Download Additional file 5

Supplementary table 5. Primers used for the expression study and their main features.
% GC: percentage of G and C; AL: amplicon length (bp); Ta: annealing temperature (°C).
%
Primer
Sequence (5’ to 3’)
Gene (Accession number)
Tm
AQ-For
TCTCGGGCCCTTGTTTTAGA
Aquaporin (JQ711526)
58
50
AQ-Rev
AAAGAGAGGCCAGCAACCG
59
58
58
45
59
48
60
48
59
55
60
50
59
50
59
42
59
60
58
48
60
63
62
50
59
50
59
60
58
58
61
45
60
50
60
50
Beta-GLUFor
Beta-GLURev
TTGCCAAAACGTTCAGCTGT
Beta-glucosidase (AAL93619.1)
GCCTCTAAGCCTTTTACGACGAT
C4H-For
CGGCATTACTTTGGGACGTTT
C4H-Rev
GCAGACTGAATTGGCCACCT
Cinnamate 4 hydroxylase (JQ711532)
Serine-carboxypeptidase-like protein
Carbox For
GTGTCGCAAATGTGAGATGG
Carbox Rev
GATCATGACATGGCTGCACT
CAT-For
CCGAAAATGGGTAGATGCTTTATC
CAT-Rev
GAGGCGAGCTTCTGACCAAG
CHIT II-For
TCGACTCCATGAACATCATCG
CHIT II-Rev
GAGCCTGTTCGGCACAGGT
DisRes For
GCCATTCGGAAAACACCAGT
DisRes Rev
CTTTCAGCGGAAGCGTAATC
EF1-For
TGCACAGTTATTGATGCTCCA
EF1-Rev
GGGCTCCTGAATCTGGTCAA
Ethylen For
TCATGTGCGTTGTTGATGGT
Ethylen Rev
TGTCTTTTAACATTCACACAGA
GST-For
CTTTTCGATGAACGCCCTCA
(JQ711528)
Catalase (ABS72010.1)
Chitinase I (JN696113)
Disease resistance protein (JQ711509)
Elongation factor -1α (AM946404.1)
Ethylene Responsive Protein (JZ331728)
Glutathione-S-transferase (JQ711516)
GC
AL
Ta
91
56
110
58
101
59
160
60
110
58
111
58
160
60
400
58
161
60
91
59
GST-Rev
AACGCTTCGTCATTGCAACA
LOX-For
CGGGTTGGACCAGTGAATGT
LOX-Rev
TTGACACACTGTTGGGAATTCC
Metal-1 For
CCATAGCTACTTTGAAGGGCTTA
Metal-1 Rev
GTGGTCGCACCAAAGAAGAT
PR10-For
CAGGAGAAGCACCGTGACAA
PR10-Rev
TCTGAAGATCGAGAATGCTTTCC
PR27-For
CCCAGAAGATCAACGAAGTAGGTG
PR27-Rev
GACCGCACGATTCTTGGATT
SOD- For
TGGTGGAAGAGTTGCTTGTGG
SOD- Rev
TGATGACCGAGTTTGTACACAGTG
TCI-For
TGTTGTGATACGTGCCTTTGC
TCI-Rev
CATTTGTCGCATGCAGAGTGA
TPI II For
TGTGCAGCTTGGATGAAGCT
TPI II Rev
CAACGTGAACTATGCTGAAGCC
Transd For
GCTTGGAACCATGGAGAGAA
Transd Rev
TTTTCCAAAGAACCCACCAG
Ubiquitin
For
GTGGATCGTCTGGATTTGGA
Lipoxygenase (EU513351)
Metallothionein type 1 (JQ711520)
PR 10 (JQ711524)
PRp27 (JN696114)
Superoxide dismutase (JK784468)
Trypsin/chymotrypsin inhibitor
(JQ429797)
Tripsin protease inhibitor II (JQ429796)
Transducin (JQ711533)
Ubiquitin-coniugating enzyme (JQ429798)
Ubiquitin
Rev
TCCGGTTCCTCACCAAAATA
UP1-For
GCATTGGTCAGTATAATGCATCCT
UP1-Rev
GGAAGCAGCAACACTTTTTCAA
UP2-For
CGACGAACTTCACTCAGCCA
UP2-Rev
TCCGCCTTCTCTTCACTGATC
Unknown protein 1 (JQ711535)
Unknown protein 2 (JQ711536)
59
45
59
55
58
45
59
43
60
50
58
74
59
54
60
50
59
50
60
52
59
46
58
48
59
48
58
56
59
56
60
50
59
45
60
50
60
45
58
42
58
41
59
55
58
52
90
60
170
60
100
58
110
58
110
60
91
58
112
60
205
60
156
60
110
58
110
59