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How can you tell which kitten is Rainbows clone? Explain the use of DNA. You can tell which kitten is rainbows clone because it has the exact same genetic make up as its mother, which in this instance is shown as 4 colours in a sequence. Does the clone have to look the same as Rainbow? Explain. No, while the embryo develops the genes responsible for the fur colour can be switched on and off at random to create different fur patterns. This is called X-linked Inactivation. Also genes and environment both have an effect on the fetus while it is growing. This can cause changes in personality and fur colour. Isolate the donor cells from Megdo, the Mother, and Mimi the Father Egg Cell Somatic Cell Remove and discard the nucleus from the egg cell Pipette to suck out Nucleaus Transfer the somatic cell nucleus into the enucleated egg cell. They will need some time to adjust to each other, this usually takes a couple of hours for the nucleus to be reprogrammed Enucleated Somatic N N Stimulate Cell Division, this is needed so the cell can begin mitosis. You need a chemical that mimics the actions of the male sperm cell, which isn't actually called Divide-a-Lot surprisingly. Divide-A-Lot Implant the embryo in Momi, the surrogate mother Deliver the baby mouse clone of Mimi