Download Biotechnology AS

July 2009
Florida Department of Education
Curriculum Framework
Program Title:
Occupational Area:
Career Cluster:
CIP Number:
Grade Level:
Standard Length:
I.
Biotechnology
Industrial Education
Manufacturing
PSV
1626061600 A.S.
0626061600 A.A.S.
College Credit
61 Credits
MAJOR CONCEPTS/CONTENT: This program is designed to prepare students
for employment as Biotechnology Research Technicians, Biological Technicians
(SOC Code 19-4021) or cell culture technicians or biotechnology manufacturing
technician and/or to supply supplemental training for persons previously or
currently employed in these occupation.
The program includes, but is not limited to, broad biology and chemistry
concepts, algebraic and statistical analysis, basic microbiology concepts,
biohazard and safety procedures, human anatomy and physiology or botany,
core biotechnical laboratory techniques and industry workplace experience. It
includes components designed to enhance critical thinking and technical
communication skills.
Reinforcement of basic skills in English, mathematics, and science appropriate
for the job preparatory programs occurs through classroom instruction and
applied laboratory procedures or practice.
This program focuses on broad, transferable skills and stresses understanding
and demonstration of the following elements of the health care industry;
planning, management, finance, technical and production skills, underlying
principles of technology, labor issues, community issues and health, safety, and
environmental issues.
II.
LABORATORY ACTIVITIES: Clinical experiences are provided and are an
integral part of the program. They may be in the form of clinical experience,
practica or internships.
III.
To be transferable statewide between institutions, this program/course must
have been reviewed and a "transfer value" assigned the curriculum content by
the appropriate Statewide Course Numbering System discipline committee. This
does not preclude institutions from developing specific program or course
articulation agreements with each other.
SCANS Competencies: Instructional strategies for this program must include
methods that require students to identify, organize, and use resources
appropriately; to work with each other cooperatively and productively; to acquire
and use information; to understand social, organizational, and technological
systems; and to work with a variety of tools and equipment. Instructional
strategies must also incorporate the methods to improve students' personal
qualities and higher-order thinking skills.
Federal and state legislation requires the provision of accommodations for
students with disabilities to meet individual needs and ensure equal access.
Adult students with disabilities must self-identify and request such services.
Students with disabilities may need accommodations in such areas as
instructional methods and materials, assignments and assessments, time
demands and schedules, learning environment, assistive technology and special
communication systems. Documentation of the accommodations requested and
provided should be maintained in a confidential file.
Equipment List: A generic equipment list is available for the program/cluster and
is printed in a supplement to this document.
The standard length of the program is credit hours.
IV.
INTENDED OUTCOMES:
01.0 Demonstrate communication skills.
02.0 Demonstrate safety skills.
03.0 Demonstrate basic laboratory skills.
04.0 Demonstrate regulatory compliance.
05.0 Demonstrate appropriate decision making and problem
solving techniques.
06.0 Demonstrate specific laboratory skills.
07.0 Demonstrate quality assurance/control.
08.0 Maintain facility & equipment
09.0 Demonstrate proper care/use of test animals/plants
Florida Department of Education
STUDENT PERFORMANCE STANDARDS
Program Title:
Postsecondary number:
01.0
Biotechnology
1626.061600 A.S. / 0626.061600 A.A.S.
DEMONSTRATE COMMUNICATION SKILLS -- The student will be able to:
01.01 Make professional oral & written presentations.
01.02 Comprehend and use correct technical vocabulary.
01.03 Follow/analyze protocol.
01.04 Keep accurate records.
01.05 Take notes on procedures.
01.06 Prepare identify & apply changes to control procedures.
01.07 Write or update manuals, SOP’s protocols, reports and technical
summaries.
01.08 Perform computerized research and web searches.
01.09 Read technical literature.
01.10 Identify basic reference resources in biotechnology.
01.11 Perform basic applications in word processing, spread sheets, databases,
presentations and project management.
01.12 Navigate the internet.
02.0
DEMONSTRATE SAFETY SKILLS -- The student will be able to:
02.01 Identify first aid supplies, co-worker contact, medical information, and
emergency protection and evacuation plan.
02.02 Follow correct safety procedures, guidelines and chemical hygiene plans.
02.03 Maintain required safety training.
02.04 Maintain a safe work area.
02.05 Maintain and utilize safety equipment & personal protection equipment.
02.06 Check expiration dates, lot numbers & labels for hazards.
02.07 Monitor usage and exposure of radioisotopes, & biohazards.
02.08 Handle, store and dispose of hazardous materials per MSDS, other safety
guidelines & Worker Protection Standards (WPS).
02.09 Follow universal precautions for biological pathogens.
02.10 Store chemicals and biologicals according to storage guidelines.
03.0
DEMONSTRATE BASIC LABORATORY SKILLS -- The student will be able to:
03.01 Obtain and read protocol, test procedure, standard operating procedure
(SOP) & proper forms.
03.02 Prioritize & perform multiple tasks in a timely manner.
03.03 Clean, organize and sterilize materials , when required.
03.04 Check and maintain equipment, logs & perform preventative maintenance
tasks according to schedule.
03.05 Order inventory of supplies; date/label reagents.
03.06 Practice aseptic technique.
03.07 Use titration/pipetting techniques; measure volume/weights.
03.08 Perform basic calculations and statistical analysis.
03.09 Calculate and prepare dilutions series.
03.10 Prepare solutions and reagents for laboratory use.
03.11 Monitor physical properties of reagents, buffers, media & solutions &
determine optimum conditions for use.
03.12 Obtain and review appropriate procedures & test forms.
03.13 Collect and set up samples for analysis.
03.14 Set up general laboratory tests, including, setup equipment &
perform/document tests and results.
03.15 Operate the following(but not limited to) equipment: Thermocyloer,
microscopes, fluorimeter, hoods, centrifuge, polarimeter, pH meter,
chart recorder, stirrers, balance, conductivity meter, mixers, autoclave,
power supply, shakers, dry heat ovens, incubator, Bunsen burner,
scintillation counter, high pressure liquid chromatography, gas
chromatography/mass spectrometry.
04.0
DEMONSTRATE REGULATORY COMPLIANCE -- The student will be able to:
04.01 Follow the guidelines from the following agencies: FDA, OSHA, USDA,
NIH, NR, DOT, EPA, CDC & NRC.
04.02 Accept state, local and industry regulations.
04.03 Perform manufacturing using current good manufacturing practices.
05.0
DEMONSTRATE APPROPRIATE DECISION MAKING AND PROBLEM
SOLVING TECHNIQUES -- The student will be able to:
05.01 Identify decision to be made and compare alternatives.
05.02 Apply decision making skills in the workplace.
05.03 Make decisions based on values and goals.
05.04 Evaluate the decision made.
05.05 Apply problem solving techniques in the workplace.
05.06 Diagnose problem, its urgency and causes.
05.07 Explore possible solutions to a problem & compare/contrast advantages.
05.08 Determine appropriate action; implement it and evaluate results.
05.09 Be sensitive to multicultural & nonsexist dimensions of problem solving.
06.0
DEMONSTRATE SPECIFIC LABORATORY SKILLS -- The student will be able
to:
06.01 Identify and quantify microorganisms and cells using manual & automated
systems.
06.02 Isolate, maintain & store pure cultures
06.03 Prepare seed inoculum.
06.04 Harvest cells & recover effluent products.
06.05 Transform cells.
06.06 Perform bioassays.
06.07 Decontaminate and/or dispose of equipment, glassware, biologicals.
06.08 Perform microbiology skills, including but not limited to , plating techniques,
isolating and characterizing cell lines, propagating plant and animal
cells, cryogenic techniques, and propagating and harvesting viruses.
06.09 Apply cell biology techniques, including but not limited to, transfection
techniques, monoclonal& polyclonal antibodies, &
hemagglutination/hemadsorption techniques.
06.10 Perform immunological techniques, including but not limited to, enzymelinked immunoabsorbent assays, probe and analyze DNA library, ,
transformation techniques, polymerase chain reaction, translation
assays, and construct recombinant vectors.
06.11 Perform genetic engineering and molecular biology techniques, including
isolate & analyze nucleic acid isolation, non-isotope techniques, disrupt
cells, protein gels, Western clotting, denature, renature proteins,
precipitate soluble proteins, concentrate(filter & dialyze) proteins,
quantitative proteins, and enzyme activity assays.
06.12 Perform additional specific skills, including but not limited to transcribing
DNA, electrophoresis on RNA,DNA & proteins, nucleic acid
hybridization, and autoradiography.
06.13 Separate ,isolate or characterize proteins, including but not limited to,
monitoring protein stability, quantitative analysis and distillation
techniques, titration techniques, employing dyes and indicators,
lypholization & organic chemistry techniques, and perform extractions.
06.14 Perform chemical assays including measuring turbidity, viscosity, &
density.
07.0
DEMONSTRATE QUALITY ASSURANCE/ CONTROL-- The student will be able
to:
07.01 Perform quality tests and document results.
07.02 Verify test standards and maintain QA records.
07.03 Archive samples and documents.
07.04 Inspect & verify integrity of product, procedure, and specimen.
07.05 Release final product and perform trend analysis.
07.06 Investigate complaints & take corrective action.
08.0
MAINTAIN FACILITY & EQUIPMENT -- The student will be able to:
08.01 Monitor/record the environmental condition of the facility (growth chamber,
laboratory, greenhouse, seed storage room, animal room or
manufacturing site).
08.02 Notify appropriate personnel if sampling indicates a problem.
08.03 Clean work area according to SOPs.
08.04 Label equipment.
08.05 Check calibration & perform systems diagnostics
08.05 Perform or schedule preventive maintenance.
08.06 Maintain equipment logs.
09.0
DEMONSTRATE PROPER CARE/USE OF TEST ANIMALS/PLANTS -- The
student will be able to:
09.01 Receive and transport animals.
09.02 Maintain separate in-process, quarantine and release areas
09.03 Feed, water and monitor animals.
09.04 Prepare animal food and prescription diets.
09.05 Clean housing and sterilize cages.
09.06 Monitor animal health and keep health records.
09.07 Follow USDA/IACUC guidelines for animal care.
09.08 Properly restrain and handle animals.
09.09 Collect and process specimens; collect data & document results.
09.10 Maintain plants for optimal growth.
09.11 Apply agrochemical safely.
09.12 Maintain and monitor insect populations.
09.13 Maintain plant growth media.
09.14 Inoculate plant and/or soil with biological materials.
09.15 Gather pollen and bundle pollinate.
09.16 Apply plant pests safely.
09.17 Collect data, perform bioassays, and document results of test plants.