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Supplementary Table 1. Identification of target genes of intermediary metabolism in barramundi. Assembled sequences were compared against known sequences in the database. For each barramundi gene, the corresponding Genbank BLAST hit, e-value probability of the BLAST hit being a random hit and % amino acid matches of each pairwise comparison are shown. Target Gene Glucose Metabolism Glucokinase (Hexokinase IV) Glucose-6-Phosphatase Gene Abbreviation Lc GK Lc G6Pase Fructose-1,6-Bisphosphatase Lc F1,6BPase Pyruvate Kinase Lc PK Phosphoenolpyruvate Carboxykinase, cytosolic Phosphoenolpyruvate Carboxykinase, mitochondrial Glucose-6-Phosphate Dehydrogenase Pyruvate Dehydrogenase Kinase Fatty Acid Metabolism Lc cPEPCK Lc mPEPCK Lc G6PDH Lc PDK Fatty Acid Synthase Lc FAS Stearoyl CoA Desaturase Lc SCD CarnitinePalmitoyltransferase Lc CPT1A ATP Citrate Lyase Lc acyl 3-hydroxybutyrate dehydrogenase Lc BDH2 hydroxyacyl-coenzyme A dehydrogenase Lc HOAD Amino Acid Metabolism Genbank Hit AAC33585 ADP37003 AFC36525 XP_003451042 ACV32405 ADD37845 XP_004070860 ACB72248 ACZ55139 AFP97552 CBN81352 XP_004078295 XP_003454250 XP_004065945 Description E-value % positive glucokinase [Sparus aurata] glucose-6-phosphatase [Lateolabrax japonicus] fructose-1,6-bisphosphatase [Lateolabrax japonicus] PREDICTED: pyruvate kinase isozymes R/L [Oreochromis niloticus] cytosolic phosphoenolpyruvate carboxykinase [Rachycentron canadum] mitochondrial phosphoenolpyruvate carboxykinase [Lateolabrax japonicus] PREDICTED: glucose-6-phosphate 1dehydrogenase-like, partial [Oryzias latipes] pyruvate dehydrogenase kinase 4 [Fundulus heteroclitus] 0 0 0 98 99 99 0 97 0 98 0 96 0 94 8.18E-17 92 fatty acid synthase, partial [Rachycentron canadum] stearoyl-CoA desaturase 1b [Sparus aurata] Carnitine O-palmitoyltransferase 1, liver isoform [Dicentrarchus labrax] PREDICTED: ATP-citrate synthase-like [Oryzias latipes] PREDICTED: 3-hydroxybutyrate dehydrogenase type 2-like [Oreochromis niloticus] PREDICTED: hydroxyacyl-coenzyme A dehydrogenase, mitochondrial-like isoform 1 [Oryzias latipes] 0 96 0 5.35E-52 93 88 2.57E-78 94 6.93E-94 98 0 95 Alanine Aminotransferase, cytosolic form Lc cALAT Aspartate Aminotransferase Lc ASAT Glutamate Dehydrogenase Lc GLUD1 CBN81871 XP_003454182 XP_003441805 Q6S9V7 Citrate Synthase Glycogen Metabolism Glycogen Synthase, liver isoform Glycogen Phosphorylase, liver isoform Nuclear Receptors Sterol Regulatory Element Binding Protein 1c Lc CS Lc gys2 Lc pygl Lc SREBP-1c XP_003456075 XP_003442910 XP_004558426 XP_003450741 Peroxisome ProliferatorActivated Receptor alpha Lc PPARa Liver X Receptor Farnesoid X Receptor Lc LXR Lc FXR AFK08803 BAN17451 Alanine aminotransferase 2-like [Dicentrarchus labrax] PREDICTED: aspartate aminotransferase, cytoplasmic-like [Oreochromis niloticus] PREDICTED: glutamate dehydrogenase, mitochondrial-like [Oreochromis niloticus] RecName: Full=Citrate synthase, mitochondrial; Flags: Precursor >gi|41058936|gb|AAR98860.1| mitochondrial citrate synthase precursor [Katsuwonus pelamis] 1.89E-127 96 1.93E-106 99 0 99 4.02E-92 100 PREDICTED: glycogen [starch] synthase, liver-like [Oreochromis niloticus] PREDICTED: glycogen phosphorylase, liver formlike isoform 1 [Oreochromis niloticus] 2.51E-142 98 0 99 PREDICTED: sterol regulatory element-binding protein 1-like [Maylandia zebra] PREDICTED: peroxisome proliferator-activated receptor alpha-like isoform 1 [Oreochromis niloticus] liver X receptor, partial [Sebastiscus marmoratus] farnesoid X receptor [Paralichthys olivaceus] 1.47E-21 95 3.93E-87 95 1.14E-101 1.23E-29 99 96