Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
How does DNA determine the traits of an Ice Cream Sundae? Purpose is to examine the DNA sequence of an ice cream sundae Ice Cream Sundaes only have one chromosome with 10 genes on it. Procedure: You must analyze the genes of its DNA to determine what traits the sundae has and then create a model of your ice cream sundae. These genes must be assembled in order 1. Transcribe your DNA sequence into a mRNA sequence (do not forget to break your sequence into codon segments). 2. Use a genetic code chart to translate your mRNA codons into amino acids. 3. Use the amino acid sequence to determine what trait (phenotype) each gene will express for your ice cream sundae. 4. After completing step 3 – ask your teacher to verify the accuracy of your ice cream sundae gene expression. For simplicity, the gene sequences are much smaller than –real- gene sequences found in living organisms. Each gene has two versions that result in a different trait being expressed on a sundae. ©copyright 2014-all rights reserved www.cpalms.org Genes Amino Acid Sequence Gene 1 – ice cream type MET-VAL- SER- LEU-STOP MET-VAL- SER- LYS-STOP Gene 2 – ice cream type Gene 3 – MET-TYR – PRO- GLU- GLU- LYSSTOP MET-VAL- PRO- THR- GLN- LYSSTOP MET-LEU- LEU- LEU- PRO-STOP MET-LEU- LEU- SER- ALA-STOP Gene 4 – MET-ALA- VAL- VAL-STOP MET-VAL- ALA- ALA-STOP Gene 5 – MET-HIS – ILE-STOP METH-HIS- HIS-STOP Gene 6 – MET-SER- PRO- VAL-STOP MET-VAL- PHE- TYR-STOP Gene 7 – Gene 8 – MET-ASN- ILE- LEU- LEU- PROTHR-STOP MET-ASN- ILE- PRO- PRO- PROTHR-STOP MET-VAL- ASN- ASN- ALA-STOP MET-ASN- ASN- ASN- ALA-STOP Gene 9 – MET-PHE- SER- GLY-STOP MET-PHE- PHE- GLY-STOP Gene 10 – MET-ARG- TYR- CYS- LYS-STOP MET-ARG- ARG- ASN- THR-STOP ©copyright 2014-all rights reserved www.cpalms.org Description Chocolate Vanilla Fudge Swirl Neopolitan M&M’s Chocolate Chips Redi-Whip Marshmallows Twix Kit Kat Chocolate Syrup Caramel Syrup Oreos Jelly Beans Snickers Bites Reeses Pieces Round Sprinkles Shaped (not round) Sprinkles Cherry Strawberry (Level 4)DNA with 10 gene sequences each with a start and stop codon including “junk” Sundae 1 TACCAGTCGTTTATTTAAAGCCATATTTACATGGGGGTTGTCTTTACTTA AAGCCATATTTACGAGAATTCACGCATCTAAAGCCATATTTACCGACAAC ACATTTAAAGCCATATTTACGTAGTAATCTAAAGCCATATTTACCAAAA AATGATTTAAAGCCATATTTACTAATAGAATGACGGGTGGATTTAAAGC CATATTTACTTATTGTTACGGATCTAAAGCCATATTTACAAAAGACCGAC TTAAAGCCATATTTACGCGATGACATTTATTTAAAGCCATATT Sundae 2 TACCATAGATTTATTTAAAGCCATATTTACCAAGGATGAGTTTTCACTT AAAGCCATATTTACGAAGAGGAGGGGATCTAAAGCCATATTTACCAACG CCGAATTTAAAGCCATATTTACGTAGTGATCTAAAGCCATATTTACCATA AAATAACTTAAAGCCATATTTACTTATAAGAAGACGGGTGTACTTAAAG CCATATTTACTTATTATTACGTATCTAAAGCCATATTTACAAGAGCCCGA TTTAAAGCCATATTTACGCGGCGTTATGAATTTAAAGCCATATT ©copyright 2014-all rights reserved www.cpalms.org Sundae 3 TACCAGTCGTTTATTTAAAGCCATATTTACATGGGGGTTGTCTTTACTTA AAGCCATATTTACGAGAATTCAGCGATCTAAAGCCATATTTACCAACGCC GAACTTAAAGCCATATTTACGAGTAAATCTAAAGCCATATTTACAGAGG GCATATTTAAAGCCATATTTACTTATAAGAGGAGGGGTGGATTTAAAGC CATATTTACCAATTATTACGTATCTAAAGCCATATTTACAAGAAACCCAC TTAAAGCCATATTTACGCAGCCTTGTGTATTTAAAGCCATATT Sundae 4 TACCATAGGGAGATTTAAAGCCATATTTACATGGGGGTTGTCTTTATTT AAAGCCATATTTACAATAACGACGGGATCTAAAGCCATATTTACCACCGT CGAACTTAAAGCCATATTTACGTATAAATTTAAAGCCATATTTACAGAG GGCATACTTAAAGCCATATTTACTTGTAAGGAGGGGGGTGTATTTAAAG CCATATTTACCATTTGTTACGGATTTAAAGCCATATTTACAAAAGACCGA CTTAAAGCCATATT TACTCTTCCTTATGCATT TAAAGCCATATT ©copyright 2014-all rights reserved www.cpalms.org (Level 3)DNA with 10 gene sequences each with a start and stop codon Sundae 1 TACCAGTCGTTTATTTACATGGGGGTTGTCTTTACTTACGAGAATTCACG CATCTACCGACAACACATTTACGTAGTAATCTACCAAAAAATGATTTACT AATAGAATGACGGGTGGATTTACTTATTGTTACGGATCTACAAAAGACC GACTTACGCGATGACATTTATT Sundae 2 TACCATAGATTTATTTACCAAGGATGAGTTTTCACTTACGAAGAGGAGG GGATCTACCAACGCCGAATTTACGTAGTGATCTACCATAAAATAACTTAC TTATAAGAAGACGGGTGTACTTACTTATTATTACGTATCTACAAGAGCC CGATTTACGCGGCGTTATGAATT Sundae 3 TACCAGTCGTTTATTTACATGGGGGTTGTCTTTACTTACGAGAATTCAGC GATCTACCAACGCCGAACTTACGAGTAAATCTACAGAGGGCATATTTACT TATAAGAGGAGGGGTGGATTTACCAATTATTACGTATCTACAAGAAACC CACTTACGCAGCCTTGTGTATT Sundae 4 TACCATAGGGAGATTTACATGGGGGTTGTCTTTATTTACAATAACGACG GGATCTACCACCGTCGAACTTACGTATAAATTTACAGAGGGCATACTTAC TTGTAAGGAGGGGGGTGTATTTACCATTTGTTACGGATTTACAAAAGAC CGACTTACTCTTCCTTATGCATT ©copyright 2014-all rights reserved www.cpalms.org (Level 2)DNA with 10 gene sequences each with a start and stop codon Sundae 1 1 2 3 4 5 6 7 8 9 10 TACCAGTCGTTTATT TACATGGGGGTTGTCTTTACT TACGAGAATTCACGCATC TACCGACAACACATT TACGTAGTAATC TACCAAAAAATGATT TACTAATAGAATGACGGGTGGATT TACTTATTGTTACGGATC TACAAAAGACCGACT TACGCGATGACATTTATT Sundae 2 1 2 3 4 5 6 7 8 9 10 TACCATAGATTTATT TACCAAGGATGAGTTTTCACT TACGAAGAGGAGGGGATC TACCAACGCCGAATT TACGTAGTGATC TACCATAAAATAACT TACTTATAAGAAGACGGGTGTACT TACTTATTATTACGTATC TACAAGAGCCCGATT TACGCGGCGTTATGAATT ©copyright 2014-all rights reserved www.cpalms.org Sundae 3 1 2 3 4 5 6 7 8 9 10 TACCAGTCGTTTATT TACATGGGGGTTGTCTTTACT TACGAGAATTCAGCGATC TACCAACGCCGAACT TACGAGTAAATC TACAGAGGGCATATT TACTTATAAGAGGAGGGGTGGATT TACCAATTATTACGTATC TACAAGAAACCCACT TACGCAGCCTTGTGTATT Sundae 4 1 2 3 4 5 6 7 8 9 10 TACCATAGGGAGATT TACATGGGGGTTGTCTTTATT TACAATAACGACGGGATC TACCACCGTCGAACT TACGTATAAATT TACAGAGGGCATACT TACTTGTAAGGAGGGGGGTGTATT TACCATTTGTTACGGATT TACAAAAGACCGACT TACTCTTCCTTATGCATT ©copyright 2014-all rights reserved www.cpalms.org (Level 1) Gene sequences in mRNA code – codons separated Sundae 1 AUG GUC AGC AAA UAA\ AUG UAC CCC CAA CAG AAA UGA\ MET CUC UUA AGU GCG UAG\ MET GCU GUU GUG UAA\ MET CAU CAU UAG\ MET GUU UUU UAC UAA\ MET AUU AUC UUA CUG CCC ACC UAA\ MET AAU AAC AAU GCC UAG\ MET UUU UCU GGC UGA\ MET CGC UAC UGU AAA UAA Sundae 2 AUG GUA UCU AAA UAA\ AUG GUU CCU ACU CAA AAG UGA\ AUG CUU CUC CUC CCC UAG\ AUG GUU GCG GCU UAA\ AUG CAU CAC UAG\ AUG GUA UUU UAU UGA\ AUG AAU AUU CUU CUG CCC ACA UGA\ AUG AAU AAU AAU GCA UAG\ AUG UUC UCG GGC UAA\ AUG CGC CGC AAU ACU UAA ©copyright 2014-all rights reserved www.cpalms.org Sundae 3 AUG GUC AGC AAA UAA\ AUG UAC CCC CAA CAG AAA UGA\ AUG CUC UUA AGU CGC UAG\ AUG GUU GCG GCU UGA\ AUG CAC AUU UAG\ AUG UCU CCC GUA UAA\ AUG AAU AUU CUC CUC CCC ACC UAA\ AUG GUU AAU AAU GCA UAG\ AUG UUC UUU GGG UGA\ AUG CGU CGG AAC ACA UAA Sundae 4 AUG GUA UCC CUC UAA\ AUG UAC CCC CAA CAG AAA UAA\ AUG UUA UUG CUG CCC UAG\ AUG GUG GCA GCU UGA\ AUG CAU AUU UAA\ AUG UCU CCC GUA UGA\ AUG AAC AUU CCU CCC CCC ACA UAA\ AUG GUA AAC AAU GCC UAA\ AUG UUU UCU GGC UGA\ AUG AGA AGG AAU ACG UAA ©copyright 2014-all rights reserved www.cpalms.org Teacher Key Sundae 1 1 AUG GUC AGC AAA UAA\Met-Val-Thr-Lys-Stop 2 AUG UAC CCC GAA GAG AAA UGA\Met-Tyr-Pro-Glu-Glu-Lys-Stop 3 AUG CUC UUA AGU GCG UAG\ Met-Leu-Leu-Ser-Ala-Stop 4 AUG GCU GUU GUG UAA\ Met-Ala-Val-Val-Stop 5 AUG CAU CAU UAG\ Met-His-His-Stop 6 AUG GUU UUU UAC UAA\ Met-Val-Phe-Tyr-Stop 7 AUG GAU AUC UUA CUG CCC ACC UAA\ Met-Asp-Ile-Leu-Leu-Pro-Thr-Stop 8 AUG AAU AAC AAU GCC UAG\ Met-Asn-Asn-Asn-Ala-Stop 9 AUG UUU UCU GGC UGA\ Met-Phe-Ser-Gly-Stop 10 AUG CGC UAC UGU AAA UAA\ Met-Arg-Tyr-Cys-Lys-Stop =Vanilla =Fudge Swirl =Chocolate Chips =Redi-Whip =Kit Kat =Caramel Syrup =Oreo =Reeses Pieces =Round Sprinkles =Cherry Sundae 2 1 AUG GUA UCU AAA UAA\ Met-Val-Ser-Lys-Stop 2 AUG GUU CCU ACU CAA AAG UGA\ Met-Val-Pro-Thr-Gln-Lys-Stop 3 AUG CUU CUC CUC CCC UAG\ Met-Leu-Leu-Leu-Pro-Stop 4 AUG GUU GCG GCU UAA\ Met-Val-Ala-Ala-Stop 5 AUG CAU CAC UAG\ Met-His-His-Stop 6 AUG GUA UUU UAU UAG\ Met-Val-Phe-Tyr-Stop 7 AUG AAU AUU CUU CUG CCC ACA UGA\ Met-Asn-Ile-Leu-Leu-Pro-Thr-Stop 8 AUG AAU AAU AAU GCA UAG\ Met-Asn-Asn-Asn-Ala-Stop 9 AUG UUC UCG GGC UAA\ Met-Phe-Ser-Gly-Stop 10 AUG CGC CGC AAU ACU UAA\Met-Arg-Arg-Asn-Thr-Stop =Vanilla =Neopolitan =M&M’s =Marshmallows =Kit Kat =Caramel Syrup =Oreos =Reeses Pieces =Round Sprinkles =Strawberry Sundae 3 1 AUG GUC AGC AAA UAA\ Met-Val-Ser-Lys-Stop 2 AUG UAC CCC GAA GAG AAA UGA\ Met-Tyr-Pro-Glu-Glu-Lys-Stop 3 AUG CUC UUA AGU CGC UAG\ Met-Leu-Leu-Ser-Ala-Stop 4 AUG GUU GCG GCU UGA\ Met-Val-Ala-Ala-Stop 5 AUG CAC AUU UAG\ Met-His-Ile-Stop 6 AUG UCU CCC GUA UAA\ Met-Ser-Pro-Val-Stop 7 AUG AAU AUU CUC CUC CCC ACC UAA\ Met-Asn-Ile-Leu-Leu-Pro-Thr-Stop 8 AUG GUU AAU AAU GCA UAG\ Met-Val-Asn-Asn-Ala-Stop 9 AUG UUC UUU GGG UGA\ Met-Phe-Phe-Gly-Stop 10 AUG CGU CGG AAC ACA UAA\ Met-Arg-Arg-Asn-Thr-Stop =Vanilla =Fudge Swirl =Chocolate Chips =Marshmallows =Twix =Chocolate Syrup =Oreos =Snickers =Shaped Sprinkles =Strawberry Sundae 4 1 AUG GUA UCC CUC UAA\ Met-Val-Ser-Leu-Stop 2 AUG UAC CCC CAA CAG AAA UAA\ Met-Tyr-Pro-Gln-Gln-Lys-Trp-Stop 3 AUG UUA UUG CUG CCC UAG\ Met-Leu-Leu-Leu-Pro-Stop ©copyright 2014-all rights reserved www.cpalms.org =Chocolate =Fudge Swirl =M&M’s 4 AUG GUG GCA GCU UGA\ Met-Val-Ala-Ala-Stop 5 AUG CAU AUU UAA\ Met-His-Ile-Stop 6 AUG UCU CCC GUA UGA\ Met-Ser-Pro-Val-Stop 7 AUG AAC AUU CCU CCC CCA ACA UAA\ Met-Asn-Ile-Pro-Pro-Pro-Thr-Stop 8 AUG GUA AAC AAU GCC UAA\ Met-Val-Asn-Asn-Ala-Stop 9 AUG UUU UCU GGC UGA\ Met-Phe-Ser-Gly-Stop 10 AUG AGA AGG AAU ACG UAA\ Met-Arg-Asn-Thr-Stop ©copyright 2014-all rights reserved www.cpalms.org =Marshmallows =Twix =Chocolate Syrup =Jelly Beans =Snickers Bites =Round Sprinkles =Strawberry