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Diffusion and Osmosis Transport- Passive or Active • Passive transport-the movement of materials across a cell membrane without the expenditure of cell energy • Active transport-the use of cell energy to move materials across a cell membrane (against concentration gradient) PASSIVE TRANSPORT • From high to low concentration • Does not require energy from the cell – Diffusion – Osmosis – Facilitated diffusion ACTIVE TRANSPORT • From low to high concentration • Requires energy from the cell – Active transport proteins – Endocytosis – Exocytosis Diffusion & Osmosis DIFFUSION • The NET movement of particles from an area of high concentration to an area of low concentration Q: What is a concentration gradient?? What is a concentration gradient? • Concentration Gradient the difference in concentration of solutes in a given space • Diffusion happens when substances move “down” a concentration gradient (from high to low) until the concentration of the two areas becomes equal. • http://www.biologycorner.com/bio1/diffusion.html Dynamic Equilibrium • At dynamic equilibrium there is no NET movement of particles. • DYNAMIC implies movement • EQUILIBRIUM implies a balance – No longer a concentration gradient – Particles move equally in all directions Dynamic Equilibrium NOTE: At D.E. particles are still moving, its just that overall, they will remain “mixed” unless acted upon by another force. (So no “net” movement overall). Diffusion Diffusion will always be from an area of HIGH concentration to an area of ___________ LOW ____________ concentration. DYNAMIC It will continue until _______________ EQUILIBRIUM is reached. _______________ Diffusion OSMOSIS • The diffusion of WATER across a semipermeable membrane. http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html Osmosis Demos • http://www.biologycorner.com/bio1/diffusion.html # • http://www.emc.maricopa.edu/faculty/farabee/BI OBK/BioBooktransp.html What is the difference between diffusion and osmosis? • Diffusion is the NET movement of particles from an area of high concentration to an area of low concentration!” • Osmosis is just a “special case of diffusion”. It is the diffusion of water across a selectively permeable membrane. • Sometimes we refer to this movement as being from an area of HIGH water potential to an area of LOW water potential. But What is “WATER POTENTIAL”?? • Water potential is the measure of the ability of water to move (always from high to low) . It is the free energy of water in a system. • Think of osmosis as the net movement of water (remember water is also just a particle) down its concentration gradient. Diffusion occurs because particles move DOWN a concentration gradient. From high to low. Q: How large would the concentration gradient be at Dynamic Equilibrium? What is the cause of diffusion? • Diffusion happens because of the random kinetic energy (energy of motion) of particles and the existence of a concentration gradient. • All matter is made of particles and those particles are constantly in motion. What determines how fast diffusion occurs? • WHAT AFFECTS THE RATE OF DIFFUSION IN CELLS?? 1. Temperature 2. Charge 3. Diameter (Size) of particle 4. Concentration gradient (difference in concentration) 5. Surface Area of membrane Can We Control Diffusion? • Diffusion is a physical process. • It happens in both living and non-living systems. • We can’t control it because it is a natural process. • The cell sometimes needs to use energy to reverse the natural process of diffusion Q: What is this called?? Q: What type of cell transport is this? Inside Cell Outside Cell