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Table 5
Genes up-regulated in p8-/- liver after LPS
Fold induction
Protein synthesis, transport and modification
proteasome subunit MECL1 (#)
heat shock protein 86
proteasome subunit (lmp7)
ARGINYL-TRNA SYNTHETASE
p21
elongation factor-1 alpha (MS1)
alpha-D-mannosidase (Man2b1)
melanoma cell-derived intracisternal A-particle
calumenin
viral envelope like protein (G7e)
initiation factor 2-associated 67kDa
TI-225
phospholipase A2
apg-2
PA28 beta ribosomal protein L18 (rpL18)
subunit
26S proteasome ATPase (CIP21)
ancient ubiquitous 46 kDa protein AUP1 precursor (Aup1)
2-5 oligo A synthetase
co-chaperonin cofactor A
SKD1 protein
capping protein beta-subunit isoform 1
Sec61 protein complex gamma subunit
p162 protein
capping protein alpha 2 subunit
Golgi 4-transmembrane spanning transporter MTP
26S proteasome subunit 4 ATPase
translation initiation factor (Eif4g2)
vesicle trafficking protein sec22b
ribosomal protein L21
10.6
3.7
4.4
2.6
3.0
4.8
2.7
2.0
2.1
3.4
2.5
2.3
2.5
3.4
2.2
2.0
4.4
4.5
7.7
4.5
4.7
2.5
2.0
2.9
2.7
3.4
2.0
4.2
4.9
2.8
Signaling
SERINE/THREONINE PROTEIN KINASE
JAB
guanine nucleotide dissociation stimulator for a ras-related GTPase
interferon-inducible protein homologue
cytoplasmic phosphotyrosine phosphatase
intracellular calcium-binding protein (MRP8)
intracellular calcium-binding protein (MRP14)
GTPase IGTP
ras-GTPase-activating protein SH3-domain binding protein (G3BP)
suppressor of cytokine signalling-2 (SOCS-2)
suppressor of cytokine signalling-3 (SOCS-3)
retinally abundant regulator of G-protein signaling mRGS-r (RGS-r)
Wnt-7b
protein phosphatase 2A Balpha3 regulatory subunit
Indian hedgehog protein (Ihh)
Dvl-2
metallothionein II (MT-II)
OVARIAN GRANULOSA CELL 13.0 KD PROTEIN HGR74
manic fringe precursor
mothers-against-dpp-related-1
protein co-factor
interferon regulatory factor 7 (mirf7)
MYO-INOSITOL-1-PHOSPHATE SYNTHASE
GEG-154
seizure-related mRNA
T-cell specific protein
R-ras
calmodulin synthesis (CaM)
4.9
7.5
6.5
5.1
5.2
6.4
6.6
17.1
4.6
5.7
17.6
3.2
2.8
3.9
3.7
5.2
3.6
3.7
2.8
3.2
6.1
5.6
7.9
2.4
6.3
6.4
5.7
3.0
calcyclin
guanine nucleotide exchange factor delta subunit (JGR1)
cortactin
conserved helix-loop-helix ubiquitous kinase (CHUK)
G-protein-like LRG-47
IkB-beta
protein tyrosine phosphatase (mbptp1b)
TRAF-related protein (TRAFamn)
FK506 binding protein 51
Paneth cell enhanced expression PCEE
oxidative stress-induced protein
glucocorticoid-attenuated response gene 16 (GARG-16)
calcium-binding protein Cab45a
calcium-binding protein ALG-2 (ALG-257)
phosphoinositide 3-kinase regulatory subunit p85alpha
38 kDa Mov34 isologue
protein tyrosine phosphatase (PRL-1)
3.9
2.6
5.6
6.4
3.7
5.1
6.2
3.5
6.1
2.1
2.1
2.5
2.8
2.0
6.3
3.0
4.8
Secreted factors
presenilin-1
alpha-1 type IV collagen (Col4a-1)
serum amyloid A protein
plasminogen activator inhibitor (PAI-1)
monokine induced by gamma interferon (MIG)
interferon-gamma inducible protein (mag-1)
spi2 proteinase inhibitor (spi2/eb4)
IFN-gamma induced (Mg11)
B61
alpha-2 type IV collagen
major urinary protein I (MUP I)
major urinary protein IV (MUP IV)
mast cell protease-4
serum amyloid A protein (SAA5)
endothelial-monocyte activating polypeptide II
plasma glutathione peroxidase (MUSPGPX)
extracellular superoxide dismutase (SOD3)
endothelial monocyte-activating polypeptide I
metalloprotease/disintegrin/cysteine rich protein precursor (MDC9)
beta-galactoside binding lectin
cystatin B (Stfb)
C10-like chemokine
5.4
3.8
4.6
17.9
50.0
20.4
2.9
5.1
3.0
3.2
3.6
4.7
4.1
6.7
4.8
5.0
4.6
16.8
3.3
3.5
4.0
2.3
Cytoskeletal proteins
cytokeratin endo A
endo B cytokeratin
calpactin I light chain (p11)
alpha-tubulin isotype M-alpha-1
pancreatic beta-cell kinesin heavy chain
cytoplasmic beta-actin
SM22-ALPHA HOMOLOG
HGT keratin
cadherin-associated protein (CAP102/alpha catenin)
alkali myosin light chains
calpactin I heavy chain (p36)
tau microtubule binding protein
tctex-1
P-selectin
brain carcinoembryonic antigen (bCEA)
cadherin-11
8.9
6.2
2.0
2.6
2.0
2.4
2.2
3.9
2.1
3.0
3.7
3.5
3.3
3.1
2.6
9.9
Transcriptional factors and RNA processing
NUCLEAR RNA HELICASE
X box binding protein-1 (XBP-1)
CArG-binding factor-A
growth factor-induced protein (zif/268)
apurinic/apyrimidinic endonuclease (Apex)
gut-enriched Kruppel-like factor GKLF
4.4
7.9
2.3
3.0
6.3
4.1
transcription factor junB (junB)
origin recognition complex protein 2 homolog mORC2L
CACCC-box binding protein BKLF
Supt4h
putative purine nucleotide binding protein
ATP-dependent RNA helicase
transcriptional repressor (NAB1) NAB1
U1 snRNP-specific protein C
NNP-1 (NNP-1)
chromatin nonhistone high mobility group protein
RNA polymerase I 40kD subunit
gastrulation-brain-homeobox-2 (Gbx2)
GP106
CIRP
SCAFFOLD ATTACHMENT FACTOR
ELL homolog
nuclear LIM interactor (NLI)
homeobox protein Nkx2.6 (Nkx2.6)
heterogeneous nuclear ribonucleoprotein G (Hnrnpg)
DNA polymerase alpha associated subunit
MHLF
chromatin nonhistone high mobility group protein (HMG-I(Y)
RNA-binding protein
RNA helicase
HAM1
B/HLH/Z protein (Myn)
mATF4 (mTR67)
signal transducer and activator of transcription (Stat3)
11.6
3.5
4.4
2.6
15.8
4.4
3.3
4.5
5.7
6.0
3.3
2.2
3.0
2.8
3.9
2.0
7.8
7.2
2.4
2.8
4.6
8.5
5.5
2.1
3.9
2.2
5.0
3.3
Apoptotic-related proteins
BCL-W (Bcl-w)
EAT/MCL-1
FAF1
survival motor neuron (Smn)
inhibitor of apoptosis protein 1
NFIL3/E4BP4 transcription factor
ubi-d4
2.3
2.4
2.2
2.9
3.2
3.9
2.7
Membrane and transporters proteins
MAJOR VAULT PROTEIN
CD63/ME491
interleukin-1 receptor
interleukin-4 receptor (secreted form)
MHC class I Q8/9d cell surface antigen
Glvr-1
adipose differentiation related protein (ADFP)
chemokine rantes (Scya5)
Sca-2 precursor
mkf-1
Lisch7
inwardly-rectifying K+ channel protein (mb-IRK3)
leptin receptor (Ob-r)
semaphorin M-sema G
epimorphin
flotillin
polycystic kidney disease 2 (Pkd2)
aquaporin-8
vesicular GABA and glycine transporter
mast cell high affinity IgE receptor (Fc-epsilon-RI) gamma subunit
MHC class I Qa-Tla mRNA, (H2-d haplotype)
Tum-P91A antigen
MHC H-2K/t-w5-linked open reading frame
MHC class I H2-TL-T10-129 mRNA (b haplotype)
epidermal surface antigen (mesa)
tumor-associated membrane protein (TMP)
lymphotoxin-beta receptor
annexin V (Anx5)
2.7
6.0
6.5
5.2
2.4
3.8
4.8
11.2
6.4
2.6
6.5
3.8
3.5
2.2
9.4
2.5
3.6
7.4
4.1
2.0
4.5
5.3
3.2
7.0
3.2
3.0
2.1
3.6
interleukin 17 receptor
aminopeptidase
scavenger receptor class B type I (mSR-BI)
membrane-type matrix metalloproteinase 1
LDL RECEPTOR-RELATED PROTEIN
photolyase/blue-light receptor homolog
3.8
2.9
2.0
2.4
2.1
4.7
Cytosolic enzymes and detoxification factors
UDP-galactose transporter related isozyme 1
transglutaminase (TGase)
serine palmitoyltransferase LCB2 subunit
liver arginase
preprodipeptidyl peptidase I
squalene epoxidase,
spermidine/spermine N1-acetyltransferase (SSAT)
L-SERINE DEHYDRATASE
PLACENTAL RIBONUCLEASE INHIBITOR
stearoyl-CoA desaturase
LACTATE DEHYDROGENASE
X chromosome-linked phosphoglycerate kinase (pgk-1)
GLUTAREDOXIN
2.5
16.3
2.1
2.0
5.8
3.0
4.1
2.2
2.7
2.9
3.6
2.6
3.4
Growth-related proteins
Gadd45
p21 (Waf1)
Cdk-inhibitor p57KIP2 (KIP2)
retinoblastoma-binding protein (mRbAp48)
p160 myb-binding protein (P160)
spindlin (Spin)
Nmi
Neuropilin
cyclin-dependent kinase homologue (p130PITSL)
nerve growth factor beta (beta-NGF)
24.6 kda protein
3.4
3.3
3.3
4.0
3.7
6.2
2.4
2.4
2.1
3.2
2.1
# Underlined genes are also up-regulated in liver from p8+/+ mice
Table 6
Genes down-regulated in p8-/- liver after LPS
Fold repression
Protein synthesis, transport and modification
mitochondrial stress-70 protein (PBP74/CSA)
7.3
Signaling
antioxidant protein 2 (AOP2) (§)
Selenium-binding liver protein
inhibitor protein of cAMP-dependent protein kinase
brain-specific kinase (Bsk)
GTP-BINDING PROTEIN
PROTEIN KINASE C INHIBITOR 1
ras-GTPase-activating protein SH3-domain binding protein
SNF1-RELATED KINASE
4.1
3.7
2.7
2.2
2.3
2.1
2.9
3.9
Secreted factors
D-dopachrome tautomerase (Ddt)
serine protease hepsin
monoglyceride lipase
heparin cofactor II precursor (HCII)
cardiotrophin-1
bone morphogenetic protein 8B (Bmp8b)
mast cell protease-2 (MMCP-2)
BMP-4
prealbumin
sialoprotein II
2.8
2.3
7.3
6.3
4.1
3.3
2.4
2.8
2.3
2.4
Cytoskeletal proteins
fibroglycan (syndecan-2)
pericentrin
erythroid alpha-spectrin (Spna1)
3.4
3.1
3.5
Transcriptional factors and RNA processing
BAP-135 homolog (Diws1t)
Parathymosin
hypoxia-inducible factor 1 alpha (Hif1a)
friend of GATA-1 (FOG)
transcription factor S-II-related protein
8-oxo-dGTPase
HISTONE H2A.Z
musashi-1 gene for RNA-binding protein
homeobox protein Nkx3.1
7.0
2.0
2.5
4.4
9.0
3.4
2.9
3.4
3.0
Apoptotic-related proteins
IAP homolog A (MIHA)
2.0
Membrane and transporters proteins
multidrug resistance protein
microsomal triglyceride transfer protein
asialoglycoprotein receptor (MHL-1)
retinol binding protein (RBP)
NPC1 (Npc1)
PE31/TALLA
importin alpha Q2
orphan nuclear hormone receptor isoform mCAR2
ryudocan core protein
GABA-A receptor gamma-2 subunit
6.7
3.4
2.6
2.3
2.3
3.7
3.1
12.3
9.5
2.3
Cytosolic enzymes and detoxification factors
carnitine acetyltransferase
TRANS-1,2-DIHYDROBENZENE-1,2-DIOL DEHYDROGENASE
amine N-sulfotransferase (SULT-N)
sulfotransferase-related protein (SULT-X2)
3.2
2.5
4.9
9.6
alanine:glyoxylate aminotransferase (Agxt1)
very-long-chain acyl-CoA synthetase (VLCS)
4-hydroxyphenylpyruvate dioxygenase
estradiol 17-beta-dehydrogenase (A-specific)
catalase
hydroxysteroid sulfotransferase (mSTa2)
ornithine transcarbamylase
adh-1 gene encoding alcohol dehydrogenase (ADH)
malate dehydrogenase
aldehyde dehydrogenase II
fumarylacetoacetate hydrolase (Fah)
alcohol dehydrogenase-B2 (Adh-2)
thioether S-methyltransferase
glucose-6-phosphatase
sorbitol dehydrogenase precursor
homogentisate 1,2-dioxygenase (HGO)
flavin-containing monooxygenase 3
flavin-containing monooxygenase 1 (FMO1)
flavin-containing monooxygenase 5 (FMO5)
GLYCINE DEHYDROGENASE
branched chain alpha ketoacid decarboxylase E1a subunit
cytochrome P-450
adrenodoxin
cytochrome P450 monooxygenase (CYP2J5)
lysosomal sialic acid O-acetylesterase
liver glutathione transferase theta class type 2
testosterone 16-alpha-hydroxylase
ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT
2,4-DIENOYL-COA REDUCTASE
FATTY ACID-BINDING PROTEIN
3-hydroxyacyl CoA dehydrogenase
cytosolic epoxide hydrolase
3-beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase
argininosuccinate synthetase (Ass)
PROTEIN DISULFIDE ISOMERASE-RELATED PROTEIN
selenophosphate synthetase 2
NADH-UBIQUINONE OXIDOREDUCTASE B9 SUBUNIT
NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT
ATP SYNTHASE E CHAIN
sperm mitochondrial capsule seleno-protein (MCS)
NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase
21.0
4.0
4.9
7.2
3.5
3.7
2.1
5.0
2.5
2.2
2.8
2.1
29.9
3.6
8.0
7.7
7.5
7.1
2.7
2.0
3.5
3.5
3.2
3.2
2.8
5.0
2.2
2.9
2.8
5.4
4.6
2.5
3.2
2.7
2.2
2.0
2.2
2.7
2.1
2.0
4.0
Growth-related proteins
retinoblastoma-related protein Rb2/p130
SCID complementing gene 2
p13MTCP1 (MTCP1)
3.3
2.2
2.6
§ Underlined genes are also down-regulated in liver from p8+/+ mice