Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Powerpoint - Blood Journal
Document related concepts
no text concepts found
Transcript
Isolation and characterization of plasmacytoid dendritic cells from Flt3 ligand and granulocyte-macrophage colonystimulating factor–treated mice by Pia Björck Blood Volume 98(13):3520-3526 December 15, 2001 ©2001 by American Society of Hematology Elevated levels of IFN-α in Flt3L-treated mice.Mobilization of mice with Flt3L results in elevated levels of IFN-α2 production from (A) BM and (B) spleen cell populations. Pia Björck Blood 2001;98:3520-3526 ©2001 by American Society of Hematology Murine IFN-α–producing cells are present in the lymphoid, CD11c+CD11b− DC population.(A) Myeloid (CD11c+CD11b+) and lymphoid (CD11c+CD11b−) DCs were sorted from spleens of mice mobilized for 7 days with Flt3L+GM-CSF using the directly conjugated CD11c-PE an... Pia Björck Blood 2001;98:3520-3526 ©2001 by American Society of Hematology Major murine IFN-α–producing DC subset expresses a CD11clow, B220+ phenotype and lacks CD8α expression.(A) Staining of total splenocytes derived from Flt3L+GM-CSF–treated mice show that, in addition to myeloid CD11c+CD11b+ DC, a subset of CD11c+DC coexpress... Pia Björck Blood 2001;98:3520-3526 ©2001 by American Society of Hematology Production of IL-12 (p40) by DC subsets.Total CD11c+ DCs separated by directly conjugated magnetic beads were stimulated for 24 hours with stimulatory anti-CD40 antibodies (CD40 mAb), LPS (10 μg/mL), or SAC. For the detection of intracellular IL-12, DCs wer... Pia Björck Blood 2001;98:3520-3526 ©2001 by American Society of Hematology FACS profiles showing key markers for isolation of murine plasmacytoid, lymphoid, and myeloid DCs.Myeloid DCs were obtained by changing the settings for the FACS sorting so that CD11c+CD8α+ cells were excluded and CD11c+CD11b+ were included. Pia Björck Blood 2001;98:3520-3526 ©2001 by American Society of Hematology IFN-α–producing plasmacytoid cells differentiate into DCs after culture in IL-3.Cytospins of sorted CD11c+B220+ cells were stained using antibodies to MHC class II, a biotinylated goat anti–rat IgG antibody, and then by streptavidin-alkaline phosphatase and... Pia Björck Blood 2001;98:3520-3526 ©2001 by American Society of Hematology
