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ROBUST STUDY SUMMARY - Inherent Toxicity
Item
Yes
No
Reference:.Röderer G. 1990. Test-Bericht. Testung wassergefährdender Stoffe als Gruundlage für
Wasserqualitätsstandards. Frauenhofer-Institut für Umweltchemie und Ökotoxikologie. Schmallenberg,
Germany.
Test Substance: CAS # 56-23-5 and tetrachloromethane = Tetrachlorkohlenstoff
*Chemical composition of the substance ( including purity, by-products)
x
Persistence/stability of test substance in aquatic solution Flow-through test for
x
volatile substance, GC determination of actual concentrations.
Method
References
x
*OECD, EU, national, or other standard method? OECD-guidelines no 203 and 204
x
Justification of the method/protocol if not a standard method was used
*GLP (Good Laboratory Practice) ????? One of the most famous and best Institutes in
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Germany for scientific research
Test organisms (zebra fish (Brachydanio rerio))
Latin or both Latin and common names reported?
Life cycle age / stage of test organism
Sex
Length and weight of test organisms
2.0 cm
Number of test organisms per replicate 10 test organisms per test…
Food type / feeding periods (acclimation/during test) During acclimation TetraMin
food, not fed not fed during acute tests.
Test design / conditions
Test type – acute or chronic
acute:
Experiment type (laboratory or field) specified? Laboratory
System type (static, semi-static, flow-through)? Flow-through
Negative or positive controls (specify)?
Number of replicates (including controls) and concentrations 7
Exposure pathways (food, water, both)
Exposure duration 4 days
*Measured concentrations reported?
Exposure media conditions (temperature, pH, electrical conductivity, hardness, TOC,
DOC, DO, major cations and anions; other) (25 C, rest consistent with OECD
guidelines
Was pH within 6-9 range?
(8.15)
Was temperature within 5-28 °C range? (25°C)
Photoperiod and light intensity
light/ dark 14 / 10 hours
Stock and test solution preparation Detailed description of solution preparation and
flow-through ssystem
Use of emulgators / solubilizers (especially for poorly soluble / unstable substances)
no use…
Analytical monitoring intervals
Statistical methods used Litchfild and Wilcoxon and graphical
Results
Toxicity values (LC50, EC50, or IC50 ) 48h –and 96h- LC50 = 24.34mg/L
Other endpoints reported - BCF/BAF, LOEC/NOEC ( 14d-NOECo):
*Was toxicity value below the chemical’s water solubility?
WS = 793mg/L.
Other adverse effects (carcinogenicity, mutagenicity, etc. No)
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x
NA
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x
x
x
x
x
x
NA
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x
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x
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NA
NA
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x
Score: major items -
79.2%
EC Reliability code:
Reliability category (high, satisfactory, low):
Comments: excellent study, well reported….