Download How does DNA determine the traits of an Ice Cream Sundae

How does DNA determine the traits of an Ice Cream Sundae?
Purpose is to examine the DNA sequence of an ice cream sundae
Ice Cream Sundaes only have one chromosome with 10 genes on it.
Procedure:
You must analyze the genes of its DNA to determine what traits
the sundae has and then create a model of your ice cream sundae.
These genes must be assembled in order 
1. Transcribe your DNA sequence into a mRNA sequence (do not
forget to break your sequence into codon segments).
2. Use a genetic code chart to translate your mRNA codons into
amino acids.
3. Use the amino acid sequence to determine what trait
(phenotype) each gene will express for your ice cream sundae.
4. After completing step 3 – ask your teacher to verify the
accuracy of your ice cream sundae gene expression.
For simplicity, the gene sequences are much smaller than –real- gene sequences found in living organisms. Each gene
has two versions that result in a different trait being expressed on a sundae.
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Genes
Amino Acid Sequence
Gene 1 – ice cream
type
MET-VAL- SER- LEU-STOP
MET-VAL- SER- LYS-STOP
Gene 2 – ice cream
type
Gene 3 –
MET-TYR – PRO- GLU- GLU- LYSSTOP
MET-VAL- PRO- THR- GLN- LYSSTOP
MET-LEU- LEU- LEU- PRO-STOP
MET-LEU- LEU- SER- ALA-STOP
Gene 4 –
MET-ALA- VAL- VAL-STOP
MET-VAL- ALA- ALA-STOP
Gene 5 –
MET-HIS – ILE-STOP
METH-HIS- HIS-STOP
Gene 6 –
MET-SER- PRO- VAL-STOP
MET-VAL- PHE- TYR-STOP
Gene 7 –
Gene 8 –
MET-ASN- ILE- LEU- LEU- PROTHR-STOP
MET-ASN- ILE- PRO- PRO- PROTHR-STOP
MET-VAL- ASN- ASN- ALA-STOP
MET-ASN- ASN- ASN- ALA-STOP
Gene 9 –
MET-PHE- SER- GLY-STOP
MET-PHE- PHE- GLY-STOP
Gene 10 –
MET-ARG- TYR- CYS- LYS-STOP
MET-ARG- ARG- ASN- THR-STOP
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Description
Chocolate
Vanilla
Fudge Swirl
Neopolitan
M&M’s
Chocolate Chips
Redi-Whip
Marshmallows
Twix
Kit Kat
Chocolate Syrup
Caramel Syrup
Oreos
Jelly Beans
Snickers Bites
Reeses Pieces
Round Sprinkles
Shaped (not round) Sprinkles
Cherry
Strawberry
(Level 4)DNA with 10 gene sequences each with a start and stop
codon including “junk”
Sundae 1
TACCAGTCGTTTATTTAAAGCCATATTTACATGGGGGTTGTCTTTACTTA
AAGCCATATTTACGAGAATTCACGCATCTAAAGCCATATTTACCGACAAC
ACATTTAAAGCCATATTTACGTAGTAATCTAAAGCCATATTTACCAAAA
AATGATTTAAAGCCATATTTACTAATAGAATGACGGGTGGATTTAAAGC
CATATTTACTTATTGTTACGGATCTAAAGCCATATTTACAAAAGACCGAC
TTAAAGCCATATTTACGCGATGACATTTATTTAAAGCCATATT
Sundae 2
TACCATAGATTTATTTAAAGCCATATTTACCAAGGATGAGTTTTCACTT
AAAGCCATATTTACGAAGAGGAGGGGATCTAAAGCCATATTTACCAACG
CCGAATTTAAAGCCATATTTACGTAGTGATCTAAAGCCATATTTACCATA
AAATAACTTAAAGCCATATTTACTTATAAGAAGACGGGTGTACTTAAAG
CCATATTTACTTATTATTACGTATCTAAAGCCATATTTACAAGAGCCCGA
TTTAAAGCCATATTTACGCGGCGTTATGAATTTAAAGCCATATT
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Sundae 3
TACCAGTCGTTTATTTAAAGCCATATTTACATGGGGGTTGTCTTTACTTA
AAGCCATATTTACGAGAATTCAGCGATCTAAAGCCATATTTACCAACGCC
GAACTTAAAGCCATATTTACGAGTAAATCTAAAGCCATATTTACAGAGG
GCATATTTAAAGCCATATTTACTTATAAGAGGAGGGGTGGATTTAAAGC
CATATTTACCAATTATTACGTATCTAAAGCCATATTTACAAGAAACCCAC
TTAAAGCCATATTTACGCAGCCTTGTGTATTTAAAGCCATATT
Sundae 4
TACCATAGGGAGATTTAAAGCCATATTTACATGGGGGTTGTCTTTATTT
AAAGCCATATTTACAATAACGACGGGATCTAAAGCCATATTTACCACCGT
CGAACTTAAAGCCATATTTACGTATAAATTTAAAGCCATATTTACAGAG
GGCATACTTAAAGCCATATTTACTTGTAAGGAGGGGGGTGTATTTAAAG
CCATATTTACCATTTGTTACGGATTTAAAGCCATATTTACAAAAGACCGA
CTTAAAGCCATATT TACTCTTCCTTATGCATT TAAAGCCATATT
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(Level 3)DNA with 10 gene sequences each with a start and stop
codon
Sundae 1
TACCAGTCGTTTATTTACATGGGGGTTGTCTTTACTTACGAGAATTCACG
CATCTACCGACAACACATTTACGTAGTAATCTACCAAAAAATGATTTACT
AATAGAATGACGGGTGGATTTACTTATTGTTACGGATCTACAAAAGACC
GACTTACGCGATGACATTTATT
Sundae 2
TACCATAGATTTATTTACCAAGGATGAGTTTTCACTTACGAAGAGGAGG
GGATCTACCAACGCCGAATTTACGTAGTGATCTACCATAAAATAACTTAC
TTATAAGAAGACGGGTGTACTTACTTATTATTACGTATCTACAAGAGCC
CGATTTACGCGGCGTTATGAATT
Sundae 3
TACCAGTCGTTTATTTACATGGGGGTTGTCTTTACTTACGAGAATTCAGC
GATCTACCAACGCCGAACTTACGAGTAAATCTACAGAGGGCATATTTACT
TATAAGAGGAGGGGTGGATTTACCAATTATTACGTATCTACAAGAAACC
CACTTACGCAGCCTTGTGTATT
Sundae 4
TACCATAGGGAGATTTACATGGGGGTTGTCTTTATTTACAATAACGACG
GGATCTACCACCGTCGAACTTACGTATAAATTTACAGAGGGCATACTTAC
TTGTAAGGAGGGGGGTGTATTTACCATTTGTTACGGATTTACAAAAGAC
CGACTTACTCTTCCTTATGCATT
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(Level 2)DNA with 10 gene sequences each with a start and stop
codon
Sundae 1
1
2
3
4
5
6
7
8
9
10
TACCAGTCGTTTATT
TACATGGGGGTTGTCTTTACT
TACGAGAATTCACGCATC
TACCGACAACACATT
TACGTAGTAATC
TACCAAAAAATGATT
TACTAATAGAATGACGGGTGGATT
TACTTATTGTTACGGATC
TACAAAAGACCGACT
TACGCGATGACATTTATT
Sundae 2
1
2
3
4
5
6
7
8
9
10
TACCATAGATTTATT
TACCAAGGATGAGTTTTCACT
TACGAAGAGGAGGGGATC
TACCAACGCCGAATT
TACGTAGTGATC
TACCATAAAATAACT
TACTTATAAGAAGACGGGTGTACT
TACTTATTATTACGTATC
TACAAGAGCCCGATT
TACGCGGCGTTATGAATT
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Sundae 3
1
2
3
4
5
6
7
8
9
10
TACCAGTCGTTTATT
TACATGGGGGTTGTCTTTACT
TACGAGAATTCAGCGATC
TACCAACGCCGAACT
TACGAGTAAATC
TACAGAGGGCATATT
TACTTATAAGAGGAGGGGTGGATT
TACCAATTATTACGTATC
TACAAGAAACCCACT
TACGCAGCCTTGTGTATT
Sundae 4
1
2
3
4
5
6
7
8
9
10
TACCATAGGGAGATT
TACATGGGGGTTGTCTTTATT
TACAATAACGACGGGATC
TACCACCGTCGAACT
TACGTATAAATT
TACAGAGGGCATACT
TACTTGTAAGGAGGGGGGTGTATT
TACCATTTGTTACGGATT
TACAAAAGACCGACT
TACTCTTCCTTATGCATT
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(Level 1) Gene sequences in mRNA code – codons separated
Sundae 1
AUG GUC AGC AAA UAA\ AUG UAC CCC CAA CAG AAA UGA\ MET CUC
UUA AGU GCG UAG\ MET GCU GUU GUG UAA\ MET CAU CAU UAG\
MET GUU UUU UAC UAA\ MET AUU AUC UUA CUG CCC ACC UAA\
MET AAU AAC AAU GCC UAG\ MET UUU UCU GGC UGA\ MET CGC
UAC UGU AAA UAA
Sundae 2
AUG GUA UCU AAA UAA\ AUG GUU CCU ACU CAA AAG UGA\ AUG CUU
CUC CUC CCC UAG\ AUG GUU GCG GCU UAA\ AUG CAU CAC UAG\ AUG
GUA UUU UAU UGA\ AUG AAU AUU CUU CUG CCC ACA UGA\ AUG AAU
AAU AAU GCA UAG\ AUG UUC UCG GGC UAA\ AUG CGC CGC AAU ACU
UAA
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Sundae 3
AUG GUC AGC AAA UAA\ AUG UAC CCC CAA CAG AAA UGA\ AUG CUC
UUA AGU CGC UAG\ AUG GUU GCG GCU UGA\ AUG CAC AUU UAG\
AUG UCU CCC GUA UAA\ AUG AAU AUU CUC CUC CCC ACC UAA\ AUG
GUU AAU AAU GCA UAG\ AUG UUC UUU GGG UGA\ AUG CGU CGG AAC
ACA UAA
Sundae 4
AUG GUA UCC CUC UAA\ AUG UAC CCC CAA CAG AAA UAA\ AUG UUA
UUG CUG CCC UAG\ AUG GUG GCA GCU UGA\ AUG CAU AUU UAA\
AUG UCU CCC GUA UGA\ AUG AAC AUU CCU CCC CCC ACA UAA\ AUG
GUA AAC AAU GCC UAA\ AUG UUU UCU GGC UGA\ AUG AGA AGG AAU
ACG UAA
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Teacher Key
Sundae 1
1 AUG GUC AGC AAA UAA\Met-Val-Thr-Lys-Stop
2 AUG UAC CCC GAA GAG AAA UGA\Met-Tyr-Pro-Glu-Glu-Lys-Stop
3 AUG CUC UUA AGU GCG UAG\ Met-Leu-Leu-Ser-Ala-Stop
4 AUG GCU GUU GUG UAA\ Met-Ala-Val-Val-Stop
5 AUG CAU CAU UAG\ Met-His-His-Stop
6 AUG GUU UUU UAC UAA\ Met-Val-Phe-Tyr-Stop
7 AUG GAU AUC UUA CUG CCC ACC UAA\ Met-Asp-Ile-Leu-Leu-Pro-Thr-Stop
8 AUG AAU AAC AAU GCC UAG\ Met-Asn-Asn-Asn-Ala-Stop
9 AUG UUU UCU GGC UGA\ Met-Phe-Ser-Gly-Stop
10 AUG CGC UAC UGU AAA UAA\ Met-Arg-Tyr-Cys-Lys-Stop
=Vanilla
=Fudge Swirl
=Chocolate Chips
=Redi-Whip
=Kit Kat
=Caramel Syrup
=Oreo
=Reeses Pieces
=Round Sprinkles
=Cherry
Sundae 2
1 AUG GUA UCU AAA UAA\ Met-Val-Ser-Lys-Stop
2 AUG GUU CCU ACU CAA AAG UGA\ Met-Val-Pro-Thr-Gln-Lys-Stop
3 AUG CUU CUC CUC CCC UAG\ Met-Leu-Leu-Leu-Pro-Stop
4 AUG GUU GCG GCU UAA\ Met-Val-Ala-Ala-Stop
5 AUG CAU CAC UAG\ Met-His-His-Stop
6 AUG GUA UUU UAU UAG\ Met-Val-Phe-Tyr-Stop
7 AUG AAU AUU CUU CUG CCC ACA UGA\ Met-Asn-Ile-Leu-Leu-Pro-Thr-Stop
8 AUG AAU AAU AAU GCA UAG\ Met-Asn-Asn-Asn-Ala-Stop
9 AUG UUC UCG GGC UAA\ Met-Phe-Ser-Gly-Stop
10 AUG CGC CGC AAU ACU UAA\Met-Arg-Arg-Asn-Thr-Stop
=Vanilla
=Neopolitan
=M&M’s
=Marshmallows
=Kit Kat
=Caramel Syrup
=Oreos
=Reeses Pieces
=Round Sprinkles
=Strawberry
Sundae 3
1 AUG GUC AGC AAA UAA\ Met-Val-Ser-Lys-Stop
2 AUG UAC CCC GAA GAG AAA UGA\ Met-Tyr-Pro-Glu-Glu-Lys-Stop
3 AUG CUC UUA AGU CGC UAG\ Met-Leu-Leu-Ser-Ala-Stop
4 AUG GUU GCG GCU UGA\ Met-Val-Ala-Ala-Stop
5 AUG CAC AUU UAG\ Met-His-Ile-Stop
6 AUG UCU CCC GUA UAA\ Met-Ser-Pro-Val-Stop
7 AUG AAU AUU CUC CUC CCC ACC UAA\ Met-Asn-Ile-Leu-Leu-Pro-Thr-Stop
8 AUG GUU AAU AAU GCA UAG\ Met-Val-Asn-Asn-Ala-Stop
9 AUG UUC UUU GGG UGA\ Met-Phe-Phe-Gly-Stop
10 AUG CGU CGG AAC ACA UAA\ Met-Arg-Arg-Asn-Thr-Stop
=Vanilla
=Fudge Swirl
=Chocolate Chips
=Marshmallows
=Twix
=Chocolate Syrup
=Oreos
=Snickers
=Shaped Sprinkles
=Strawberry
Sundae 4
1 AUG GUA UCC CUC UAA\ Met-Val-Ser-Leu-Stop
2 AUG UAC CCC CAA CAG AAA UAA\ Met-Tyr-Pro-Gln-Gln-Lys-Trp-Stop
3 AUG UUA UUG CUG CCC UAG\ Met-Leu-Leu-Leu-Pro-Stop
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=Chocolate
=Fudge Swirl
=M&M’s
4 AUG GUG GCA GCU UGA\ Met-Val-Ala-Ala-Stop
5 AUG CAU AUU UAA\ Met-His-Ile-Stop
6 AUG UCU CCC GUA UGA\ Met-Ser-Pro-Val-Stop
7 AUG AAC AUU CCU CCC CCA ACA UAA\ Met-Asn-Ile-Pro-Pro-Pro-Thr-Stop
8 AUG GUA AAC AAU GCC UAA\ Met-Val-Asn-Asn-Ala-Stop
9 AUG UUU UCU GGC UGA\ Met-Phe-Ser-Gly-Stop
10 AUG AGA AGG AAU ACG UAA\ Met-Arg-Asn-Thr-Stop
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=Marshmallows
=Twix
=Chocolate Syrup
=Jelly Beans
=Snickers Bites
=Round Sprinkles
=Strawberry