ATP
and can only grow on organic carbon sources is
called a ________.
a)
b)
c)
d)
chemoautotroph
photoautotroph
chemoheterotroph
photoheterotroph
© 2014 Pearson Education, Inc.
What type of microbes must possess the
enzyme superoxide dismutase?
a)
b)
c)
d)
obligate aerobes
obligate anaerobes
thermophiles
psychrophiles
© 2014 Pearson Education, Inc.
What type of microbes must possess the
enzyme superoxide dismutase?
a)
b)
c)
d)
obligate aerobes
obligate anaerobes
thermophiles
psychrophiles
© 2014 Pearson Education, Inc.
What type of microorganisms would you find
living near hot springs?
a)
b)
c)
d)
barophiles
psychrophiles
mesophiles
thermophiles
© 2014 Pearson Education, Inc.
What type of microorganisms would you find
living near hot springs?
a)
b)
c)
d)
barophiles
psychrophiles
mesophiles
thermophiles
© 2014 Pearson Education, Inc.
What happens if a cell is placed in a hypertonic
solution?
a) Water rushes into the cell, causing it to swell.
b) Water leaves the cell, causing it to shrink.
c) The amount of water leaving the cell equals the
amount of water entering the cell.
d) Nothing happens to the cell.
© 2014 Pearson Education, Inc.
What happens if a cell is placed in a hypertonic
solution?
a) Water rushes into the cell, causing it to swell.
b) Water leaves the cell, causing it to shrink.
c) The amount of water leaving the cell equals the
amount of water entering the cell.
d) Nothing happens to the cell.
© 2014 Pearson Education, Inc.
Which of the following is NOT a benefit to
microbes living in a biofilm?
a) more choices for reproductive mates, thus more
genetic diversity
b) protection from antimicrobial drugs
c) division of duties among biofilm members
d) protection from ultraviolet radiation
© 2014 Pearson Education, Inc.
Which of the following is NOT a benefit to
microbes living in a biofilm?
a) more choices for reproductive mates, thus more
genetic diversity
b) protection from antimicrobial drugs
c) division of duties among biofilm members
d) protection from ultraviolet radiation
© 2014 Pearson Education, Inc.
Which of the following describes the best way to
collect a clinical specimen from the skin?
a) Gently pour microbial broth media over the area of
interest and collect it in a flask.
b) Use a scalpel to gently scrape some skin cells onto a
microscope slide.
c) Press a Petri dish containing microbiological media
directly onto the skin.
d) Brush a sterile swab across skin, being careful not to
contact other areas.
© 2014 Pearson Education, Inc.
Which of the following describes the best way to
collect a clinical specimen from the skin?
a) Gently pour microbial broth media over the area of
interest and collect it in a flask.
b) Use a scalpel to gently scrape some skin cells onto a
microscope slide.
c) Press a Petri dish containing microbiological media
directly onto the skin.
d) Brush a sterile swab across skin, being careful
not to contact other areas.
© 2014 Pearson Education, Inc.
What is the purpose of performing the streakplate technique?
a)
b)
c)
d)
to obtain isolated colonies of bacteria
to count the number of bacteria in a specimen
to get the agar to release nutrients
to increase the number of colony-forming units
present in a specimen
© 2014 Pearson Education, Inc.
What is the purpose of performing the streakplate technique?
a)
b)
c)
d)
to obtain isolated colonies of bacteria
to count the number of bacteria in a specimen
to get the agar to release nutrients
to increase the number of colony-forming units
present in a specimen
© 2014 Pearson Education, Inc.
What must be done to a sample before
performing the pour-plate technique?
a) Broth culture must be added to the sample.
b) Colony-forming units must be separated by
performing serial dilutions.
c) The sample must be heated to 37 C.
d) An inoculating loop must be used to spread small
amounts of the sample across the surface of a solid
medium.
© 2014 Pearson Education, Inc.
What must be done to a sample before
performing the pour-plate technique?
a) Broth culture must be added to the sample.
b) Colony-forming units must be separated by
performing serial dilutions.
c) The sample must be heated to 37 C.
d) An inoculating loop must be used to spread small
amounts of the sample across the surface of a solid
medium.
© 2014 Pearson Education, Inc.
What is the best method for storing bacterial
cultures for short periods of time?
a)
b)
c)
d)
refrigeration
deep-freezing
dehydration
lyophilization
© 2014 Pearson Education, Inc.
What is the best method for storing bacterial
cultures for short periods of time?
a)
b)
c)
d)
refrigeration
deep-freezing
dehydration
lyophilization
© 2014 Pearson Education, Inc.
What is the best method of preservation to use
if you want to preserve a bacterial culture for 10
years or longer?
a)
b)
c)
d)
pressure chamber
refrigeration
deep-freezing
lyophilization
© 2014 Pearson Education, Inc.
What is the best method of preservation to use
if you want to preserve a bacterial culture for 10
years or longer?
a)
b)
c)
d)
pressure chamber
refrigeration
deep-freezing
lyophilization
© 2014 Pearson Education, Inc.
Which of the following statements is FALSE?
a) All known prokaryotes have been successfully
cultured.
b) The majority of prokaryotes have NOT been
successfully grown in culture medium.
c) Some microbes have very specific nutrient
requirements.
d) Some microbes can be grown in a variety of media.
© 2014 Pearson Education, Inc.
Which of the following statements is FALSE?
a) All known prokaryotes have been successfully
cultured.
b) The majority of prokaryotes have NOT been
successfully grown in culture medium.
c) Some microbes have very specific nutrient
requirements.
d) Some microbes can be grown in a variety of media.
© 2014 Pearson Education, Inc.
What is one disadvantage of complex media as
compared with defined media?
a) Organisms grow poorly on complex media.
b) Complex media are difficult to prepare.
c) The specific nutritional requirements of an organism
cannot be determined with complex media.
d) There are no disadvantages to using complex media.
© 2014 Pearson Education, Inc.
What is one disadvantage of complex media as
compared with defined media?
a) Organisms grow poorly on complex media.
b) Complex media are difficult to prepare.
c) The specific nutritional requirements of an
organism cannot be determined with complex
media.
d) There are no disadvantages to using complex media.
© 2014 Pearson Education, Inc.
Which of the following is NOT something that
can be used in differential media to help
distinguish between different kinds of bacteria?
a)
b)
c)
d)
blood
crystal violet
pH-sensitive dye
different carbon sources
© 2014 Pearson Education, Inc.
Which of the following is NOT something that
can be used in differential media to help
distinguish between different kinds of bacteria?
a)
b)
c)
d)
blood
crystal violet
pH-sensitive dye
different carbon sources
© 2014 Pearson Education, Inc.
What type of medium would you use to culture
an obligate anaerobe?
a)
b)
c)
d)
reducing medium
oxidizing medium
transport medium
selective medium
© 2014 Pearson Education, Inc.
What type of medium would you use to culture
an obligate anaerobe?
a)
b)
c)
d)
reducing medium
oxidizing medium
transport medium
selective medium
© 2014 Pearson Education, Inc.
How would you culture an organism that grows
best in 5% carbon dioxide?
a)
b)
c)
d)
This organism could not be cultured.
candle jar
cell culture
anaerobic culture system
© 2014 Pearson Education, Inc.
How would you culture an organism that grows
best in 5% carbon dioxide?
a)
b)
c)
d)
This organism could not be cultured.
candle jar
cell culture
anaerobic culture system
© 2014 Pearson Education, Inc.
What is binary fission?
a)
b)
c)
d)
the exchange of DNA between two cells
a way that some organisms create energy
the fusion of two cells to create a new, larger cell
a process in which a cell grows and divides to
produce two new daughter cells
© 2014 Pearson Education, Inc.
What is binary fission?
a)
b)
c)
d)
the exchange of DNA between two cells
a way that some organisms create energy
the fusion of two cells to create a new, larger cell
a process in which a cell grows and divides to
produce two new daughter cells
© 2014 Pearson Education, Inc.
Imagine that a certain bacterial species divides
by binary fission once every 30 minutes. If you
started with a culture containing 2 cells, how
many would be present after 2 hours?
a)
b)
c)
d)
4 cells
8 cells
16 cells
32 cells
© 2014 Pearson Education, Inc.
Imagine that a certain bacterial species divides
by binary fission once every 30 minutes. If you
started with a culture containing 2 cells, how
many would be present after 2 hours?
a)
b)
c)
d)
4 cells
8 cells
16 cells
32 cells
© 2014 Pearson Education, Inc.
During which phase of microbial growth is DNA
and protein synthesis maximal?
a)
b)
c)
d)
lag phase
log phase
stationary phase
death phase
© 2014 Pearson Education, Inc.
During which phase of microbial growth is DNA
and protein synthesis maximal?
a)
b)
c)
d)
lag phase
log phase
stationary phase
death phase
© 2014 Pearson Education, Inc.
When culturing a population of bacterial cells,
how can the stationary phase be avoided?
a)
b)
c)
d)
by the use of anaerobic jar
by the use of broth culture
by the use of a chemostat
The stationary phase cannot be avoided.
© 2014 Pearson Education, Inc.
When culturing a population of bacterial cells,
how can the stationary phase be avoided?
a)
b)
c)
d)
by the use of anaerobic jar
by the use of broth culture
by the use of a chemostat
The stationary phase cannot be avoided.
© 2014 Pearson Education, Inc.
An organism was grown in two different media:
Medium A and Medium B. When you compared
the growth curves of the two media, you saw
that the slope of the line in log phase was
steeper in Medium A than in Medium B. What
can you conclude?
a)
b)
c)
d)
The organism grows faster in Medium A.
The organism grows faster in Medium B.
The organism grows equally well in both media.
The organism grows only in Medium A.
© 2014 Pearson Education, Inc.
An organism was grown in two different media:
Medium A and Medium B. When you compared
the growth curves of the two media, you saw
that the slope of the line in log phase was
steeper in Medium A than in Medium B. What
can you conclude?
a)
b)
c)
d)
The organism grows faster in Medium A.
The organism grows faster in Medium B.
The organism grows equally well in both media.
The organism grows only in Medium A.
© 2014 Pearson Education, Inc.
Which of the following is NOT a direct method
for measuring microbial growth?
a)
b)
c)
d)
microscopic counts
membrane filtration
viable plate count
turbidity
© 2014 Pearson Education, Inc.
Which of the following is NOT a direct method
for measuring microbial growth?
a)
b)
c)
d)
microscopic counts
membrane filtration
viable plate count
turbidity
© 2014 Pearson Education, Inc.
What is the best way to measure microbial
growth if the population of microbes in a sample
is very small?
a)
b)
c)
d)
viable plate count
membrane filtration
dry weight
metabolic activity
© 2014 Pearson Education, Inc.
What is the best way to measure microbial
growth if the population of microbes in a sample
is very small?
a)
b)
c)
d)
viable plate count
membrane filtration
dry weight
metabolic activity
© 2014 Pearson Education, Inc.
What scientific instrument is used to measure
the turbidity of a microbial population?
a)
b)
c)
d)
Petroff-Hauser counting chamber
Coulter counter
spectrophotometer
flow cytometer
© 2014 Pearson Education, Inc.
What scientific instrument is used to measure
the turbidity of a microbial population?
a)
b)
c)
d)
Petroff-Hauser counting chamber
Coulter counter
spectrophotometer
flow cytometer
© 2014 Pearson Education, Inc.
Which of the following conditions will NOT
influence the accuracy of a viable plate count?
a)
b)
c)
d)
choice of plating medium
mixing of dilutions
growth phase of the bacterial population
size of the bacterial cells being counted
© 2014 Pearson Education, Inc.
Which of the following conditions will NOT
influence the accuracy of a viable plate count?
a)
b)
c)
d)
choice of plating medium
mixing of dilutions
growth phase of the bacterial population
size of the bacterial cells being counted
© 2014 Pearson Education, Inc.
What scientific device is useful for counting
larger cells such as protozoa?
a)
b)
c)
d)
Petroff-Hauser counting chamber
Coulter counter
spectrophotometer
flow cytometer
© 2014 Pearson Education, Inc.
What scientific device is useful for counting
larger cells such as protozoa?
a)
b)
c)
d)
Petroff-Hauser counting chamber
Coulter counter
spectrophotometer
flow cytometer
© 2014 Pearson Education, Inc.
If an organism cannot be grown in culture, how
can it be identified?
a)
b)
c)
d)
Gram staining
DNA testing
biochemical testing
aseptic techniques
© 2014 Pearson Education, Inc.
If an organism cannot be grown in culture, how
can it be identified?
a)
b)
c)
d)
Gram staining
DNA testing
biochemical testing
aseptic techniques
© 2014 Pearson Education, Inc.