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LABORATORY REPORT
Activity 13: Acid-Base Balance
Name:
Instructor:
Date:
PREDICTIONS
1. Compared to normal blood pH, arterial blood pH levels in patients with acidosis are
2. Compared to normal blood pH, arterial blood pH levels in patients with alkalosis are
3. Arterial blood pCO2 levels in patients with respiratory acidosis are
4. Arterial blood HCO3– levels in patients with metabolic acidosis are
5. Arterial blood pCO2 levels in patients with respiratory alkalosis are
6. Arterial blood HCO3– levels in patients with metabolic alkalosis are
MATERIALS AND METHODS
1. Dependent Variable
2. Independent Variable
3. Controlled Variables
4. Explain why HCO3– levels can be calculated from pH and pCO2 levels.
5. In this experiment, HCO3– concentrations are calculated using pH and pCO2 concentrations. Refer to
the following chemical reaction to describe how changes in CO2 and H+ concentrations affect HCO3–
concentration.
CO2 + H2O ⇌ H2CO3 ⇌ H+ + HCO3–
RESULTS
See Table 2: Acid-Base Imbalance Signs and Compensation
1. The normal range respiratory rate is 12 — 18 breaths/min). For each patient, type in their respiratory
rate and indicate if it is lower, within, or higher than the normal range.
a.
Patients with respiratory acidosis
b.
Patient with respiratory alkalosis
c.
Patient with metabolic acidosis
d.
Patient with metabolic alkalosis
2. The normal range for blood pH is 7.35 — 7.45 . For each patient, type in their blood pH and indicate if
it is lower, within, or higher than the normal range.
a.
Patients with respiratory acidosis
b.
Patient with respiratory alkalosis
c.
Patient with metabolic acidosis
d.
Patient with metabolic alkalosis
3. The normal range for blood pCO2 is 35 — 45 mmHg. For each patient, type in their blood pCO2 levels
and indicate if it is lower, within, or higher than the normal range.
a.
Patients with respiratory acidosis
b.
Patient with respiratory alkalosis
c.
Patient with metabolic acidosis
d.
Patient with metabolic alkalosis
4. The normal range for blood HCO3– is 22 — 26 mEq/L . For each patient, type in their blood HCO3– and
indicate if it is lower, within, or higher than the normal range.
a.
Patients with respiratory acidosis
b.
Patient with respiratory alkalosis
c.
Patient with metabolic acidosis
d.
Patient with metabolic alkalosis
DISCUSSION
1. Is compensation occurring in the patient with respiratory acidosis? State which system (respiratory or
renal) is involved in compensation and what compound is being removed or added to achieve
compensation. Describe how this substance is being removed or added and how removing or adding this
compound returns pH toward normal.
2. Is compensation occurring in the patient with respiratory alkalosis? State which system (respiratory or
renal) is involved in compensation and what compound is being removed or added to achieve
compensation. Describe how this substance is being removed or added and how removing or adding this
compound returns pH toward normal.
3. Is compensation occurring in the patient with metabolic acidosis? State which system (respiratory or
renal) is involved in compensation and what compound is being removed or added to achieve
compensation. Describe how this substance is being removed or added and how removing or adding this
compound returns pH toward normal.
4. Is compensation occurring in the patient with metabolic alkalosis? State which system (respiratory or
renal) is involved in compensation and what compound is being removed or added to achieve
compensation. Describe how this substance is being removed or added and how removing or adding this
compound returns pH toward normal.
5. Restate your predictions that were correct and give the data from your experiment that supports
them. Restate your predictions that were not correct and correct them, giving the data from your
experiment that supports the corrections.
APPLICATION
1. Patients with COPD (Chronic Obstructive Pulmonary Disease) may develop respiratory acidosis.
Explain how COPD causes respiratory acidosis and why breathing rate is elevated in COPD patients with
respiratory acidosis.
2. You can only hold your breath for a limited period of time before you involuntarily begin breathing
again. Describe feedback mechanisms that result in involuntary breathing.
3. Anxiety can cause hyperventilation. Explain how anxiety can cause hyperventilation and how
hyperventilation causes respiratory alkalosis.
4. Patients with uncontrolled diabetes can develop metabolic acidosis. Explain how uncontrolled
diabetes causes metabolic acidosis.