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Leaf Structure and Gas exchange in plants ORGANS of Flowering plants • • • • • Leaves Stems Roots Flowers Fruits Various Inputs and Outputs Water and mineral ions Some need to be moved around the plant PLANT REQUIREMENTS • GASES: – CARBON DIOXIDE: for photosynthesis – OXYGEN: for respiration • WATER: – As a reactant in photosynthesis – For support(turgor pressure) • MINERAL IONS – For making compounds required by the plant e.g amino acids, chlorophyll Getting what it needs for photosynthesis • Most photosynthesis occurs in the leaves (see next slide for labels) Leaf Cross Section: Anatomy of a leaf cuticle Upper epidermis palisade mesophyll Vascular bundle Xylem Phloem spongy mesophyll Air space Lower epidermis Guard cells cuticle stoma During the day Rate of photosynthesis > rate of respiration • CO2 is being used up in Photosynthesis by mesophyll cells, lowering its concentration here • CO2 diffuses from environment via open stomata into airspace above the stoma then into mesophyll cells • Movement is PASSSIVE; along a CO2 concentration gradient During the day Rate of photosynthesis > rate of respiration • MORE O2 is being produced in Photosynthesis than is used in respiration • O2 concentration in airspace in leaf increases • O2 diffuses from the airspace above the stoma, through the open stomata into the environment • Movement is PASSSIVE; along a O2 concentration gradient During the day • Water is constantly used in photosynthesis • Water is constantly evaporating from moist cell walls of leaf cells(transpiration) • This creates a high water vapour concentration in the airspaces in the leaf • Water (as a gas) diffuses out of the leaf via open stomata along a water concentration gradient • The rate of diffusion depends of the size of the gradient. Day time exchange from the leaf of a plant Xylem cuticle Upper epidermis Palisade cells chloroplasts Spongy cells stoma lower epidermis stoma Guard cell phloem Liquid water Water vapour Carbon dioxide oxygen Stomata • Opening in the epidermis • Mainly on lower surface of leaf Stomata • Formed by two guard cells • Guard cell contain chloroplast • Guard cells are sausage shaped Guard Cells are Special • They have chloroplast(unlike the other cells in the epidermis) • They have a cell wall that is thicker on the side adjacent to the pore than on the other side • The have radially orientated microfibrils that limit expansion of these cells in girth • Two guard cells are anchored to each other at their end Because of their structure when guard cells fill with water and become turgid they bend into a sausage shape and a pore forms between them Opening and Closing of Stoma 1. Solute uptake(mostly K+) into guard cells: 1. Solute loss from guard cells (mostly K+): 2. Water follows by osmosis 2. Water follows by osmosis 3.Guard cells expand, become turgid and bend 3. Guard cells become flaccid and change shape 5. Stoma OPENS 4 Stoma CLOSES H2 O H2 O H2 O H2 O K+ H2 O H2 O H2 O H2 O H2 O H2 O Stomata • Usually open in morning • Usually close at night • Also close to limit water loss Guard cells: located in the epidermis Some plant species have adaptation to arid conditions • Thick waxy cuticle: reduces evaporation of water across leaf surface • Sunken stomata(stomata in pits) or “hairy” leaves: Both strategies trap water vapour close to the leaf surface and reduce water vapour gradient • Inverted stomatal rhythm: stomata that open at night and close during the day, to reduce evaporative water loss in the hottest parts of the day Some plant species have adaptation to arid conditions • Thick Succulent leaves that can store water; also have a relatively small surface area over which transpiration can occur Agave Leaf Cross Section Saguaro Cactus- Gas Exchange in non woody stems • Diffusion from environment via stomata Gas Exchange in Woody Stems • Gas exchange occurs through Lenticels • Lenticels are spongy areas in corky surface of woody stems that allow gas exchange Gas exchange in Roots • Oxygen diffuses from air spaces in the soil into root hair cells and root cells • Carbon dioxide diffuses out in the opposite direction