Download Tutorial_3_2009

UNIVERSITY OF TECHNOLOGY, JAMAICA
FACULTY OF SCIENCE AND SPORTS
SEMESTER 1: Tutorial 3
DATE: September 18, 2009
GROUP: BSc. Science and Education, Year 4
MODULE: Molecular Biology (BIO1004/2001)
1.
DNA replication is semi-conservative, what does this mean? If DNA replication were
conservative, where would the DNA band in the centrifuge tube be after one round of DNA
replication in the Messelson Stahl experiment?
2.
Diagram the process of semi conservative replication of DNA by drawing the replication
fork and indicating important enzymes, proteins, and other components involved in this
process. Provide a one sentence description of the function of each component
3.
DNA replication is bi-directional. What would the replication bubble look like if replication was
unidirectional? What is the start site for DNA replication?
4.
Why do some mutations affect protein structure and function that can result in disease
whereas other mutations have no significant effects on protein structure and function?
5.
The following short sentence represents a gene:
WET WET WET ARE NOT ALL BAD
What types of mutations are the following?
a.
b.
c.
d.
e.
f.
g.
WEE
WET
WEW
WET
WET
WET
WET
WET
WET
ETW
WET
WET
WET
WET
WET
WET
ETA
WET
ARE
WEG
WET
ARE
ARE
REN
ART
NOT
OOD
WET
NOT
COT
OTA
TEN
ALL
MOR
WET
ALL
ALL
LLB
TAL
BAD
NIN
WET
BAD
BAD
AD
LBA D
GTA REN OTA LLB AD
ARE NOT ALL BAD
6. What type of mutation is AGAGCCGAGGA --> AGAGCGAGGA?
7. Outline some differences between replication in prokaryotes and eukaryotes.
A. deletion B. inversion C. frameshift
D. insertion E. point mutation
8. A DNA copy of a sequence of DNA is synthesized during
A. replication. B. transcription. C. translation.
D. transformation. E. none of these choices are true
9. DNA replication along the 3' --> 5' template strand
A. is continuous. B. is discontinuous.
C. cannot occur. D. forms a lagging strand.
E. must loop the DNA
References
http://www.colorado.edu/MCDB/MCDB1150/Fall06/ExamFourPracticeQuestionsFall06.doc
http://www.csuchico.edu/~jbell/Biol207/final.html