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Enzyme Modeling Lab Lab Directions and Rubric This lab activity, revised from the Access Excellence1, will help you model how enzymes interact with their substrates under different environmental conditions. Overview: Compare the action of an enzyme on a substrate in normal vs. denatured conditions. Recall that denaturation occurs when the three dimensional shape of a protein is lost due to some external factor. You will not be submitting a formal lab report, but rather a table, graph and answers to thoughtprovoking discussion questions. The rubric for grading is below. Materials: ● a partner (a friend, sibling, or parent) ● 50 pennies placed tails side up for each trial ● stop watch ● masking tape Procedure: Trial I: Normal conditions Use fifty pennies placed tails-side up. Have your partner time you for ten seconds as you try to turn as many as you can from tails-side up to heads-side up. At the end of the first ten-second time period, record the number of pennies you turned in the Data Table and then DO NOT put them back in the pile of 50. You will continue turning the remaining pennies in ten second intervals. Each time, make sure to record your results in the table and DO NOT return the pennies to the pile. You are finished when you have turned all 50 pennies from tails-side up to heads-side up. Trial II: Abnormal conditions Enzyme Modeling Lab Page 1 of 3 Reset all of the pennies by turning all of them tails side up. Use the tape to bind the four fingers on your turning hand together. This represents partial denaturation of the enzyme. Repeat the procedure from Trial I by turning the pennies one by one in ten second intervals. You may need to add more ten-second periods if you still have pennies to turn. Make sure you record your data in the Trial II column of your data table after each 10 second period. Results: Record your data in a table similar to the one below. Time Periods (seconds) Trial I (# of pennies) Trial 2 (# of pennies) 0-10 10-20 20-30 30-40 40-50 50-60 60-70 70-80 Make a line graph (preferably showing two lines) that displays the number of pennies turned over per time period for both trials. ● Use a graphing program on your computer or one of the free online resources provided in the sidebar. ● Follow the guidelines in the Scientific Method module for constructing graphs. ● Use a different color for each trial (line) on the graph. Discussion Questions: Answer each in complete sentence(s). 1.What was the enzyme in this activity? 2.What was the substrate? 3.What would be an appropriate name for this enzyme? 4.In Trial I, what happens to the reaction rate (amount of product over time) as the supply of substrate runs out? Why? 5.Describe the difference between the graphs of reaction rates for Trial I and II. Explain why this is so. 6.Name at least one factor that could have caused the denaturation in Trial II (with real enzymes)? Enzyme Modeling Lab Page 2 of 3 7.This lab was supposed to model the action of an enzyme. Describe some sources of error in this lab. Submit your lab as one document if possible. Grading: The following criteria will be used to evaluate your lab: Criteria Points Possible Results Data Table (10) ● Properly set up per example ● Data included for both trials Graph (20) ● Digitally produced (not hand-drawn) ● Proper title ● Line graph(s) for both trials constructed (preferably) on same graph ● Axes placed properly and labeled with units ● Trial I/II colored or labeled clearly 30 Discussion Questions ● 10 points per question ● Answered in complete sentences 70 TOTAL 100 Enzyme Modeling Lab Page 3 of 3