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JOHNS HOPKINS UNIVERSITY
Department of Biology
Instructor Name:
Course Title: Biochemistry Laboratory
Robert D. Horner, Ph.D.
Instructor Contact Information
Course Number: 020.315.21
179 UTL, Department of Biology
Distribution: N
410-516-8067 or -7281
[email protected]
Instructor Office Hours/Location: Tues. & Thurs. 1-2
Credits: 2
p.m., 179 UTL .
Lab Lecture: M, W, F noon -1:00 p.m. in Mudd 100
Dates: July 3 – August 4, 2017
Lab Section: M, W, F 1:30 -4:30 p.m. in 174 UTL .
COURSE DESCRIPTION
This course will reinforce the topics presented in Biochemistry 020.305 through laboratory exercises which use quantitative
measurement to study cellular components. Topics include pH, proteins, carbohydrates, lipids, nucleic acids, and enzymes.
COURSE LEARNING OBJECTIVES
Students should obtain a good working knowledge and basic understanding of the following areas:
1
Buffers, pH and weak acids in proteins and nucleic acids
2
Use of spectrophotometry to measure biomolecules and biochemical processes
2
Enzyme catalysis, purification, quantitation and electrophoretic analysis
3
Measurement of binding constants and free energy of protein unfolding
4
Chemical characterization of carbohydrates and lipids
5
DNA isolation and restriction enzyme analysis
6
Polymerase chain reaction
REQUIRED MATERIALS
TEXTBOOKS: 1. Manual online at course website
2. Berg, J.M., Tymoczko, J.L., & Stryer, L., Biochemistry, 7th Ed.
SUPPLIES: Experimental Research Notebook with carbonless
duplicating pages.
COURSE WEBSITE: on Blackboard
EVALUATION AND GRADING
Grades will be based on the following assessments:
ASSESSMENT TYPE
Several class assessments during lecture
Eleven lab reports
Two midterm exams
Eleven quizzes, one at the beginning of each lab
Lab practical examination
https://blackboard.jhu.edu/webapps/portal/frameset.jsp
PERCENT OF FINAL GRADE
2%
19%
34%
19%
26%
ATTENDANCE POLICY
Students are required to attend all lab and lecture sessions. Students are required to inform the instructor before class or lab if
they must miss the session.
JOHNS HOPKINS POLICIES AND SUPPORT SERVICES
This course is governed by the policies set forth in The Johns Hopkins University Undergraduate Student Handbook, which
contains information on a wide variety of topics, such as support services, and policies relating to student rights and
responsibilities. This course is governed by the policies set forth in this document.
Some JHU student support services you may find useful include:
SUPPORT SERVICE
LOCATION
PHONE NUMBER / WEBSITE
Library E-Reserves
http://www.library.jhu.edu/services/forms/reserves.html
[email protected]
The Learning Den
provides tutoring for all
registered students in Arts and
Sciences and Engineering. No
fee and no limit to the number of
sessions a student may attend.
Summer & Intersession
Programs
Homewood Campus in Gilman Hall and nearby
buildings
[email protected]
Ms. Anne Benner
Garland Hall, Suite 300
410-516-8216
3505 N. Charles Street, Suite 101
410-516-4548
http://www.jhu.edu/intersession
CLASSROOM ACCOMODATIONS FOR STUDENTS WITH DISABILITIES
If you are a student with a documented disability who requires an academic adjustment, auxiliary aid or other similar
accommodations, please contact The Office of Student Disability Services at [email protected],
call 410-516-4720 or visit 385 Garland Hall.
STATEMENT OF DIVERSITY AND INCLUSION
Johns Hopkins University is a community committed to sharing values of diversity and inclusion in order to achieve and sustain
excellence. We believe excellence is best promoted by being a diverse group of students, faculty and staff who are committed to
creating a climate of mutual respect that is supportive of one another’s success. Through its curricula and clinical experiences, we
purposefully support the University’s goal of diversity, and in particular, work toward an ultimate outcome of best serving the
needs of students. Faculty and candidates are expected to demonstrate an understanding of diversity as it relates to planning,
instruction, management, and assessment.
A WORD ON ETHICS
Ethics:
Because the strength of the university depends on academic and personal integrity, the Course Instructor of this course
expects students to be follow the code of ethics established by the undergraduate students of the Johns Hopkins University in
1975. In this course, you must be honest and truthful. Ethical violations include cheating on exams, plagiarism, reuse of
assignments, improper use of the Internet and electronic devices, unauthorized collaboration, alteration of graded assignments,
forgery and falsification, lying, facilitating academic dishonesty, and unfair competition. Ignorance of these rules is not an
excuse. The university will punish cheaters with failure on an assignment, failure in a course, permanent transcript notation,
suspension, and/or expulsion. Offenses may be reported to medical, law or other professional or graduate schools when a cheater
applies.
In addition, the specific ethics guidelines for this course are:
Quizzes and the final practical exam are closed book.
The only calculators allowed during test periods are TI-30 series calculators. Calculators may not be shared between students
during quizzes or the practical exam.
Lab reports must be the product of a student’s own effort.
Report any violations you witness to the instructor. You may consult the associate dean of students and/or the chairman of the
Ethics Board beforehand. See the guide on AAcademic Ethics for Undergraduates@ and the Ethics Board web site
(http://ethics.jhu.edu) for more information.
HELPFUL ADVICE
For suggestions on lecture note-taking, please Google “Cornell note-taking”.
For a productive text reading method, please Google “SQ3R”.
This course covers in four weeks what is normally covered in the 13 weeks of a semester. If you do not have the prerequisites of
this course, you will be at risk for failure.
A course website has been constructed on Blackboard. Abbreviated lectures will be posted. If you are registered in the course,
your JHED ID and password will give you access. The URL is https://blackboard.jhu.edu/webapps/portal/frameset.jsp
The instructor and teaching assistant will monitor the discussion board, but since the course meets three times a week, questions
are best asked in lecture and lab so everyone will hear and benefit.
COURSE SCHEDULE
Day
Lab
Lecture
7/3
1. Set up
Intro., pH, Buffers and Amino Acids
7/5
2. pH, Buffers, Amino Acids
Spectrophotometry
7/7
3. Spectrophotometry
Protein Folding
7/10
4. Protein Folding
Protein-Ligand Binding
7/12
5. Protein-Ligand Binding
Enzyme Catalysis
7/14
6. Enzyme Catalysis
Carbohydrate Chem.
7/17
7. Carbohydrate Chemistry
Protein Purif. & Lysozyme Enzyme Mech
7/19
8. Protein Purification
Midterm Exam I (labs 1-5)
7/21
9. Lysozyme Enzyme Mechanism
Prot. Electrophor.
7/24
10. Protein Electrophoresis
DNA Isolation & PCR
7/26
11. DNA Isolation & PCR
Restriction Enz.
7/28
12. Restriction Enzymes
Midterm exam II (labs 6-9)
7/31
13. Practical Exam
Review