Download Licence No: DIR 050/2004 - Office of the Gene Technology Regulator

Licence for dealings involving the
intentional release of a GMO into the
environment
Licence No: DIR 050/2004
Licence Holder: Queensland Department of Primary Industries
and Fisheries
Title:
Vaccination of cattle with recombinant bovine herpes
virus vaccines
Issued: 26 April 2005
Varied: 2 November 2007
More information about this licence is contained in the risk assessment and risk
management plan prepared in connection with this licence. This document can be
obtained from the Office of the Gene Technology Regulator website at
http://www.ogtr.gov.au or by telephoning the Office on 1800 181 030.
Gene Technology Regulation in Australia
Australia’s gene technology regulatory system operates as part of an integrated legislative
framework. The Gene Technology Act 2000 (Cth) and corresponding state and territory
legislation form a substantial part of a nationally consistent regulatory system controlling the
development and use of genetically modified organisms (GMOs).
The Gene Technology Regulator is required to consult with, and take into account advice
from a range of key stakeholders, including regulatory authorities, on risks to human health
and safety and to the environment in assessing applications for dealings involving the
intentional release of GMOs into the Australian environment.
Other agencies that also regulate GMOs or GM products include Food Standards Australia
New Zealand, Australian Pesticide and Veterinary Medicine Authority, Therapeutic Goods
Administration, National Industrial Chemicals Notification and Assessment Scheme,
National Health and Medical Research Council and Australian Quarantine and Inspection
Service. Dealings conducted under any licence issued by the Regulator may also be subject to
regulation by one or more of these agencies. It is recommended that the licence holder
consult the relevant agency (or agencies) about their regulatory requirements.
Dealings permitted by this licence may also be subject to the operation of State legislation
declaring areas to be GM, GM free, or both, for marketing purposes.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
2
SECTION 1
INTERPRETATION AND DEFINITIONS
This licence does not authorise dealings with GMOs that are otherwise prohibited as a result
of the operation of State legislation declaring areas to be GM, GM free, or both, for
marketing purposes.
In this licence:
(a) Words and phrases used in this licence have the same meaning as they do in the Act
and the Regulations;
(b) Words importing a gender include any other gender;
(c) Words in the singular include the plural and words in the plural include the
singular;
(d) Words importing persons include a partnership and a body whether corporate or
otherwise;
(e) References to any statute or other legislation (whether primary or subordinate) are a
reference to a statute or other legislation of the Commonwealth of Australia as
amended or replaced from time to time and equivalent provisions, if any, in
corresponding State law, unless the contrary intention appears;
(f) Where any word or phrase is given a defined meaning, any other part of speech or
other grammatical form in respect of that word has a corresponding meaning;
(g) Specific conditions prevail over standard conditions to the extent of any
inconsistency.
In this licence:
‘Act’ means the Gene Technology Act 2000 (Cth) and equivalent provisions in
corresponding State law;
‘Annual Report’ means a written report provided to the Regulator within 90 days of
each anniversary of this licence containing all the information required by this licence
to be provided in the Annual Report;
‘Decontamination’ means the application of the Decontamination Protocol found at
Attachment C so as to render the GMOs non-viable;
‘Destroy’, (or ‘Destroyed’ or ‘Destruction’) means, unless a condition prescribes the
manner of destruction, killed by one or more of the following methods:
(a)
incineration in an EPA approved incinerator; or
(b)
autoclaving; or
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3
(c)
only in relation to euthanased cattle, deep burial at EPA approved deep burial
quarantine site.
‘Equipment’ includes vehicles, clothing and tools;
‘GM’ means genetically modified;
‘GMOs’ means the genetically modified organism or organisms authorised for release
by this licence;
‘Location’ means both Location A and Location B and the Post-mortem Room.
‘Location A’ means the Queensland Department of Primary Industries and Fisheries’
PC1 Animal Containment Facility at the Animal Research Institute in Yeerongpilly
‘Location B’ means any fenced paddock located within the Queensland Department of
Primary Industries and Fisheries’ Animal Research Institute in Yeerongpilly
‘Material from Cattle’ means any organ or tissue from cattle vaccinated with the
GMOs.
‘OGTR’ means the Office of the Gene Technology Regulator;
‘Post-mortem Room’ means a facility within the Location where vaccinated cattle are
euthanased.
‘Regulator’ means the Gene Technology Regulator;
‘Sentinel Animal’ means an unvaccinated animal used in the dealing to test the ability
of the GMOs to be transmitted.
‘Shedding’ means the excretion by cattle of recombinant viral particles.
‘Sign-off’ means a notice in writing from the Regulator that no further testing is
required in respect of the Location or other areas used in connection with this licence.
‘Susceptible Animal’ means an animal that can be infected by the GMOs.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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SECTION 2
GENERAL CONDITIONS
Duration of Licence
1. This licence remains in force until it is suspended, cancelled or surrendered. No dealings
with GMOs are authorised during any period of suspension.
Holder of Licence
2. The holder of this licence (‘the licence holder’) is the Queensland Department of Primary
Industries and Fisheries.
Project Supervisor
3. The Project Supervisor in respect of this Licence is identified at Attachment A.
4. The licence holder must immediately notify the Regulator in writing if any of the contact
details of the Project Supervisor change.
No dealings with GMOs except as authorised by this licence
5. Persons covered by this licence must not deal with the GMOs except as expressly
permitted by this licence.
Persons covered by this GMO licence
6. The persons covered by this licence are the licence holder and employees, agents or
contractors of the licence holder and other persons who are, or have been, engaged to
undertake any activity in connection with the GMOs authorised by this Licence.
Informing people of their obligations
7. The licence holder must inform any person covered by this licence, to whom a particular
condition of this licence applies, of the following:
(a) the particular condition (including any variations of it);
(b) the cancellation or suspension of the licence;
(c) the surrender of the licence.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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8. If a particular condition, including any variation of it, applies to a person with respect to a
particular dealing, the licence holder must not permit a person covered by this licence to
conduct that dealing unless:
(a) the person has been informed of the condition, including any variation of it, and
(b) the licence holder has obtained from the person a signed and dated statement that the
person,
i. has been informed by the licence holder of the condition and, when applicable,
its variation; and,
ii. has understood and agreed to be bound by the condition, or its variation, and
has not conducted the dealing without being informed of the condition, or its
variation.
9. The licence holder must provide the Regulator, on the Regulator’s written request, with
copies of the signed and dated statements referred to in the immediately preceding
condition.
Licence holder to notify of circumstances that might affect suitability
10. The licence holder must immediately, by notice in writing, inform the Regulator of:
(a) any relevant conviction of the licence holder occurring after the commencement of
this licence;
(b) any revocation or suspension of a licence or permit held by the licence holder under a
law of the Australian Government, a State or a foreign country, being a law relating to
the health and safety of people or the environment;
(c) any event or circumstances occurring after the commencement of this licence that
would affect the capacity of the holder of this licence to meet the conditions in it.
Licence holder must provide requested information on matters related to suitability
11. The licence holder must provide information related to the licence holder’s ongoing
suitability to hold a licence when requested to do so in writing by the Regulator and must
provide the information within a time period stipulated by the Regulator.
Additional information to be given to the Regulator
12. It is a condition of a licence that the licence holder inform the Regulator if the licence
holder:
(a) becomes aware of additional information as to any risks to the health and safety of
people, or to the environment, associated with the dealings authorised by the licence;
or
(b) becomes aware of any contraventions of the licence by a person covered by the
licence; or
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(c) becomes aware of any unintended effects of the dealings authorised by the licence.
13. The licence holder must provide the information required by paragraphs (a) (b) and (c) of
the immediately preceding condition to the Regulator as soon as practically and
reasonably possible and must also include the information in the Annual Report.
People dealing with GMOs must allow auditing and monitoring of the dealing
14. If a person is authorised by this licence to deal with GMOs and a particular condition of
this licence applies to the dealing by that person, the person must allow the Regulator, or
a person authorised by the Regulator, to enter premises where the dealing is being
undertaken, for the purposes of auditing or monitoring the dealing.
Remaining an accredited organisation
15. The licence holder must, at all times, remain an accredited organisation in accordance
with the Act and comply with its instrument of accreditation.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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SECTION 3
SPECIFIC CONDITIONS
GMOs covered by this licence
16. The GMOs covered by this licence are described at Attachment B.
Permitted dealings
17. The permitted dealings with the GMOs are to vaccinate a population of cattle with the
GMOs and to test the safety and efficacy of the vaccine. The permitted dealings include
the possession, supply, use, transport and disposal of the GMOs for the purpose of any of
the permitted dealings with the GMOs, or in the course of any of these dealings.
Locations and size of trial
18. The permitted dealings with the GMOs, other than disposal of the GMOs, may only be
undertaken during the period 1 July 2005 to 31 April 2010.
19. The permitted dealings may only be conducted at the Location.
20. Up to 180 cattle may be selected for the dealings and each one may be vaccinated with a
GMO or any combination of GMOs.
21. Vaccination must occur within Location A.
22. The licence holder must be able to access and control a Location where the GMOs are
released to the extent necessary to comply with this licence, for the duration of the life of
the licence.
Notice of permitted dealings
23. The licence holder must provide a notice in writing to the Regulator each time cattle are
vaccinated with the GMOs at Location A.
24. The notice must set out:
(a) the date on which the cattle were vaccinated,
(b) the number of cattle vaccinated, and
(c) the GMO or GMOs used.
25. The notice must be provided to the Regulator no less than 30 days prior to
commencement of the date on which the cattle are vaccinated.
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Location B must be secured.
26. Paddocks holding vaccinated cattle within Location B must be securely fenced so as to
prevent the escape of the cattle from the paddock.
Persons at Location A or in the Post-mortem room must wear protective clothing
27. Boots, overalls and disposable gloves must be worn by any person who is handling
GMOs, or cattle that may contain the GMOs, in an area within Location A or in the Postmortem room. Boots, overalls and disposable gloves must be removed, and exposed areas
of skin must be washed before the person leaves Location A or the Post-mortem room.
28. In addition to the clothing required to be worn under the immediately preceding
condition, persons vaccinating the cattle at Location A must also wear a face mask until
vaccination has been completed.
29. During any period when Decontamination is occurring at Location A or in the Postmortem room the licence holder must ensure that persons not enter Location A or the
Post-mortem room for purposes of the dealings covered by this licence or for any other
purpose permitted by the licence holder unless clothed in boots and overalls and
disposable gloves.
30. At the conclusion of each trial, protective clothing must be decontaminated to destroy all
GMOs. If transported outside Location A or the Post-mortem room prior to
decontamination, contaminated protective equipment must be contained so as to prevent
dissemination of the GMOs.
Conditions about Susceptible Animals
31. No Susceptible Animals other than those involved in the dealing are to be held at the
Location unless approved by the Regulator, in writing.
32. Cattle that have been vaccinated with the GMOs must be clearly tagged to indicate that
they have been vaccinated with the GMOs.
33. Cattle that are shedding infectious GMOs must not be moved from Location A until 14
days after the period of shedding concludes.
34. Nothwithstanding the results of any research conducted under condition 60(c), cattle,
whether vaccinated with the GMO or not, must not be removed from the Location
without conclusive scientific evidence that the cattle are free of the GMOs at the time of
removal from the Location. The evidence must be provided to the Regulator on request.
Decontamination of Location A
35. The day after any of the cattle are vaccinated with the GMOs at Location A, the licence
holder must Decontaminate Location A and any Equipment used at Location A for
purposes of this licence.
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36. Following each vaccination, Decontamination at Location A must be repeated once every
day for 10 days.
37. Notice that the Decontamination has taken place must be provided to the Regulator
within 14 days of the date on which each ten day cycle of Decontamination has
concluded.
Decontamination of the Post-mortem Room
38. The Post-mortem room must be decontaminated immediately after each group of cattle
has been euthanased.
39. Notice that the Decontamination has taken place must be provided to the Regulator
within 14 days of its occurrence.
General conditions in relation to the Decontamination of all other places and
Equipment used in connection with this licence
40. If:
(a) an area or place other than the Location is used in connection with this licence; or
(b) Equipment is used in connection with the GMOs;
then that area, place or Equipment, must also be Decontaminated.
41. Decontamination must occur immediately or as soon as practicable after the use and
before the area or equipment, as applicable, is used for any other purpose.
42. Any areas in which Equipment is Decontaminated must also be Decontaminated.
43. On the request of the Regulator, the Regulator must be provided with written
documentation of the procedures in place to ensure continuing compliance with these
Decontamination conditions.
Testing for continuing presence of GMOs
After each Decontamination the licence holder must conduct tests capable of detecting any
continuing presence of viable GMOs at Location A and the post-mortem room and any other
area within the Location that has been decontaminated. Details of the testing regime must be
provided to the Regulator and approved prior to implementation.
44. If Location B is used to hold vaccinated cattle, tests must be conducted for the presence
of viable GMOs outside the cattle at Location B. Details of the testing regime including
suggested times for testing must be provided to the Regulator and approved prior to
implementation.
45. The results of testing activities must be recorded in a logbook. The logbook must be
available on request for examination or photocopying by the OGTR. The findings of the
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tests as recorded in the logbook must be included in the licence holder’s annual report to
the Regulator. The logbook must contain at least the following:
(a) details of the areas inspected;
(b) details of the date of inspection;
(c) whether GMOs were observed, and if so, the concentrations;
(d) any further Decontamination.
46. Any GMOs detected are to be treated as an unintended presence and the licence holder
must act in accordance with the Contingency Plan for unintended presence provided to
the Regulator under condition 56.
47. The licence holder must comply with any written direction of the Regulator to
Decontaminate an expanded area around the Location.
48. If following the final vaccination, no GMOs are detected by testing after
Decontamination, the licence holder may make written application to the Regulator for
Sign off.
Testing methodology
49. The licence holder must provide a written instrument to the Regulator describing the
experimental method to be used for detecting the presence of the GMOs and the presence
of the genetic modifications described in this licence (at Attachment B) in a recipient
organism. The instrument must be provided within 30 days of vaccination of the cattle.
Transportation of the GMOs and cattle
50. Subject to the conditions immediately below in respect of transportation, the GMOs, and
Material from Cattle must be transported in accordance to the OGTR Guidelines for the
Transport of GMOs as current at the time of transportation.
51. Every container used to transport the GMOs, or Material from Cattle must be labelled:
(a) to indicate that it contains GM BoHV-1; and
(b) with telephone contact numbers for the licence holder and instructions to contact the
licence holder in the event that the container is broken or misdirected.
Disposal of the GMOs and cattle
52. After the experiments have been conducted and before the expiration of this licence, all
cattle used in this dealing must be euthanased at the Location.
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53. Euthanased cattle must be Destroyed by either:
(a) incineration at an EPA-approved incinerator; or
(b) deep burial at an EPA-approved deep burial quarantine site.
Incineration or deep burial must occur on the same day as removal from the Location
occurred.
54. The cattle may be destroyed during, or at the completion of, experiments.
55. After the experiments have been conducted and before the expiration of this licence, all
GMOs must be destroyed by autoclaving or incineration, unless stored in accordance
with OGTR policy on storage of genetically modified organisms. GMOs may be
destroyed during or at the completion of experiments.
Contingency Plans
56. Within 30 days of the date of the commencement of this licence, a written Contingency
Plan must be submitted to the Regulator detailing measures to be taken in the event of the
unintended presence of the GMOs outside cattle that have been vaccinated with the
GMOs.
57. The Contingency Plan must include details of procedures to ensure the Regulator is
notified immediately if the licence holder becomes aware of the event and to
decontaminate the area
58. The Contingency Plan must be implemented in the event that the unintended presence of
the GMOs is discovered outside cattle that have been intentionally vaccinated with the
GMOs.
Compliance Management Plan
59. Prior to dealing with the GMOs a written Compliance Management Plan must be
provided to the Regulator. The Compliance Management Plan must describe in detail
how the licence holder intends to ensure compliance with these conditions and document
that compliance.
Research Requirements
60. During the period of this licence, the licence holder must test for the following in
consultation with the OGTR and provide the information to the Regulator either before or
during the trial or in the Annual Report:
(a) the presence, if any, of antibodies to BoHV-1 or GM BoHV-1 in workers who
both inoculate the trial animals and handle the inoculated cattle; and
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(b) the ability of the GM BoHV-1 to infect, replicate in and express transgenes on the
surface of cell lines from a variety of mammals including humans, sheep, goats,
rabbits, mice and monkeys in vitro. The details and results of these experiments
must be reported to the Regulator before in vivo experiments are carried out. If the
results of these experiments indicate that any of the GMOs are able to infect and
replicate in any of the cell lines, the GMO must not be used in the dealing unless
the Regulator is satisfied that there are no additional risks to human health and
safety or to the environment posed by the use of the GMO in in vivo trials; and
(c) the ability of the GMOs to be transmitted from vaccinated animals to Sentinel
Animals (cattle, sheep and goats) inside Location A and to Sentinel Animals
(cattle only) outside Location A must be investigated and the results made
available to the Regulator. Once information regarding the ability of the Sentinel
Animals to be infected with the GMOs has been gathered, all Sentinel animals
must be disposed of in the same way as vaccinated animals; and
(d) any reactivation of the virus in trial animals during resting periods in paddocks
pursuant to animal ethics considerations prior to euthanasia; and
(e) the persistence and stability of the GM BoHV-1 in field conditions; and
(f) residual levels of introduced gene products in different animal tissues, as advised
by the Regulator.
61. A progress report on the research conducted under the previous condition must be
provided to the Regulator in the Annual Report.
Reporting
62. The licence holder must provide an Annual Report to the Regulator.
GMOs must not be consumed
63. The licence holder must ensure that the GMOs, animals vaccinated with the GMOs,
Material from Cattle are not consumed by humans or animals.
Animal ethics approval
64. The dealings authorised by this licence involving the use of animals must not commence
unless the Animal Ethics Committee for the Queensland Department of Primary
Industries and Fisheries has first considered and consented in writing to the performance
of the dealings.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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Attachment A
DIR No: 050/2004
*Full Title
Vaccination of cattle with recombinant Bovine Herpesvirus Vaccines
Organisation Details
Postal address:
AFFS
Biotechnology, Level 6, North Tower, QBP
306 Carmody Road,
ST. LUCIA QLD 4072
Phone No:
[Personal information redacted]
Fax No:
[Personal information redacted]
Project Supervisor Details
Surname:
[Personal information redacted]
First name:
[Personal information redacted]
Title:
[Personal information redacted]
Phone No:
[Personal information redacted]
Email:
[Personal information redacted]
Position:
[Personal information redacted]
Organisation:
Queensland Department of Primary Industries and Fisheries
Postal address:
AFFS
Biotechnology, Level 6, North Tower, QBP
306 Carmody Road,
ST. LUCIA QLD 4072
IBC Details
IBC Name:
Queensland Department of Primary Industries and Fisheries IBC
* Information that must be included in the Record of GM Products and GMOs Dealings.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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Attachment B
GMOs Description
*Parent Organism
Common name:
Bovine herpesvirus 1 (BoHV-1)
Scientific Name:
Bovine herpesvirus 1 subtype 1.2b, strain V155
*Modified Trait(s)
Category:
Reporter/ Marker gene expression, Antigen expression, Attenuation
Description:

Expression of green fluorescent protein (GFP)

Expression of envelope glycoprotein E2

Expression of a truncated E0 glycoprotein fused to GFP or to the
E2 glycoprotein

Localisation of introduced proteins on the surface of the GMOs or
host cells

Deletion or disruption of endogenous BoHV-1 genes
*Genes responsible for conferring the modified trait(s)

GFP gene from Aequorea victoria

synthetic E2 gene from Bovine viral diarrhoea virus (BVDV)

truncated E0 gene from BVDV fused in-frame to the GFP gene
from A. victoria or the synthetic E2 gene from BVDV

signal sequences from the E0/Erns gene from BVDV, from the Gglycoprotein gene of Bovine respiratory syncytial virus (BRSVG) and from native gC, gE, gJ and gK genes of BoHV-1

native BoHV-1 glycoprotein genes gC, gE, gJ and gK

native BoHV-1 thymidine kinase gene
* Information that must be included in the Record of GM Products and GMOs Dealings.
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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Details
The GMOs covered by this licence are as follows:
Table 1 GMOs for release
GMO
Promoter
1
Signal sequence
Gene inserted
Poly-Adenylation
sequence
hCMV-ie
GFP
RBGpA
2
hCMV-ie
E2syn
RBGpA
3
hCMV-ie
E0-GFP
RBGpA
4
hCMV-ie
E2syn + E0-GFP
RBGpA
5
hCMV-ie
GFP
RBGpA
6
hCMV-ie
GFP-E2syn
RBGpA
7
hCMV-ie
BRSV-G
GFP-E2syn
RBGpA
8
hCMV-ie
BVDV
GFP-E2syn-E0
RBGpA
9
hCMV-ie
BVDV
E2syn
RBGpA
10
hCMV-ie
BVDV
GFP-E2syn
RBGpA
11
hCMV-ie
BVDV
E2syn-E0
RBGpA
12
gC native
gC native
E2syn
gCpA
13
gE native
gE native
E2syn
gEpA
14
gJ native
gJ native
E2syn
gJpA
15
gK native
gK native
E2syn
gKpA
16
TK native
BVDV
E2syn
TKpA
17
hCMV-ie
BVDV
E2syn
RBGpA/ BGHpA
18
gE native
BVDV
E2syn
RBGpA/ BGHpA
19
hCMV-ie
BVDV
E2syn
RBGpA/ BGHpA
BRSV-G
Licence No. 050/2004, Issued 26 April 2005, Varied 2 November 2007
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Attachment C
Decontamination protocol
LARGE ANIMAL HOUSE PC1 FACILITY:
A.
Daily Decontamination procedure during vaccination trials:
1. Cattle will be removed from the holding pen
2. Pens will be liberally doused with a 1 % (v/v) solution of detergent. Large pieces of
faeces will be broken in to smaller pieces and the entire pat moved to ensure detergent
is able to contact all solid surfaces.
3. After 5 minutes the pen will be thoroughly washed with water.
4. All material washed from the pens will be held in a holding tank or adjacent drains
and retreated with detergent.
5. After 10 minutes the run off will be discharged in the Brisbane City Sewer system and
washed through with a large volume of water.
NOTE: This procedure will also be utilised to decontaminate the animal handling section
located in the central part of the PC1 facility.
B.
Decontamination procedure at completion of vaccination trials:
1. Cattle will be removed from the holding pen
2. Pens will be liberally doused with a 1 % (v/v) solution of detergent. Large pieces of
faeces will be broken in to smaller pieces and the entire pat moved to ensure detergent
is able to contact all solid surfaces.
3. After 10 minutes the pen will be thoroughly washed with water.
4. All material washed from the pens will be held in a holding tank or adjacent drains
and retreated with detergent.
5. After 10 minutes the run off will be discharged in the Brisbane City Sewer system and
washed through with a large volume of water.
6. Animal holding pens will then be treated with Virkon 5 for 10 minutes as described
by the manufacturer.
7. Pens will be thoroughly washed to completely removed Virkon 5.
NOTE: Due to the caustic nature of Virkon 5 it is not considered practical to use in daily
with animals in the PC1 facility.
POST-MORTEM ROOM:
1. Cattle will be euthanased.
2. Following dissection all cattle parts will be double bagged and placed in transport
containers. Any faeces will also be double bagged and disposed of as clinical waste.
3. The post-mortem room (including transport containers) will be liberally doused with a
1 % (v/v) solution of detergent. All instruments used in the dissection of the cattle
will be also treated with detergent. Where possible instruments will also be
autoclaved.
4. After 10 minutes the post-mortem room will be thoroughly washed with water.
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5. The post-mortem room will then be treated with Virkon 5 as described by the
manufacturer.
6. The post-mortem room will be thoroughly washed to completely removed Virkon 5.
7. Pathological waste bins removed to cold storage facility.
8. If large volumes of cattle material are being processed and it is necessary to remove
pathological waste bins from the post-mortem room prior to full decontamination as
described in Steps 1 to 6, the bins will be thoroughly doused in detergent and rinsed
prior to removal from the post-mortem room.
NOTE: Care will be taken to ensure decontamination of the wheels of the pathological waste
bins, the bottoms of the bins and the area of floor underneath the bins prior to removal from
the post-mortem room.
ANIMAL TRANSPORT VEHICLE:
1. Following the transport of all animals to the post-mortem room.
2. The animal holding cage will be liberally doused with a 1 % (v/v) solution of
detergent. Large pieces of faeces will be double bagged and placed in clinical waste
bins.
3. After 15 minutes the cage will be thoroughly washed with water.
4. The animal holding cage will then be treated with Virkon 5 as described by the
manufacturer.
5. The animal holding cage thoroughly washed to completely removed Virkon 5.
HOLDING PADDOCKS:
1.
2.
3.
4.
Following the removal and euthanasia of cattle in the post mortem room.
The paddock will be liberally doused with a 1 % (v/v) solution of detergent.
After 15 minutes the paddock will be thoroughly washed with water.
Repeat steps 1 to 3.
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