Download Karyotype Analysis

Name_____________________________________
Date_______________
Period_______
Lab 6: Karyotype Analysis
Purpose: Analyze chromosomes in a karyotype to determine gender and genetic diseases.
Materials: Chromosomes of 4 different insects, scissors, glue
Procedure:
ANY INNAPROPRIATE BEHAVIOR WILL RESULT IN DISMISSAL FROM THE LAB.
Step 1: Cut out chromosomes for insect 1 and group homologous chromosomes together.
Step 2: Glue homologous chromosomes together on the data sheet from LARGEST to smallest.
Step 3: Glue the two sex chromosomes last (see example below).
Normal Male
Normal Female
Karyotypes for normal male and female.
Step 4: Compare your karyotype to the one above. If your insect’s karyotype is different, then there is a genetic disease.
Determine what genetic disorder they have by using the information below:
Disorder Name:
Size Reduction Disorder
Disorder
Disorder Name:
Clear Wing Disorder
Disorder Name:
Disorder Name:
Duplication Disorder
Unsegmented
Karyotype:
One chromosome missing
their homologous pair
Karyotype:
An extra chromosome
is present on pair 2
Karyotype:
An extra chromosome
is present on pair 1
Grading Rubric
1 – Below Basic
Hypothesis is missing;
Data table is incomplete;
Post Lab questions are
incorrect and not in
2 – Basic
Hypothesis is identified;
Data table is filled in;
Post Lab questions are
mostly incorrect or not in
3 – Proficient
Hypothesis is written;
Data table is complete;
Post Lab questions are
mostly correct in sentences.
Karyotype:
An chromosome
is missing on pair 2
4 – Advanced
Hypothesis is well written;
Data table is complete;
Post Lab questions are all
correct in well written
complete sentences.
sentences.
sentences.
DATA:
1. Question: Predict what type of chromosome disorder is more harmful: extra chromosome or missing chromosomes?
Develop a hypothesis (educated guess) that answers the experimental question. Write your hypothesis below.
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2. Data Table 1: Karyotypes
Insect 1
Insect 2
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Insect 4
Insect 5
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3. Data Table 2:
Gender (Male/Female)
Genetic Disorder Name
Insect 1:
__________________________________
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Insect 2:
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Insect 4:
__________________________________
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Insect 5:
__________________________________
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Post-Lab Questions: Answer questions in complete sentences on a separate sheet of paper.
1. How are chromosomes and karyotypes used to determine the gender of an individual? How would a male karyotype
look different then a female karyotype?
2. How can mutations cause genetic diseases? Why don’t mutations always result in an incorrect protein?
3. What is a transgenic organism? Why are Genetically Modified (GM) plants controversial?
4. What is the difference between reproductive cloning and therapeutic cloning?