Download CELL LINES

CELL LINES
When the primary culture is first
subcultured or passaged then it becomes
cell line.
TYPES OF CELL LINES
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FINITE CELL LINES :- Normal cell usually divide
only a limited number of times before loosing their
ability to proliferate which is a genetically determined
event known senescence these type of cell lines are
known as finite cell lines
CONTINUOUS CELL LINES :- When a finite cell
line undergoes transformation and acquires the
ability to divide indefinitely then it is known as
continuous cell lines.
TRANSFORMED CELL LINE:- It is defined as the
conversion of a normal healthy into a tumorous or a
cancerous cell
CHARACTERISTCS OF CONTINUOUS
CELL LINE
Smaller, more rounded ,less adherent with a
higher nucleus/cytoplasm ratio.
 Fast growth .
 Reduced anchorage dependence and grow more
in suspension condition.
 Ability to grow up to higher cell density.
 Different in phenotype from donor tissue.
 Stop expressing tissue specific gene.
 Homogeneous cell population.
 Serially propagated in culture showing an
increased growth.

EXAMPLES OF CONTINUOUS CELL LINE
CELL
LINE
MORPHOL
OGY
ORIGIN
SPECIES AGE
CHARACTERIS
TICS
A2780
Epithelial
Ovary
Human
Adult
Chemosensitive
with resistant
variants
Vero
Fibroblast
Kidney
Monkey
Adult
Viral substrate
and assay
IMR90
Fibroblast
Lung
Human
Embryo Susceptible to
nic
human viral
infection
WI-38
Fibroblast
Lung
Human
Embryo Susceptible to
nic
human viral
infection